Journal: The ISME Journal

Article Title: Induction of antibacterial proteins and peptides in the coprophilous mushroom Coprinopsis cinerea in response to bacteria

doi: 10.1038/s41396-018-0293-8

Figure Lengend Snippet: Activity of C. cinerea GH24-type lysozymes on Gram-positive bacteria. a – f Bacterial cell lysis activity was determined in a microtiter plate turbidity assay for B. subtilis , M. luteus , S. carnosus and S. aureus . Cells were treated with the following proteins at a concentration of 20 µg/ml: Untagged recombinant lysozymes (LYS1-3), His6-tagged LYS2 (LYS2His), His6-tagged lysozyme domain of LYS2 (LYS2His∆N), His6-tagged catalytic mutant LYS2 (LYS2(D131A)His), hen egg white lysozyme (HEWL) as positive and bovine serum albumin (BSA) as negative control. The bacterial suspensions were incubated at 37 °C and data points were acquired at an optical density of 450 nm in 5 min time intervals. The average of three biological replicates is shown together with the standard deviation. g Lysozyme activity was determined using the EnzChek Lysozyme assay kit (Thermo Scientific). 4-fold dilution series of the proteins listed above were incubated with M. luteus fluorescein-labeled cell wall. The release of fluorescein due to hydrolysis of cell wall was measured after 30 min of incubation at 37 °C with excitation and emission wavelengths of 485 nm and 535 nm, respectively. Each data point represents the average of three replicates. Error bars indicate standard deviation

Article Snippet: Micrococcus luteus (DSMZ 20030) was cultivated in nutrient broth (Difco, BD Diagnostics) at 28 °C to an OD 600 of 2.

Techniques: Activity Assay, Bacteria, Lysis, Concentration Assay, Recombinant, Mutagenesis, Negative Control, Incubation, Standard Deviation, Labeling

Journal: Microorganisms

Article Title: In Vitro Efficacy of Isobutyl Cyanoacrylate Nanoparticles against Fish Bacterial Pathogens and Selection Preference by Rainbow Trout ( Oncorhynchus mykiss )

doi: 10.3390/microorganisms11122877

Figure Lengend Snippet: Percent growth inhibition (GI %) of Vibrio spp. at all growth inhibiting concentrations: ( a ) GI % of Vibrio anguillarum for D60 (I) and NP30 (II); ( b ) GI % of Vibrio harveyi for D60 (I) and NP30 (II); ( c ) GI % of Vibrio parahaemolyticus for D60 (I) and NP30 (II); ( d ) GI % of Vibrio rotiferanus for D60 (I) and NP30 (II). All data are expressed as means ± SEM ( n = 3).

Article Snippet: In the case of D60, dextran 60,000 (041-30525; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was the dispersant, with a final concentration of 1.10% ( w / v ).

Techniques: Inhibition

Journal: Microorganisms

Article Title: In Vitro Efficacy of Isobutyl Cyanoacrylate Nanoparticles against Fish Bacterial Pathogens and Selection Preference by Rainbow Trout ( Oncorhynchus mykiss )

doi: 10.3390/microorganisms11122877

Figure Lengend Snippet: Percent growth inhibition (GI %) of some Gram-positive bacteria strains at all growth-inhibiting concentrations observed: ( a ) GI % of Lactococcus garvieae ; ( b ) GI % of Nocardia seriolae ; ( c ) GI % of Streptococcus iniae for D60 (I) and NP30 (II). All data are expressed as means ± SEM ( n = 3).

Article Snippet: In the case of D60, dextran 60,000 (041-30525; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was the dispersant, with a final concentration of 1.10% ( w / v ).

Techniques: Inhibition, Bacteria

Journal: Microorganisms

Article Title: In Vitro Efficacy of Isobutyl Cyanoacrylate Nanoparticles against Fish Bacterial Pathogens and Selection Preference by Rainbow Trout ( Oncorhynchus mykiss )

doi: 10.3390/microorganisms11122877

Figure Lengend Snippet: Percent growth inhibition (GI %) of some Gram-negative bacteria strains at all growth-inhibiting concentrations observed: ( a ) GI % of Photobacterium damselae subsp. piscicida ; ( b ) GI % of Tericibaculum maritimum ; ( c ) GI % of Aeromonas. salmonicida subsp. salmonicida ; ( d ) GI % of Edwardsiella tarda (typical); ( e ) GI % of Edwardsiella tarda (atypical) for D60 (I) and NP30 (II). All data are expressed as means ± SEM ( n = 3).

Article Snippet: In the case of D60, dextran 60,000 (041-30525; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was the dispersant, with a final concentration of 1.10% ( w / v ).

Techniques: Inhibition, Bacteria

Journal: Microorganisms

Article Title: In Vitro Efficacy of Isobutyl Cyanoacrylate Nanoparticles against Fish Bacterial Pathogens and Selection Preference by Rainbow Trout ( Oncorhynchus mykiss )

doi: 10.3390/microorganisms11122877

Figure Lengend Snippet: Antibacterial activity of isobutyl cyanoacrylate nanoparticles, D60 (150–180 nm) and NP30 (10–50 nm) expressed as MIC and MBC against some fish bacterial pathogens.

Article Snippet: In the case of D60, dextran 60,000 (041-30525; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was the dispersant, with a final concentration of 1.10% ( w / v ).

Techniques: Activity Assay, Bacteria

Journal: Microorganisms

Article Title: In Vitro Efficacy of Isobutyl Cyanoacrylate Nanoparticles against Fish Bacterial Pathogens and Selection Preference by Rainbow Trout ( Oncorhynchus mykiss )

doi: 10.3390/microorganisms11122877

Figure Lengend Snippet: Overall bacterial sensitivity via percent growth inhibition by ( A ) D60 and ( B ) NP30 at 0.5 mg/mL in descending order.

Article Snippet: In the case of D60, dextran 60,000 (041-30525; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was the dispersant, with a final concentration of 1.10% ( w / v ).

Techniques: Inhibition

Journal: Microorganisms

Article Title: In Vitro Efficacy of Isobutyl Cyanoacrylate Nanoparticles against Fish Bacterial Pathogens and Selection Preference by Rainbow Trout ( Oncorhynchus mykiss )

doi: 10.3390/microorganisms11122877

Figure Lengend Snippet: Growth performance, feed utilization, and health conditions of rainbow trout fed the test diets (with iBCA-NP—D60, NP30, or without any (control)).

Article Snippet: In the case of D60, dextran 60,000 (041-30525; FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) was the dispersant, with a final concentration of 1.10% ( w / v ).

Techniques: Control