Journal:

Article Title: Effects of Inoculum and ?-Lactamase Activity in AmpC- and Extended-Spectrum ?-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Clinical Isolates Tested by Using NCCLS ESBL Methodology

doi: 10.1128/JCM.42.1.269-275.2004

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: For strains that contained multiple β-lactamases or clinical strains with low β-lactamase expression, the ESBL was purified from the following E. coli cloning strains in which the ESBL gene is on a plasmid: OC4249 for ACT-1, OC5032 for SHV-18, and OC4107 for TEM-26. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain designation β-Lactamase(s) Functional group(s) a β-Lactamase sp act b Reference Original strain designation E. coli ATCC 25922 None None 16 ATCC 35218 TEM-1 2b 700 OC4075 TEM-1 2b 7,500 10 TEM OC4229 TEM-1, SHV-12 2b, 2be 3,800 32 EC 2859 case 12 OC6028 TEM-1, CTX-M-10 2b, 2be 2,500 18 97/38582 OC4087 TEM-3 2be 900 27 SC15011 CF 102 OC6042 TEM-10 2be 370 33 166 OC4227 TEM-12 2be 1,700 32 EC 1924 case 10 OC6043 TEM-28 2be 2,500 3 2300 OC6044 TEM-43 2be 4,100 33 156 OC4138 AmpC 1 700 32 EC 3102 case 3 OC4136 AmpC 1 1,300 32 EC 1201 case 1 OC4249 ACT-1 c 1 12,000 This work Transformant K. pneumoniae ATCC 13883 None None 0.35 ATCC 700603 SHV-18 2be 240 25 K6 OC4244 TEM-1, SHV-5 2b, 2be 690 32 KP 3160 case 20 OC4239 TEM-6, SHV-1 2be, 2b 750 32 KP 2679 case 17 OC4110 TEM-10, SHV-1 2be, 2b 850 26 2351 OC4105 TEM-26, SHV-1 2be, 2b 970 14 SC 15923 OC4074 TEM-1, MIR-1 c 2b, 1 12,000 20 96D OC4250 ACT-1, 2 TEM type, 2 SHV type 1, 2b, 2be, unknown 17,000 4 MCQ-95 OC5064 FOX-5 c , TEM type, SHV-11 1, 2b 3,200 24 OC5064 K. oxytoca OC4076 K-1 2be 14,000 23 SC10436 Open in a separate window a Functional group according to reference 8. b Specific activity reported in nanomoles of nitrocefin hydrolyzed per minute per milligram of protein. c Plasmid-encoded AmpC β-lactamase.

Techniques: Functional Assay

Journal:

Article Title: Effects of Inoculum and ?-Lactamase Activity in AmpC- and Extended-Spectrum ?-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Clinical Isolates Tested by Using NCCLS ESBL Methodology

doi: 10.1128/JCM.42.1.269-275.2004

Figure Lengend Snippet: E. coli MIC values for extended-spectrum cephalosporins and aztreonam

Article Snippet: For strains that contained multiple β-lactamases or clinical strains with low β-lactamase expression, the ESBL was purified from the following E. coli cloning strains in which the ESBL gene is on a plasmid: OC4249 for ACT-1, OC5032 for SHV-18, and OC4107 for TEM-26. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain designation β-Lactamase(s) Functional group(s) a β-Lactamase sp act b Reference Original strain designation E. coli ATCC 25922 None None 16 ATCC 35218 TEM-1 2b 700 OC4075 TEM-1 2b 7,500 10 TEM OC4229 TEM-1, SHV-12 2b, 2be 3,800 32 EC 2859 case 12 OC6028 TEM-1, CTX-M-10 2b, 2be 2,500 18 97/38582 OC4087 TEM-3 2be 900 27 SC15011 CF 102 OC6042 TEM-10 2be 370 33 166 OC4227 TEM-12 2be 1,700 32 EC 1924 case 10 OC6043 TEM-28 2be 2,500 3 2300 OC6044 TEM-43 2be 4,100 33 156 OC4138 AmpC 1 700 32 EC 3102 case 3 OC4136 AmpC 1 1,300 32 EC 1201 case 1 OC4249 ACT-1 c 1 12,000 This work Transformant K. pneumoniae ATCC 13883 None None 0.35 ATCC 700603 SHV-18 2be 240 25 K6 OC4244 TEM-1, SHV-5 2b, 2be 690 32 KP 3160 case 20 OC4239 TEM-6, SHV-1 2be, 2b 750 32 KP 2679 case 17 OC4110 TEM-10, SHV-1 2be, 2b 850 26 2351 OC4105 TEM-26, SHV-1 2be, 2b 970 14 SC 15923 OC4074 TEM-1, MIR-1 c 2b, 1 12,000 20 96D OC4250 ACT-1, 2 TEM type, 2 SHV type 1, 2b, 2be, unknown 17,000 4 MCQ-95 OC5064 FOX-5 c , TEM type, SHV-11 1, 2b 3,200 24 OC5064 K. oxytoca OC4076 K-1 2be 14,000 23 SC10436 Open in a separate window a Functional group according to reference 8. b Specific activity reported in nanomoles of nitrocefin hydrolyzed per minute per milligram of protein. c Plasmid-encoded AmpC β-lactamase.

