Journal: Frontiers in Physiology

Article Title: β-Cyclodextrins Decrease Cholesterol Release and ABC-Associated Transporter Expression in Smooth Muscle Cells and Aortic Endothelial Cells

doi: 10.3389/fphys.2016.00185

Figure Lengend Snippet: Cholesterol efflux from ABAE and SMCs . Cells were first labeled with [ 3 H]-cholesterol (0.5 μCi/mL) for 36 h at 37°C and then equilibrated for 24 h in DMEM/0.05% BSA at 37°C. Cellular [ 3 H]-cholesterol loads in DPM per μg of proteins were compared between ABAE and SMCs (A) . Data represent the mean ± SEM (in DPM/μg of proteins), with n = 6 from two sets of experiments. Paired- t -test was performed, *** p < 0.001 when compared with ABAE condition. [ 3 H]-cholesterol released from both cell types was then measured 6 h after medium supplementation with either BSA (control condition) or cholesterol acceptors [ApoA-I (20 μg/mL) or HDL (50 μg/mL)] at 37°C (B) . Data represent the mean ± SEM (in DPM/μg of proteins), with n = 6 from two sets of experiments. One-way ANOVA test followed by Bonferroni correction was performed in which *** p < 0.001 refers to the control condition and ### p < 0.001 refers to the matched HDL condition in ABAE.

Article Snippet: ApoA-I and HDL were purchased from PROSCI Incorporated (Poway, CA, USA).

Techniques: Labeling, Control

Journal: Frontiers in Physiology

Article Title: β-Cyclodextrins Decrease Cholesterol Release and ABC-Associated Transporter Expression in Smooth Muscle Cells and Aortic Endothelial Cells

doi: 10.3389/fphys.2016.00185

Figure Lengend Snippet: Cholesterol release from ABAE (A,C) and from SMCs (B,D) upon CD-treatment . Cells were first labeled with [ 3 H]-cholesterol (0.5 μCi/mL) for 36 h at 37°C and then equilibrated in DMEM/0.05% BSA for 24 h at 37°C. [ 3 H]-cholesterol released in the medium from both cell types was then measured 6 h after medium supplementation with cholesterol acceptors [ApoA-I (20 μg/mL) or HDL (50 μg/mL)] at 37°C. The radioactivity contained in the culture medium, that corresponds to the cholesterol released from cells was measured. Data are expressed as the mean ± SEM (in DPM/μg of proteins, n = 6 from two sets of experiments). Statistical analysis: a one-way ANOVA followed by Dunnett's test for multiple comparisons in which * p < 0.05; *** p < 0.001 compared with the control condition (open bars) without any CD treatment.

Article Snippet: ApoA-I and HDL were purchased from PROSCI Incorporated (Poway, CA, USA).

Techniques: Labeling, Radioactivity, Control

Journal: Frontiers in Physiology

Article Title: β-Cyclodextrins Decrease Cholesterol Release and ABC-Associated Transporter Expression in Smooth Muscle Cells and Aortic Endothelial Cells

doi: 10.3389/fphys.2016.00185

Figure Lengend Snippet: Effect of 10 μM T0901317 on cholesterol efflux to ApoA-I and HDL in ABAE (A) and SMCs (B) treated or nor with Rameβ . Cells were first labeled with [ 3 H]-cholesterol as previously described and incubated in the presence of 1 mM Rameβ, 10 μM T0901317 or both during 24 h. Then, [ 3 H]-cholesterol released in the medium from both cell types was measured 6 h after medium supplementation with either ApoA-I (20 μg/mL) or HDL (50 μg/mL). Data are expressed as the mean ± SEM (in DPM/μg of proteins, n = 6 from two sets of experiments). Control condition (without any treatment) is used as the reference level and set to 100%. Statistical analysis: a one-way ANOVA followed by Dunnett's test for multiple comparisons in which * p < 0.05; ** p < 0.01; *** p < 0.001 compared with the untreated condition (Control condition, open bars) and in which ## p < 0.01 when Rameβ + T0901317 condition was compared with Rameβ condition.

Article Snippet: ApoA-I and HDL were purchased from PROSCI Incorporated (Poway, CA, USA).

Techniques: Labeling, Incubation, Control