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    • allin rph  (highQu Inc)
    93
    highQu Inc allin rph
    Allin Rph, supplied by highQu Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allin rph/product/highQu Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    allin rph - by Bioz Stars, 2023-03
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    pcr water  (highQu Inc)
    93
    highQu Inc pcr water
    Comparison of NGAL expression between M-1 wild type and 2L3 ADPKD cells. ( A ) Structure of wild type and L3 alleles of Pkd1 gene. <t>PCR</t> genotyping was conducted using primers before and after the first loxP within the intron 30 of Pkd1. Filled triangles: loxP; an mc1 promoter-driven neomycin resistance gene (mc1-neo) flanked by two loxP sites was inserted into intron 34 of Pkd1. ( B ) PCR genotyping of Pkd1 transcripts of M-1 cells (473 bp) and 2L3 cells (507 bp). Pkd1 transcripts of the wild type (+/+), heterozygous (L3/+), and homozygous (L3/L3) mice were used as the <t>control.</t> <t>RT–qPCR</t> showed that 2L3 had significantly lower Pkd1 ( C ) M-1 and 2L3 cells show similar morphology. Scale bar: 100 μm. ( D ) M-1 and 2L3 cells were stained with Lotus Tetragonolobus Lectin (LTL, labeled with Fluorescein, green) and Dolichos biflorus agglutinin (DBA, labeled with Rhodamine, red) and DAPI (blue) and observed by a confocal microscope. Scale bar: 50 μm. RT–qPCR showed that 2L3 had significantly lower Pkd1 ( E ) and higher Lcn2 (NGAL) ( F ) expression levels than that of M-1 wild type cells. ( G ) No significant difference in Slc22a17 (NGAL-R) expression levels between M-1 and 2L3 cells. RQ: relative quantification = 2 −ΔΔCt ; Gapdh was used as the internal control of each sample. Values were normalized to M-1. ( H ) Western blot results showed that 2L3 cells had significantly higher expression of NGAL but not of NGAL-R than M-1 cells. Actin and GAPDH were used as the internal controls of each sample. Values were normalized to the control group of 2L3. ( I ) ELISA showed that 2L3 had higher expression of NGAL compared with M-1 in both cell lysate and supernatant. Student’s t -test was performed to determine the significance between the two groups ( n = 6 in RT–qPCR, n = 3 in western blot and ELISA). * p < 0.05; *** p < 0.001 vs. M-1 of each group; ns: no significant difference.
    Pcr Water, supplied by highQu Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr water/product/highQu Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr water - by Bioz Stars, 2023-03
    93/100 stars
    		  Buy from Supplier
    allin red taq mastermix  (highQu Inc)
    93
    highQu Inc allin red taq mastermix
    Comparison of NGAL expression between M-1 wild type and 2L3 ADPKD cells. ( A ) Structure of wild type and L3 alleles of Pkd1 gene. <t>PCR</t> genotyping was conducted using primers before and after the first loxP within the intron 30 of Pkd1. Filled triangles: loxP; an mc1 promoter-driven neomycin resistance gene (mc1-neo) flanked by two loxP sites was inserted into intron 34 of Pkd1. ( B ) PCR genotyping of Pkd1 transcripts of M-1 cells (473 bp) and 2L3 cells (507 bp). Pkd1 transcripts of the wild type (+/+), heterozygous (L3/+), and homozygous (L3/L3) mice were used as the <t>control.</t> <t>RT–qPCR</t> showed that 2L3 had significantly lower Pkd1 ( C ) M-1 and 2L3 cells show similar morphology. Scale bar: 100 μm. ( D ) M-1 and 2L3 cells were stained with Lotus Tetragonolobus Lectin (LTL, labeled with Fluorescein, green) and Dolichos biflorus agglutinin (DBA, labeled with Rhodamine, red) and DAPI (blue) and observed by a confocal microscope. Scale bar: 50 μm. RT–qPCR showed that 2L3 had significantly lower Pkd1 ( E ) and higher Lcn2 (NGAL) ( F ) expression levels than that of M-1 wild type cells. ( G ) No significant difference in Slc22a17 (NGAL-R) expression levels between M-1 and 2L3 cells. RQ: relative quantification = 2 −ΔΔCt ; Gapdh was used as the internal control of each sample. Values were normalized to M-1. ( H ) Western blot results showed that 2L3 cells had significantly higher expression of NGAL but not of NGAL-R than M-1 cells. Actin and GAPDH were used as the internal controls of each sample. Values were normalized to the control group of 2L3. ( I ) ELISA showed that 2L3 had higher expression of NGAL compared with M-1 in both cell lysate and supernatant. Student’s t -test was performed to determine the significance between the two groups ( n = 6 in RT–qPCR, n = 3 in western blot and ELISA). * p < 0.05; *** p < 0.001 vs. M-1 of each group; ns: no significant difference.
    Allin Red Taq Mastermix, supplied by highQu Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allin red taq mastermix/product/highQu Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    allin red taq mastermix - by Bioz Stars, 2023-03
    93/100 stars
    		  Buy from Supplier
    cozyhi prestained protein ladder prl0202  (highQu Inc)
    88
    highQu Inc cozyhi prestained protein ladder prl0202
