50582-M08H Search Results


ephb4  (Sino Biological)
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Sino Biological ephb4
Purported functional effects of administered recombinant proteins ephrin-B1-Fc, EphB1-Fc, and <t> EphB4. </t>
Ephb4, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/50582-M08H/pmc06606944-128-0-3?v=Sino+Biological
Average 94 stars, based on 1 article reviews
ephb4 - by Bioz Stars, 2026-08
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Purported functional effects of administered recombinant proteins ephrin-B1-Fc, EphB1-Fc, and EphB4.

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: Purported functional effects of administered recombinant proteins ephrin-B1-Fc, EphB1-Fc, and EphB4.

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques: Functional Assay, Recombinant

Experimental design with characteristics of each experimental group.

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: Experimental design with characteristics of each experimental group.

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques:

Effects of EphB/ephrinB ligands on 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced inflammatory responses. Disease Activity Index (A) , macroscopic score (B) , colonic length (C) , colonic thickness (D) , colonic myeloperoxidase (MPO) (E) , and lung MPO (F) activity assessed in vehicle-treated normal mice (N) and in TNBS-treated mice administered with vehicle (CNT), EphB1-Fc 30 µg/kg (EphB1), ephrin-B1-Fc 17 µg/kg (ephrB), EphB4 20 µg/kg ( n = 5–12 independent values per group). * P < 0.05 vs. N mice; # P < 0.05 vs. CNT mice; one-way ANOVA followed by Bonferroni’s post-test.

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: Effects of EphB/ephrinB ligands on 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced inflammatory responses. Disease Activity Index (A) , macroscopic score (B) , colonic length (C) , colonic thickness (D) , colonic myeloperoxidase (MPO) (E) , and lung MPO (F) activity assessed in vehicle-treated normal mice (N) and in TNBS-treated mice administered with vehicle (CNT), EphB1-Fc 30 µg/kg (EphB1), ephrin-B1-Fc 17 µg/kg (ephrB), EphB4 20 µg/kg ( n = 5–12 independent values per group). * P < 0.05 vs. N mice; # P < 0.05 vs. CNT mice; one-way ANOVA followed by Bonferroni’s post-test.

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques: Activity Assay

Histology. Representative hematoxylin–eosin-stained sections of colonic specimens harvested from vehicle-treated normal mice (A) and from TNBS-treated mice administered with vehicle (B) or EphB4 20 µg/kg (C) . TNBS colonic instillation caused mucosal necrosis, neutrophils infiltration, and submucosal edema (indicated by arrows) in vehicle-treated animals not attenuated in EphB4-treated mice (C) . Panel D represents histological damage scoring of colonic sections obtained from vehicle-treated normal mice (▪), CNT mice (•), or EphB4-treated colitic mice (◆) (horizontal bar at the mean value).

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: Histology. Representative hematoxylin–eosin-stained sections of colonic specimens harvested from vehicle-treated normal mice (A) and from TNBS-treated mice administered with vehicle (B) or EphB4 20 µg/kg (C) . TNBS colonic instillation caused mucosal necrosis, neutrophils infiltration, and submucosal edema (indicated by arrows) in vehicle-treated animals not attenuated in EphB4-treated mice (C) . Panel D represents histological damage scoring of colonic sections obtained from vehicle-treated normal mice (▪), CNT mice (•), or EphB4-treated colitic mice (◆) (horizontal bar at the mean value).

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques: Staining, Mouse Assay

Effects of EphB/ephrinB ligands on dextran sulphate sodium (DSS)-induced inflammatory responses. Disease Activity Index (A) , colonic length (B) , colonic thickness (C) , colonic MPO (D) and lung MPO (E) activity assessed in vehicle-treated normal mice (N) and in DSS-treated mice administered with vehicle (CNT), EphB1-Fc 30 µg/kg (EphB1), ephrin-B1-Fc 17 µg/kg (ephrB), EphB4 20 µg/kg ( n = 5–12 independent values per group). * P < 0.05 vs. N mice; # P < 0.05 vs. CNT mice; one-way ANOVA followed by Bonferroni’s post-test.

