Journal: Sensors and Actuators B: Chemical

Article Title: Phenotyping antibiotic resistance with single-cell resolution for the detection of heteroresistance

doi: 10.1016/j.snb.2018.05.047

Figure Lengend Snippet: Fig. 1. Scheme and optical images of the microfluidic process flow. The process consists of three main parts: (a) droplet generation and encapsulation of bac- teria with antibiotic, (b) pico-injection of alamarBlue into droplets, and (c) the detection of fluorescence from the drops.

Article Snippet: [28] We used ampicillin (Alfa Aesar) as the model antibiotic for Figs. 2–4, norfloxacin (Chem-Impex Int’l Inc.), kanamycin (bioWORLD), and tetracycline (Sigma-Aldrich) as model antibiotics for Fig. 5, and ampicillin, norfloxacin, and kanamycin as model antibiotics for Fig. 6.

Techniques: Encapsulation, Injection

Journal:

Article Title: Two Similar Gene Clusters Coding for Enzymes of a New Type of Aerobic 2-Aminobenzoate (Anthranilate) Metabolism in the Bacterium Azoarcus evansii

doi: 10.1128/JB.183.18.5268-5278.2001

Figure Lengend Snippet: Primary structure of ACMR (ORF 1 product; AbmA), consisting of two domains and a short spacer region, and percent identities (Ident.) and similarities (Pos.) with enzymes in the databases. Databases and accession numbers are as follows: Streptomyces coelicolor putative hydroxylase, EMBL CAB42932.1; Chromobacterium violaceum hydroxylase VioD, DBJ BAA84785.1; Bordetella pertussis unknown protein, GB ACC46266; C. violaceum hydroxylase VioC, DBJ BAA84784.1; Bacillus subtilis salicylate hydroxylase, GB AAA64522; Deinococcus radiodurans NADH-dependent flavin oxidoreductase, GB AAF11740.1; Pseudomonas putida xenobiotic reductase, GB AFF02538.1; B. subtilis putative NADH-dependent flavin oxidoreductase, SP P54550; Thermoanaerobacter brockii NADH oxidase, PIR S35706; E. coli N-ethylmaleimide reductase, SP P77258; Saccharomyces cerevisiae old yellow enzyme, DBJ BAA20121.

Article Snippet: Databases and accession numbers are as follows: Streptomyces coelicolor putative hydroxylase, EMBL CAB42932 .1; Chromobacterium violaceum hydroxylase VioD, DBJ BAA84785 .1; Bordetella pertussis unknown protein, GB ACC46266 ; C . violaceum hydroxylase VioC, DBJ BAA84784 .1; Bacillus subtilis salicylate hydroxylase, GB AAA64522 ; Deinococcus radiodurans NADH-dependent flavin oxidoreductase, GB AAF11740 .1; Pseudomonas putida xenobiotic reductase, GB AFF02538 .1; B. subtilis putative NADH-dependent flavin oxidoreductase, SP P54550 ; Thermoanaerobacter brockii NADH oxidase, PIR S35706 ; E. coli N -ethylmaleimide reductase, SP P77258 ; Saccharomyces cerevisiae old yellow enzyme, DBJ BAA20121 . table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 ORF (protein) Similar protein % Identity % Similarity ACR no. 2 (AbmB) a Domain of daunorubicin-doxorubicin polyketide synthase ( Streptomyces peucetius ) 37 49 L35560 , GB AAA65204 d -β-hydroxybutyrate dehydrogenase ( Rhodobacter sp. DSMZ 12077) 36 47 AF037323 , GB AAD42688 Ketoacyl reductase ( Streptomyces galilaeus ) 37 47 D14040 , DBJ BAA03128 3-Hydroxybutyrate dehydrogenase ( Pseudomonas aeruginosa ) 36 50 AE004626 , GB AAG05391 3 (AbmC) Putative acyl-CoA isomerase [ Streptomyces coelicolor A3(2)] 33 45 AL390975 , EMBL CAC01345 PhaB ( Pseudomonas putida ) 29 45 AF029714 , GB AAC24330 Enoyl-CoA isomerase ( E. coli ) 27 45 X97452 , EMBL CAA66096 2-Cyclohexenylcarbonyl-CoA isomerase ( Streptomyces collinus ) 31 44 AF268489 , GB AAF73478 4 (AbmD) Butyryl-CoA dehydrogenase ( Thermoanaerobacterium thermosaccharolyticum ) 41 52 Z92974 , EMBL CAB07496 Probable acyl-CoA dehydrogenase ( P. aeruginosa ) 36 51 AE004683 , GB AAG05940 Acyl-CoA dehydrogenase ( Mycobacterium leprae ) 34 53 U00012 , GB AAA85936 Acyl-CoA dehydrogenase ( Bacillus halodurans ) 31 49 AP001520 , DBJ BAB07518 5 (AbmE) Translational inhibitor protein p14.5 ( Homo sapiens ) 30 51 X95384 , EMBL CAA64670 Hypothetical protein ( Synechocystis sp.)

