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Image Search Results
Journal: Breast Cancer Research : BCR
Article Title: Tumor necroptosis-mediated shedding of cell surface proteins promotes metastasis of breast cancer by suppressing anti-tumor immunity
doi: 10.1186/s13058-023-01604-9
Figure Lengend Snippet: Necroptosis promotes tumor metastasis by activating cell surface proteases. a , b ELISA of soluble JAM-A, CD138 and E-cad in TIF( a ) and serum ( b ) from WT and Mlkl KO MMTV-PyMT mice at 15 weeks ( n = 6, each). c , d ELISA of soluble JAM-A, CD138 and E-cad in TIF ( c ) and serum ( d ) from MMTV-PyMT mice treated with vehicle or GW280264X ( n = 5, each). e qPCR analysis of IFN-γ mRNA expression in tumor-infiltrating CD8+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X ( n = 5, each; left panel). Flow cytometry analysis of tumor-infiltrating GZMB+ CD8+ T cells in MMTV-PyMT mice treated with vehicle or GW280264X ( n = 5, each; right panel). f qPCR analysis of IFN-γ and T-bet mRNA expression in tumor-infiltrating CD4+ T cells of MMTV-PyMT mice treated with vehicle or GW280264X ( n = 5, each). g Flow cytometry analysis of peripheral blood GZMB+ CD8+ T cells and IFN-γ+ CD4+ T cells MMTV-PyMT mice treated with vehicle or GW280264X ( n = 5, each). h Left panel shows the representative images of H&E-stained lung sections from MMTV-PyMT mice treated with vehicle or GW280264X.Scale bar, 2 mm. Right panel shows the quantification of metastatic foci in lungs from MMTV-PyMT mice treated with vehicle or GW280264X ( n = 5, each). Data presented as the mean ± sem and p value was determined by two-tailed t test ( a – h )
Article Snippet: Samples were then analyzed for mouse JAM-A (ab277080, Abcam),
Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Staining, Two Tailed Test