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: Effects of EphB/ephrinB ligands on dextran sulphate sodium (DSS)-induced inflammatory responses. Disease Activity Index (A) , colonic length (B) , colonic thickness (C) , colonic MPO (D) and lung MPO (E) activity assessed in vehicle-treated normal mice (N) and in DSS-treated mice administered with vehicle (CNT), EphB1-Fc 30 µg/kg (EphB1), ephrin-B1-Fc 17 µg/kg (ephrB), EphB4 20 µg/kg ( n = 5–12 independent values per group). * P < 0.05 vs. N mice; # P < 0.05 vs. CNT mice; one-way ANOVA followed by Bonferroni’s post-test.

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques: Activity Assay

Colonic levels of EphB4 and ephrin-B2 mRNA. (A) Representative agarose gels showing mRNA levels of EphB4, ephrin-B2 and GAPDH from the colon of vehicle-treated normal mice (N; n = 7) and of TNBS-treated mice administered with vehicle (CNT; n = 4) or EphB4 20 µg/kg (EphB; n = 5). Histograms represent the quantification of EphB4 (B) and ephrin-B2 (C) mRNA levels normalized to GAPDH amplification products. Transcript X1 (black bars) and transcript X2 (white bars), encoded by ephrin-B2 gene, were detected and represented (C) . Data are shown as mean ± SEM. * P < 0.05 vs. N mice, one-way ANOVA followed by Bonferroni’s post-test.

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: Colonic levels of EphB4 and ephrin-B2 mRNA. (A) Representative agarose gels showing mRNA levels of EphB4, ephrin-B2 and GAPDH from the colon of vehicle-treated normal mice (N; n = 7) and of TNBS-treated mice administered with vehicle (CNT; n = 4) or EphB4 20 µg/kg (EphB; n = 5). Histograms represent the quantification of EphB4 (B) and ephrin-B2 (C) mRNA levels normalized to GAPDH amplification products. Transcript X1 (black bars) and transcript X2 (white bars), encoded by ephrin-B2 gene, were detected and represented (C) . Data are shown as mean ± SEM. * P < 0.05 vs. N mice, one-way ANOVA followed by Bonferroni’s post-test.

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques: Amplification

Colonic levels of EphB4 and ephrin-B2 proteins. (A) Representative western blots showing the expression of EphB4, ephrin-B2 and β-actin proteins in lysates, obtained from the colon of vehicle-treated normal mice (N) and in TNBS-treated mice administered with vehicle (CNT) or EphB4 20 µg/kg (EphB4). Histograms represent the densitometric analysis of EphB4 (B) and ephrin-B2 (C) blots, normalized to β-actin ( n = 3–4 per group).

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: Colonic levels of EphB4 and ephrin-B2 proteins. (A) Representative western blots showing the expression of EphB4, ephrin-B2 and β-actin proteins in lysates, obtained from the colon of vehicle-treated normal mice (N) and in TNBS-treated mice administered with vehicle (CNT) or EphB4 20 µg/kg (EphB4). Histograms represent the densitometric analysis of EphB4 (B) and ephrin-B2 (C) blots, normalized to β-actin ( n = 3–4 per group).

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques: Western Blot, Expressing

EphB4 attenuated TNBS-induced changes in spleen T-cell profile. Number of T lymphocytes (CD3 + ) (A, B) , CD4 + (C, D) , and CD8 + (E, F) T lymphocytes in the spleen (A, C, E) and in MLN (B, D, F) excised from vehicle-treated normal mice (N) and TNBS-treated mice administered with vehicle (CNT) or EphB4 20 µg/kg (EphB4) ( n = 5–9 independent values per group). * P < 0.05 vs. N mice; # P < 0.05 vs. CNT mice, one-way ANOVA followed by Bonferroni’s post-test.

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: EphB4 attenuated TNBS-induced changes in spleen T-cell profile. Number of T lymphocytes (CD3 + ) (A, B) , CD4 + (C, D) , and CD8 + (E, F) T lymphocytes in the spleen (A, C, E) and in MLN (B, D, F) excised from vehicle-treated normal mice (N) and TNBS-treated mice administered with vehicle (CNT) or EphB4 20 µg/kg (EphB4) ( n = 5–9 independent values per group). * P < 0.05 vs. N mice; # P < 0.05 vs. CNT mice, one-way ANOVA followed by Bonferroni’s post-test.

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques:

Effects of EphB4 on splenic mononuclear cells activation. TNFα production by cultured splenic mononuclear cells incubated with vehicle (Veh), cyclosporine A 1 µg/ml (CsA) or EphB4 (10–100 ng/ml) (EphB4) in the presence (black bars) or absence (white bars) of PMA (50 ng/mL) and ionomycin (500 ng/mL) ( n = 4–10 independent values per group). * P < 0.05 vs. unstimulated; # P < 0.05 vs. corresponding vehicle; two-way ANOVA followed by Bonferroni’s post-test.

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: Effects of EphB4 on splenic mononuclear cells activation. TNFα production by cultured splenic mononuclear cells incubated with vehicle (Veh), cyclosporine A 1 µg/ml (CsA) or EphB4 (10–100 ng/ml) (EphB4) in the presence (black bars) or absence (white bars) of PMA (50 ng/mL) and ionomycin (500 ng/mL) ( n = 4–10 independent values per group). * P < 0.05 vs. unstimulated; # P < 0.05 vs. corresponding vehicle; two-way ANOVA followed by Bonferroni’s post-test.

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques: Activation Assay, Cell Culture, Incubation

Binding affinity of monomeric EphB4 to ephrin-B1 and ephrin-B2. EphB4 concentration-dependently displaced the binding of biotinylated ephrin-B1-Fc from immobilized EphB1-Fc (white circles) or the binding of biotinylated ephrin-B2-Fc from immobilized EphB4-Fc (black circles) ( n = 3 independent experiments).

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: Binding affinity of monomeric EphB4 to ephrin-B1 and ephrin-B2. EphB4 concentration-dependently displaced the binding of biotinylated ephrin-B1-Fc from immobilized EphB1-Fc (white circles) or the binding of biotinylated ephrin-B2-Fc from immobilized EphB4-Fc (black circles) ( n = 3 independent experiments).

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques: Binding Assay, Concentration Assay

Graphical summary. The scheme describes the main findings of the manuscript and represents the ability of monomeric protein EphB4 to counteract the TNBS-induced inflammatory responses by interfering with EphB–ephrin-B interaction.

Journal: Frontiers in Pharmacology

Article Title: Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD

doi: 10.3389/fphar.2019.00691

Figure Lengend Snippet: Graphical summary. The scheme describes the main findings of the manuscript and represents the ability of monomeric protein EphB4 to counteract the TNBS-induced inflammatory responses by interfering with EphB–ephrin-B interaction.

Article Snippet: EphB4 (His Tag) (Sino Biological Inc., Beijing, China 50582-M08H) was first incubated at 1 to 10,000 ng/ml with biotinylated ephrin-B1-Fc or ephrin-B2-Fc for 30 min at room temperature and then added to the wells at 37°C for 1 h. Finally, wells were washed and incubated with 100 µL/well Streptavidin-HRP (Sigma-Aldrich, Milan, Italy, S5512) for 20 min at room temperature, washed again and incubated at room temperature with 100 µl/well 0.1 g/L tetra-methylbenzidine (Sigma-Aldrich, Milan, Italy, 860336) reconstituted in stable peroxide buffer (11.3 g/L citric acid, 9.7 g/L sodium phosphate, pH 5.0), and 0.02% H 2 O 2 (30% w/w in water), added immediately before use.

Techniques: