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All (9)
Santa Cruz
DSMZ
Cell Signaling
AstraZeneca
Othera Pharma
Verlag
Cayman
Addgene
1 More...
 

About 11 products / 53 snippets / 31 images

96 / 100
Bioz Stars
cathepsin D Antibody
Santa Cruz  
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Catalog#: SC-377299
Techniques
Western Blot, Incubation, Software, Staining, Immunofluorescence (see more)
Article Snippets
".. Cruz Biotechnology), anti-IL1β AF-401-NA (R & D Systems), or anti-cathepsin D C-20 (Santa Cruz Biotechnology) and peroxidase-conjugated secondary antibodies."  (see more)
94 / 100
Bioz Stars
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Rabbit mAb
Cell Signaling Technology  
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Catalog#: 13148
Techniques
Recombinant, Staining, Sulforhodamine B Assay, Luciferase, Flow Cytometry (see more)
Article Snippets
"Rabbit monoclonal phospho-p44/42 MAPK (Erk1⁄2) (Thr202/Tyr204) (197G2) , Cell Signaling Technology , Cat# 13148."  (see more)
FLAG-T
RECOMB
TNSF
SOFT
27+  more images
(A) Lysosomes are recruited to parasite binding site during L. amazonensis invasion. MEFs were incubated with LLa -RFP for 30 min, permeabilized and labeled to visualize lysosomes (green) and nuclei (blue), followed by fluorescence microscopy - white arrows indicate parasites interacting with the host cell (bright field) and attracting host cell lysosomes. (B) Same as A but cells were not permeabilized to visualize only the exposure of lysosomal luminal epitopes on the plasma membrane. (C) Quantification of lysosomal epitope exposure on the surface of MEFs incubated with live (blue) or PFA-fixed L. amazonensis (green) removed from the dish by scraping and analyzed by FACS. (D to E) Exocytosis of host cell lysosomal enzymes during L. amazonensis invasion in MEFs. (C) The activity of beta-Hex was assayed in supernatants of cells incubated with living (solid line) and PFA-fixed L. amazonensis (dashed line). Controls with L. amazonensis alone were carried out (dotted line). (E) Supernatants were analyzed by western blotting using anti-ASM or anti-cathepsin D (Cat-D) antibodies. (F) Endocytosis quantification in MEFs incubated with LLa- RFP. MEF PM was labeled with A-488-conjugated WGA before incubation with parasites for 15 min. After parasite removal the extracellular fluorescence was quenched by trypan blue and the endocytosed dye was quantified by FACS. (G to H) Detection of lysosomal markers, ceramide and LAMP recently formed L. amazonensis vacuoles. MEFs were infected with LLa -RFP for 1 h and labeled to visualize lysosomes (green) (G), ceramide (green) (H), DAPI (blue) to stain nuclei, and imaged by fluorescence microscopy.
BIND-A
INCU
LBLNG
FLUOR
MICRO
KEY RESOURCES TABLE
FLAG-T
RECOMB
TNSF
SOFT
List of antibodies used in this study
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92 / 100
Bioz Stars
Geobacillus thermodenitrificans
DSMZ  
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Catalog#: DSM 465
Techniques
Isolation, Incubation, Sequencing, Amplification, Purification (see more)
Article Snippets
".. 1321), Bacillus thermodenitrificans (DSM 465), and Bacillus azotoformans (DSM 1046) were obtained from the Deutsche Sammlung von Mikroroganismen und Zellkulturen GmbH, Braunschweig, .."  (see more)
86 / 100
Bioz Stars
Industry funding 13,148 (50.4) 12,364 (57.4) 11,798
Othera Pharmaceuticals Inc  
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Techniques
 
Article Snippets
".. primary, secondary, or tertiary sponsor) 6,582 (25.2) 4,957 (23.0) 3,882 (21.0) Any industry funding 13,148 (50.4) 12,364 (57.4) 11,798 (63.9) Othera sole sponsor 6,350 (24.3) 4,237 (19.7) 2,769 (15.0) Purpose Basic science 295 .."  (see more)
86 / 100
Bioz Stars
Anti Nasal polyp Cathepsin D Santa Cruz Sc-13148 Mouse
Santa Cruz  
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Techniques
 
Article Snippets
".. 1:100 Nasal polyp Cathepsin B Santa Cruz Sc-365558 Mouse 1:100 Nasal polyp Cathepsin D Santa Cruz Sc-13148 Mouse 1:200 Nasal polyp Beclin-1 Novus Bio NB500-249 Rabbit 1:800 Nasal polyp .."  (see more)
86 / 100
Bioz Stars
Angewandte Zuschriften 13148
Verlag  
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Techniques
 
Article Snippets
"Abstract: Die Entwicklung von Rezeptor-TyrosinkinaseInhibitoren (TKIs) ist ein Meilenstein in der Entwicklung neuer Tumortherapeutika.. Allerdings geht die TKI-Therapie mit starken Nebenwirkungen und Chemotherapieresistenz einher.. Ziel diese ..."  (see more)
86 / 100
Bioz Stars
I/II Keryx AT-13148 Phase I AstraZeneca & Astex MK-2206 Phase
AstraZeneca  
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Techniques
 
Article Snippets
"Abstract: Epidemiologic and experimental studies support a key role of the phosphatidyl inositol 3-kinase/AKT/ mammalian target of rapamycin (PI3K/AKT/mTOR) pathway in the biology of human cancers.. Alterations resulting in activation of PI3K ..."  (see more)
90 / 100
Bioz vStars
at 13148
Cayman  
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Catalog#: 21597
Description
"AT 13148 is an orally bioavailable and ATP competitive multi AGC kinase inhibitor It inhibits Akt1 2 and 3 ICs 38 402 and 50 nM respectively in addition to other AGC kinase family members p70S6K"  (see more)
90 / 100
Bioz vStars
at 13148
Cayman  
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Catalog#: 21597-1MG
Description
"AT 13148"
93 / 100
Bioz vStars
AT 13148
Biosynth  
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Catalog#: FA137648
Description
"Inhibitor of ROCK I and ROCK II kinases; multi-AGC kinase inhibitor. Inhibitor of ROCK I and ROCK II kinases; multi-AGC kinase inhibitor."
90 / 100
Bioz vStars
pc3xb zf49 plasmid 13148
Addgene  
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Catalog#: 13148
Description
"Standard format Plasmid sent in bacteria as agar stab"