    Comparison of NGAL expression between M-1 wild type and 2L3 ADPKD cells. ( A ) Structure of wild type and L3 alleles of Pkd1 gene. <t>PCR</t> genotyping was conducted using primers before and after the first loxP within the intron 30 of Pkd1. Filled triangles: loxP; an mc1 promoter-driven neomycin resistance gene (mc1-neo) flanked by two loxP sites was inserted into intron 34 of Pkd1. ( B ) PCR genotyping of Pkd1 transcripts of M-1 cells (473 bp) and 2L3 cells (507 bp). Pkd1 transcripts of the wild type (+/+), heterozygous (L3/+), and homozygous (L3/L3) mice were used as the <t>control.</t> <t>RT–qPCR</t> showed that 2L3 had significantly lower Pkd1 ( C ) M-1 and 2L3 cells show similar morphology. Scale bar: 100 μm. ( D ) M-1 and 2L3 cells were stained with Lotus Tetragonolobus Lectin (LTL, labeled with Fluorescein, green) and Dolichos biflorus agglutinin (DBA, labeled with Rhodamine, red) and DAPI (blue) and observed by a confocal microscope. Scale bar: 50 μm. RT–qPCR showed that 2L3 had significantly lower Pkd1 ( E ) and higher Lcn2 (NGAL) ( F ) expression levels than that of M-1 wild type cells. ( G ) No significant difference in Slc22a17 (NGAL-R) expression levels between M-1 and 2L3 cells. RQ: relative quantification = 2 −ΔΔCt ; Gapdh was used as the internal control of each sample. Values were normalized to M-1. ( H ) Western blot results showed that 2L3 cells had significantly higher expression of NGAL but not of NGAL-R than M-1 cells. Actin and GAPDH were used as the internal controls of each sample. Values were normalized to the control group of 2L3. ( I ) ELISA showed that 2L3 had higher expression of NGAL compared with M-1 in both cell lysate and supernatant. Student’s t -test was performed to determine the significance between the two groups ( n = 6 in RT–qPCR, n = 3 in western blot and ELISA). * p < 0.05; *** p < 0.001 vs. M-1 of each group; ns: no significant difference.
    Cozyhi Prestained Protein Ladder Prl0202, supplied by highQu Inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cozyhi prestained protein ladder prl0202/product/highQu Inc
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cozyhi prestained protein ladder prl0202 - by Bioz Stars, 2023-03
    88/100 stars
    		  Buy from Supplier
    qscriber cdna synthesis kit  (highQu Inc)
    93
    highQu Inc qscriber cdna synthesis kit
    Comparison of NGAL expression between M-1 wild type and 2L3 ADPKD cells. ( A ) Structure of wild type and L3 alleles of Pkd1 gene. <t>PCR</t> genotyping was conducted using primers before and after the first loxP within the intron 30 of Pkd1. Filled triangles: loxP; an mc1 promoter-driven neomycin resistance gene (mc1-neo) flanked by two loxP sites was inserted into intron 34 of Pkd1. ( B ) PCR genotyping of Pkd1 transcripts of M-1 cells (473 bp) and 2L3 cells (507 bp). Pkd1 transcripts of the wild type (+/+), heterozygous (L3/+), and homozygous (L3/L3) mice were used as the <t>control.</t> <t>RT–qPCR</t> showed that 2L3 had significantly lower Pkd1 ( C ) M-1 and 2L3 cells show similar morphology. Scale bar: 100 μm. ( D ) M-1 and 2L3 cells were stained with Lotus Tetragonolobus Lectin (LTL, labeled with Fluorescein, green) and Dolichos biflorus agglutinin (DBA, labeled with Rhodamine, red) and DAPI (blue) and observed by a confocal microscope. Scale bar: 50 μm. RT–qPCR showed that 2L3 had significantly lower Pkd1 ( E ) and higher Lcn2 (NGAL) ( F ) expression levels than that of M-1 wild type cells. ( G ) No significant difference in Slc22a17 (NGAL-R) expression levels between M-1 and 2L3 cells. RQ: relative quantification = 2 −ΔΔCt ; Gapdh was used as the internal control of each sample. Values were normalized to M-1. ( H ) Western blot results showed that 2L3 cells had significantly higher expression of NGAL but not of NGAL-R than M-1 cells. Actin and GAPDH were used as the internal controls of each sample. Values were normalized to the control group of 2L3. ( I ) ELISA showed that 2L3 had higher expression of NGAL compared with M-1 in both cell lysate and supernatant. Student’s t -test was performed to determine the significance between the two groups ( n = 6 in RT–qPCR, n = 3 in western blot and ELISA). * p < 0.05; *** p < 0.001 vs. M-1 of each group; ns: no significant difference.
    Qscriber Cdna Synthesis Kit, supplied by highQu Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qscriber cdna synthesis kit/product/highQu Inc
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qscriber cdna synthesis kit - by Bioz Stars, 2023-03
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Comparison of NGAL expression between M-1 wild type and 2L3 ADPKD cells. ( A ) Structure of wild type and L3 alleles of Pkd1 gene. PCR genotyping was conducted using primers before and after the first loxP within the intron 30 of Pkd1. Filled triangles: loxP; an mc1 promoter-driven neomycin resistance gene (mc1-neo) flanked by two loxP sites was inserted into intron 34 of Pkd1. ( B ) PCR genotyping of Pkd1 transcripts of M-1 cells (473 bp) and 2L3 cells (507 bp). Pkd1 transcripts of the wild type (+/+), heterozygous (L3/+), and homozygous (L3/L3) mice were used as the control. RT–qPCR showed that 2L3 had significantly lower Pkd1 ( C ) M-1 and 2L3 cells show similar morphology. Scale bar: 100 μm. ( D ) M-1 and 2L3 cells were stained with Lotus Tetragonolobus Lectin (LTL, labeled with Fluorescein, green) and Dolichos biflorus agglutinin (DBA, labeled with Rhodamine, red) and DAPI (blue) and observed by a confocal microscope. Scale bar: 50 μm. RT–qPCR showed that 2L3 had significantly lower Pkd1 ( E ) and higher Lcn2 (NGAL) ( F ) expression levels than that of M-1 wild type cells. ( G ) No significant difference in Slc22a17 (NGAL-R) expression levels between M-1 and 2L3 cells. RQ: relative quantification = 2 −ΔΔCt ; Gapdh was used as the internal control of each sample. Values were normalized to M-1. ( H ) Western blot results showed that 2L3 cells had significantly higher expression of NGAL but not of NGAL-R than M-1 cells. Actin and GAPDH were used as the internal controls of each sample. Values were normalized to the control group of 2L3. ( I ) ELISA showed that 2L3 had higher expression of NGAL compared with M-1 in both cell lysate and supernatant. Student’s t -test was performed to determine the significance between the two groups ( n = 6 in RT–qPCR, n = 3 in western blot and ELISA). * p < 0.05; *** p < 0.001 vs. M-1 of each group; ns: no significant difference.

