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Image Search Results
Journal: Gut microbes
Article Title: CD44 is a macrophage receptor for TcdB from Clostridioides difficile that via its lysine-158 succinylation contributes to inflammation.
doi: 10.1080/19490976.2025.2506192
Figure Lengend Snippet: Figure 3. CD44 functions as an independent receptor for TcdB/FBD in macrophages. (a – c) expression of known receptors (FZD1/2/7, CSPG4, PVRL3) in WT versus CD44-KO macrophages analyzed by (a) immunoblotting and (b, c) q-PCR. (d – g) competitive binding assays: (d, e) FZD1/2/7, CSPG4, and PVRL3 did not inhibit CD44 binding of (d) TcdB or (e) FBD, while (f, g) CD44 similarly did not interfere with TcdB/FBD binding to other receptors. (ELISA protocol: wells coated with 1 μM target protein [CD44-ECD or other receptors] were incubated with 1 μM TcdB/FBD, followed by competitor proteins. Binding was quantified by absorbance measure ment). (h – k) IL-1β (h, i) and IL-6 (j, k) levels in THP-1-Mφ treated with TcdB/FBD (100 pM) and receptor proteins (1 μM) for 24 h. All data in parts (b – k) are shown as the mean ± SEM (n = 3). ((ns: p > 0.05, **p < 0.01, &p < 0.001, #p < 0.0001).
Article Snippet: The other Primary antibodies used in this study were as follows: (Cat# 22915–1-AP), CSPG4 (Cat# 55027–1-AP),
Techniques: Expressing, Western Blot, Binding Assay, Enzyme-linked Immunosorbent Assay, Incubation