Techniques:

Journal: Ceramics

Article Title: Osteogenic Enhancement of Zirconia-Toughened Alumina with Silicon Nitride and Bioglass®

doi: 10.3390/ceramics2040043

Figure Lengend Snippet: Figure 8. (a) SEM picture and EDS (Energy Dispersive X-ray Spectroscopy) maps of (b) Si, (c) Al, and (d) Ca collected in the same region shown in Figure 7 after 1-week in vitro exposure to SaOS-2 osteoblasts in biological environment.

Article Snippet: Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDS) (JSM-700 1F, JEOL, Tokyo, Japan) were used to acquire high-resolution images and chemical composition maps of Si3N4-coated substrates.

Techniques: Spectroscopy, In Vitro

Journal: Micromachines

Article Title: Suppression of Surface Waviness Error of Fresnel Micro-Structured Mold by Using Non-Integer Rotation Speed Ratio in Parallel Grinding Process

doi: 10.3390/mi11070652

Figure Lengend Snippet: Grinding experiment conditions.

Article Snippet: Digital microscope , Dino-Lite ® , AM7515MT8A (700×–900×), AM7115MZTL (10×–140×).

Techniques: Microscopy

Journal: Physical Review Letters

Article Title: Ostwald Ripening in Nanoalloys: When Thermodynamics Drives a Size-Dependent Particle Composition

doi: 10.1103/physrevlett.105.255901

Figure Lengend Snippet: FIG. 1. TEM images of CoPt NPs with the corresponding particle size distribution in the insets. (a) As-grown NPs (sample A), (b) NPs after 1 h at 650 C (sample B), and (c) NPs after 1 h at 750 C (sample C).

Article Snippet: Reference samples with the same nominal thickness of pure Pt and pure Co NPs were also grown and annealed for 1 h at 700 C. TEM experiments were carried out on a JEM-2100F field-emission electron microscope operating at 200 kV and equipped with a JED 2300T EDX analyzer from JEOL.

Techniques:

Journal: Journal of experimental & clinical cancer research : CR

Article Title: Gasdermin B over-expression modulates HER2-targeted therapy resistance by inducing protective autophagy through Rab7 activation.

doi: 10.1186/s13046-022-02497-w

Figure Lengend Snippet: Fig. 2 GSDMB-high cells show an increased autophagic flux in response to lapatinib. A-B GSDMB and LC3B protein levels in shNTC, shGB1 and shGB2 HCC1954 (A) and OE19 (B) cells treated with lapatinib (Lap, 2 µM and 0.7 µM, respectively) and/or CQ (10 µM and 50 µM, respectively) for 72 h. Quantification of the relative LC3B-II expression was conducted as described before [34, 35]. C Representative transmission electron microscopy images of shNTC and shGB2 HCC1954 cells treated with the treatment regimens indicated in (A). Quantification of the relative volume density of autophagic vacuoles is shown on the right. At least 25 cells were analyzed per experimental condition. D-E Western blot analysis of GSDMB and LC3B (left panels) in HCC1954 LR (D) and OE19 LR (E) cells and their respective controls (C) treated with or without CQ (10 µM and 50 µM, respectively) for 72 h. LC3B expression (green) analysis by confocal microscopy (right panels) in HCC1954 LR (D) and OE19 LR (E) cells and their controls (C) treated with or without CQ at the concentrations indicated in (A-B). Representative confocal microscopy images were shown, scale bar, 10 µm. Nuclei were counterstained with DAPI. F GSDMB and LC3B protein levels in GSDMB-siRNA-silenced HCC1954 LR cells treated with or without 10 µM CQ for 72 h. Quantification of LC3B-II expression (showed on the right of panels, A-B, D-F) was carried out by densitometric scanning and normalized to GAPDH expression following previous methods [34, 35]. Statistical significance was determined by two-tailed unpaired t-test (*P < 0.05; **P < 0.01). Data are shown as the mean ± s.e.m. Three independent experiments with similar results were performed. NTC, non-targeting control. LR, Lapatinib resistant cells. CQ, chloroquine. Lap, lapatinib

Article Snippet: Cell culture and in vitro assays HCC1954 (derived from a human invasive ductal breast carcinoma), NCI-N87 (human gastric adenocarcinoma) and HEK293T cell lines were obtained from the American Type Cell Culture (ATCC) and OE19 (human esophageal adenocarcinoma) cell line from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ).

Techniques: Expressing, Transmission Assay, Electron Microscopy, Western Blot, Confocal Microscopy, Two Tailed Test, Control