    Journal: Cells

    Article Title: Secreted Neutrophil Gelatinase-Associated Lipocalin Shows Stronger Ability to Inhibit Cyst Enlargement of ADPKD Cells Compared with Nonsecreted Form

    doi: 10.3390/cells11030483

    Figure Lengend Snippet: Comparison of NGAL expression between M-1 wild type and 2L3 ADPKD cells. ( A ) Structure of wild type and L3 alleles of Pkd1 gene. PCR genotyping was conducted using primers before and after the first loxP within the intron 30 of Pkd1. Filled triangles: loxP; an mc1 promoter-driven neomycin resistance gene (mc1-neo) flanked by two loxP sites was inserted into intron 34 of Pkd1. ( B ) PCR genotyping of Pkd1 transcripts of M-1 cells (473 bp) and 2L3 cells (507 bp). Pkd1 transcripts of the wild type (+/+), heterozygous (L3/+), and homozygous (L3/L3) mice were used as the control. RT–qPCR showed that 2L3 had significantly lower Pkd1 ( C ) M-1 and 2L3 cells show similar morphology. Scale bar: 100 μm. ( D ) M-1 and 2L3 cells were stained with Lotus Tetragonolobus Lectin (LTL, labeled with Fluorescein, green) and Dolichos biflorus agglutinin (DBA, labeled with Rhodamine, red) and DAPI (blue) and observed by a confocal microscope. Scale bar: 50 μm. RT–qPCR showed that 2L3 had significantly lower Pkd1 ( E ) and higher Lcn2 (NGAL) ( F ) expression levels than that of M-1 wild type cells. ( G ) No significant difference in Slc22a17 (NGAL-R) expression levels between M-1 and 2L3 cells. RQ: relative quantification = 2 −ΔΔCt ; Gapdh was used as the internal control of each sample. Values were normalized to M-1. ( H ) Western blot results showed that 2L3 cells had significantly higher expression of NGAL but not of NGAL-R than M-1 cells. Actin and GAPDH were used as the internal controls of each sample. Values were normalized to the control group of 2L3. ( I ) ELISA showed that 2L3 had higher expression of NGAL compared with M-1 in both cell lysate and supernatant. Student’s t -test was performed to determine the significance between the two groups ( n = 6 in RT–qPCR, n = 3 in western blot and ELISA). * p < 0.05; *** p < 0.001 vs. M-1 of each group; ns: no significant difference.

    Article Snippet: Each sample was prepared in a total volume of 20 μL, including gene-specific forward (100 nM), reverse (100 nM) primers , cDNA template (100 ng), ORA TM SEE qPCR Green ROX H Mix (1×, highQu GmbH, Kraichtal, Germany) and PCR water.

    Techniques: Expressing, Quantitative RT-PCR, Staining, Labeling, Microscopy, Western Blot, Enzyme-linked Immunosorbent Assay