Techniques:

Journal: Pharmaceutics

Article Title: Terahertz Spectroscopy for Non-Destructive Solid-State Investigation of Norfloxacin in Paper Tablets after Wet Granulation

doi: 10.3390/pharmaceutics15071786

Figure Lengend Snippet: Normalized X-ray powder diffractogram ( left panel) and THz absorption spectra ( right panel) of ( A ) crystalline norfloxacin, ( B ) crystalline sucrose, ( C ) blank smartFilm tablet, ( D ) physical mixture tablet containing 20 w / w % sucrose and 10 w / w % norfloxacin, ( E ) granulated norfloxacin-loaded smartFilm tablet and ( F ) non-granulated norfloxacin-loaded smartFilm tablet. The horizontal lines mark the most prominent diffraction peaks (panels on the left, XRD) or the characteristic absorption features (panels on the right, THz TDS) of crystalline norfloxacin (dashed blue lines) and sucrose (dotted red line). For clarity, only one diffractogram/spectrum is shown per sample type.

Article Snippet: Norfloxacin (95%, CAS number: 70458-96-7) was acquired from abcr GmbH (Karlsruhe, Germany) and used as received.

Techniques:

Journal: Pharmaceutics

Article Title: Terahertz Spectroscopy for Non-Destructive Solid-State Investigation of Norfloxacin in Paper Tablets after Wet Granulation

doi: 10.3390/pharmaceutics15071786

Figure Lengend Snippet: Fitted absorption coefficient (Abs. coeff.) of ( A ) physical mixture tablet containing 10 w / w % crystalline norfloxacin, ( B ) granulated norfloxacin-loaded smartFilm tablet, ( C ) non-granulated norfloxacin-loaded smartFilm tablet and ( D ) crystalline norfloxacin. The solid lines represent the original spectra, the black dotted lines the fitted background absorption and the dashed lines the fitted spectra. In ( A – C ) the spectral region around the most prominent absorption peak in the investigated range is shown and the shaded areas relate to the amount of crystalline norfloxacin in the samples. In ( D ) the shaded areas illustrate the fitted peaks. The grey box indicates the frequency region that was not considered for the analysis of the physical mixtures and smartFilm tablets. ( E ) Estimated crystalline (Est. cryst.) norfloxacin content in physical mixtures, granulated and non-granulated smartFilm tablets. The linear fit regarding the physical mixtures is shown as the grey dashed-dotted line. Inset: estimated crystalline norfloxacin content in granulated (red square) and non-granulated (blue circle) smartFilm tablets. The error bars represent the standard deviation of the assessed crystalline norfloxacin content of the three samples per sample type. Note that a closer look at the determined crystalline content of the granulated and non-granulated tablets shown in the inset in ( E ) reveals that the values clearly differ.

Article Snippet: Norfloxacin (95%, CAS number: 70458-96-7) was acquired from abcr GmbH (Karlsruhe, Germany) and used as received.

Techniques: Standard Deviation

Journal: Pharmaceutics

Article Title: Terahertz Spectroscopy for Non-Destructive Solid-State Investigation of Norfloxacin in Paper Tablets after Wet Granulation

doi: 10.3390/pharmaceutics15071786

Figure Lengend Snippet: Extracted Gaussian peak properties for the individual absorption peaks of crystalline norfloxacin including their amplitude ( G ), frequency position ( v 0 ) and width (Δ v ).

Article Snippet: Norfloxacin (95%, CAS number: 70458-96-7) was acquired from abcr GmbH (Karlsruhe, Germany) and used as received.

Techniques:

Journal: Pharmaceutics

Article Title: Terahertz Spectroscopy for Non-Destructive Solid-State Investigation of Norfloxacin in Paper Tablets after Wet Granulation

doi: 10.3390/pharmaceutics15071786

Figure Lengend Snippet: ( A ) Normalized (Norm.) X-ray powder diffractogram and ( B ) THz absorption coefficient (Abs. coeff.) of a physical mixture containing 2.5 w / w % norfloxacin. The dashed blue horizontal lines mark the position of the most prominent diffraction features ( A , XRD) or the characteristic absorption peaks ( B , THz TDS) of crystalline norfloxacin (see panels ( A ) in ).

Article Snippet: Norfloxacin (95%, CAS number: 70458-96-7) was acquired from abcr GmbH (Karlsruhe, Germany) and used as received.

Techniques: