Journal: PLoS Pathogens

Article Title: An Epigenetic Compound Library Screen Identifies BET Inhibitors That Promote HSV-1 and -2 Replication by Bridging P-TEFb to Viral Gene Promoters through BRD4

doi: 10.1371/journal.ppat.1005950

Figure Lengend Snippet: (A) Titration of infectious virus production by plaque assay. Vero cells in triplicate were treated with a test compound or with 0.1% DMSO (Mock) at 2 hr prior to inoculation. HSV-1 or HSV-2 at 1 pfu/cell (MOI = 1) was used. The compounds were left throughout the infection. The samples were harvested at 24 hr PI and used to titrate infectious virus by plaque assay. The concentrations are as the following: JQ1 at 300 nM, PFI-1 at 500 nM, I-BET-762 (I-BET) at 1 μM, TG101348 (TG) at 3 μM, and TSA at 150 nM. (B) Representative compounds JQ1 and PFI-1 on plaque sizes. Vero cells in 6-well plates were infected in the presence or absence of a test compound with approximately 50–100 pfu/well of HSV-1 or HSV-2. Wells from plaque assay were photographed and the sizes of 30 randomly selected plaques were measured using ImageJ. The relative sizes the plaques were plotted and presented as mean ± SD. Data were analyzed using paired T test for statistical significance. * denotes p<0.05, and ** as p<0.01.

Article Snippet: Trichostatin A (TSA), PFI-1, JQ1, TG101348, RVX-208, and LDC000067 (CDK9 inhibitor) were also purchased from Selleck Chemicals. (-)-JQ1 was purchased from MedChem Express.

Techniques: Titration, Virus, Plaque Assay, Infection

Journal: Cell chemical biology

Article Title: Discovery of Druggable Host Factors Critical to Plasmodium Liver-Stage Infection

doi: 10.1016/j.chembiol.2019.05.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Plasmid: pHAHA , Luu et al., 2005 , Addgene Plasmid #12517.

Techniques: Virus, Infection, Recombinant, Functional Assay, Luciferase, Viability Assay, SYBR Green Assay, Reverse Transcription, Construct, Plasmid Preparation, Software