13468-H08H Search Results


93
Sino Biological human rnase 1
Total cellular RNA was prepared from EAhy926 and blood cells, and RT-PCR was performed as described in Materials and Methods. Densitometric analysis of the gels were used to represent the mRNA ratios by using Scion Image PC (National Institutes of Health). Results were normalized to the expression levels (E) of GAPDH and expressed as the ratio E (target)/E (GAPDH) (target = <t>RNase</t> <t>1</t> or RI). Each value represents the mean ± SD (n = 6) compared with EAhy926 cells. * indicates a significant differences with p<0.05, whereas ** indicates a significant difference with p<0.01.
Human Rnase 1, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/13468-H08H/pmc05362223-41-1-11?v=Sino+Biological
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human rnase 1 - by Bioz Stars, 2026-08
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Total cellular RNA was prepared from EAhy926 and blood cells, and RT-PCR was performed as described in Materials and Methods. Densitometric analysis of the gels were used to represent the mRNA ratios by using Scion Image PC (National Institutes of Health). Results were normalized to the expression levels (E) of GAPDH and expressed as the ratio E (target)/E (GAPDH) (target = RNase 1 or RI). Each value represents the mean ± SD (n = 6) compared with EAhy926 cells. * indicates a significant differences with p<0.05, whereas ** indicates a significant difference with p<0.01.

Journal: PLoS ONE

Article Title: The expression and localization of RNase and RNase inhibitor in blood cells and vascular endothelial cells in homeostasis of the vascular system

doi: 10.1371/journal.pone.0174237

Figure Lengend Snippet: Total cellular RNA was prepared from EAhy926 and blood cells, and RT-PCR was performed as described in Materials and Methods. Densitometric analysis of the gels were used to represent the mRNA ratios by using Scion Image PC (National Institutes of Health). Results were normalized to the expression levels (E) of GAPDH and expressed as the ratio E (target)/E (GAPDH) (target = RNase 1 or RI). Each value represents the mean ± SD (n = 6) compared with EAhy926 cells. * indicates a significant differences with p<0.05, whereas ** indicates a significant difference with p<0.01.

Article Snippet: Purified human RNase 1 (13468-H08H) and RI protein (M0307S) were from Sino Biologicals (Peking, PR China) and New England Biolabs Japan (Tokyo, Japan), respectively.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

(A, B) Supernatants from the different cell types were prepared and analyzed by Western blot as described in Materials and Methods with antibodies against RNase 1 (poly) and RI (mono). Lane 1. positive control (A: 10 ng (0.56 pmol) of RNase 1, B: 30 ng (0.61 pmol) of RI), 2. EAhy926 cells, 3. platelets, 4. MNCs, 5. PMNs, 6. RBCs.

Journal: PLoS ONE

Article Title: The expression and localization of RNase and RNase inhibitor in blood cells and vascular endothelial cells in homeostasis of the vascular system

doi: 10.1371/journal.pone.0174237

Figure Lengend Snippet: (A, B) Supernatants from the different cell types were prepared and analyzed by Western blot as described in Materials and Methods with antibodies against RNase 1 (poly) and RI (mono). Lane 1. positive control (A: 10 ng (0.56 pmol) of RNase 1, B: 30 ng (0.61 pmol) of RI), 2. EAhy926 cells, 3. platelets, 4. MNCs, 5. PMNs, 6. RBCs.

Article Snippet: Purified human RNase 1 (13468-H08H) and RI protein (M0307S) were from Sino Biologicals (Peking, PR China) and New England Biolabs Japan (Tokyo, Japan), respectively.

Techniques: Western Blot, Positive Control

After fixation and blocking, cells were stained with antibodies against RNase 1 (green), RI (red), and nuclei (blue). Each image of platelets-2 and thrombin-activated platelets-2 is optical micrograph of platelets-1 and thrombin-activated platelets-1. Scale bars = 10 μm.

Journal: PLoS ONE

Article Title: The expression and localization of RNase and RNase inhibitor in blood cells and vascular endothelial cells in homeostasis of the vascular system

doi: 10.1371/journal.pone.0174237

Figure Lengend Snippet: After fixation and blocking, cells were stained with antibodies against RNase 1 (green), RI (red), and nuclei (blue). Each image of platelets-2 and thrombin-activated platelets-2 is optical micrograph of platelets-1 and thrombin-activated platelets-1. Scale bars = 10 μm.

Article Snippet: Purified human RNase 1 (13468-H08H) and RI protein (M0307S) were from Sino Biologicals (Peking, PR China) and New England Biolabs Japan (Tokyo, Japan), respectively.

Techniques: Blocking Assay, Staining

After fixation and blocking, cells were stained with antibodies against RNase 1 (green), VWF (red), and nuclei (blue). Scale bars = 10 μm.

Journal: PLoS ONE

Article Title: The expression and localization of RNase and RNase inhibitor in blood cells and vascular endothelial cells in homeostasis of the vascular system

doi: 10.1371/journal.pone.0174237

Figure Lengend Snippet: After fixation and blocking, cells were stained with antibodies against RNase 1 (green), VWF (red), and nuclei (blue). Scale bars = 10 μm.

Article Snippet: Purified human RNase 1 (13468-H08H) and RI protein (M0307S) were from Sino Biologicals (Peking, PR China) and New England Biolabs Japan (Tokyo, Japan), respectively.

Techniques: Blocking Assay, Staining

EAhy926 cells (A) or platelets (C) are TEM images. EAhy926 cells (B) or platelets (D) immunostained with anti-RNase 1 (arrows) and anti-VWF antibodies (arrowheads). Scale bars (A) 2 μm, (B) 100 nm, (C) 500 nm, (D) 100 nm.

Journal: PLoS ONE

Article Title: The expression and localization of RNase and RNase inhibitor in blood cells and vascular endothelial cells in homeostasis of the vascular system

doi: 10.1371/journal.pone.0174237

Figure Lengend Snippet: EAhy926 cells (A) or platelets (C) are TEM images. EAhy926 cells (B) or platelets (D) immunostained with anti-RNase 1 (arrows) and anti-VWF antibodies (arrowheads). Scale bars (A) 2 μm, (B) 100 nm, (C) 500 nm, (D) 100 nm.

Article Snippet: Purified human RNase 1 (13468-H08H) and RI protein (M0307S) were from Sino Biologicals (Peking, PR China) and New England Biolabs Japan (Tokyo, Japan), respectively.

Techniques:

EAhy926 cells (A, B) or platelets (C, D) immunostained with anti-RNase 1 (arrows, (A, C)) or anti-VWF antibodies (arrowheads, (B, D)). Scale bars = 500 nm.

Journal: PLoS ONE

Article Title: The expression and localization of RNase and RNase inhibitor in blood cells and vascular endothelial cells in homeostasis of the vascular system

doi: 10.1371/journal.pone.0174237

Figure Lengend Snippet: EAhy926 cells (A, B) or platelets (C, D) immunostained with anti-RNase 1 (arrows, (A, C)) or anti-VWF antibodies (arrowheads, (B, D)). Scale bars = 500 nm.

Article Snippet: Purified human RNase 1 (13468-H08H) and RI protein (M0307S) were from Sino Biologicals (Peking, PR China) and New England Biolabs Japan (Tokyo, Japan), respectively.

Techniques: