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Image Search Results
Journal: Developmental cell
Article Title: Akt regulates a Rab11-effector switch required for ciliogenesis
doi: 10.1016/j.devcel.2019.05.022
Figure Lengend Snippet: (A) Immunoblot showing co-immunoprecipitation of GFP-Rab11a Q70L with HA-Rabin8 in the presence of increasing amounts of WDR44 transiently-expressed in 293T cells grown in serum for 48h. Immunoblots were probed with GFP, HA, and WDR44 antibodies. Representative blot from two independent experiments is shown.
Article Snippet: Antibodies Commercial antibodies were used for acetylated a-tubulin ( Ac tub) (Catalog no. T7451, Sigma, RRID:AB_609894), pericentrin (Catalog no. NB100-61071, Novus, RRID:AB_925559), CEP164 (Catalog no. N-40 SC240226, SantaCruz, RRID:AB_10841981), anti-gamma tubulin (Catalog no. T6557, Sigma), CP110 (Catalog no., 12780-1-AP, Proteintech, RRID:AB_10638480), LPAR1/EDG2 (Catalog no. PA5-28768, ThermoFisher, RRID:AB_2546244), tRFP (Catalog no. AB233, Evrogen, RRID:AB_2571743), GFP for IP (custom), GFP-HRP (Catalog no. 130-091-833, Miltenyi Biotec, RRID:AB_247003), Rab11 (Catalog no. 610657, BD Biosciences, RRID:AB_397984), Phospho Akt substrate (RXRXXS*/T*) 23C8D2 (Catalog no. 10001S, Cell Signaling, RRID:AB_10950819), Phospho-AKT S473 (Catalog no. 4060S, Cell Signaling, RRID:AB_2315049), Akt pan C67E7 (Catalog no. 4691S, Cell Signaling, RRID:AB_915783), HA-HRP (Catalog no. 12013819001, Roche/Sigma, RRID:AB_390917), WDR44 for IP and Western (Catalog no. A301-440A, Bethyl, RRID:AB_961125), β-Actin-HRP (Catalog no. A3854, Sigma-Aldrich, RRID:AB_262011),
Techniques: Western Blot, Immunoprecipitation
Journal: Developmental cell
Article Title: Akt regulates a Rab11-effector switch required for ciliogenesis
doi: 10.1016/j.devcel.2019.05.022
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Antibodies Commercial antibodies were used for acetylated a-tubulin ( Ac tub) (Catalog no. T7451, Sigma, RRID:AB_609894), pericentrin (Catalog no. NB100-61071, Novus, RRID:AB_925559), CEP164 (Catalog no. N-40 SC240226, SantaCruz, RRID:AB_10841981), anti-gamma tubulin (Catalog no. T6557, Sigma), CP110 (Catalog no., 12780-1-AP, Proteintech, RRID:AB_10638480), LPAR1/EDG2 (Catalog no. PA5-28768, ThermoFisher, RRID:AB_2546244), tRFP (Catalog no. AB233, Evrogen, RRID:AB_2571743), GFP for IP (custom), GFP-HRP (Catalog no. 130-091-833, Miltenyi Biotec, RRID:AB_247003), Rab11 (Catalog no. 610657, BD Biosciences, RRID:AB_397984), Phospho Akt substrate (RXRXXS*/T*) 23C8D2 (Catalog no. 10001S, Cell Signaling, RRID:AB_10950819), Phospho-AKT S473 (Catalog no. 4060S, Cell Signaling, RRID:AB_2315049), Akt pan C67E7 (Catalog no. 4691S, Cell Signaling, RRID:AB_915783), HA-HRP (Catalog no. 12013819001, Roche/Sigma, RRID:AB_390917), WDR44 for IP and Western (Catalog no. A301-440A, Bethyl, RRID:AB_961125), β-Actin-HRP (Catalog no. A3854, Sigma-Aldrich, RRID:AB_262011),
Techniques: Immunofluorescence, Recombinant, Sequencing, Software