Journal: Journal of Translational Medicine

Article Title: A translational approach for limb vascular delivery of the micro-dystrophin gene without high volume or high pressure for treatment of Duchenne muscular dystrophy

doi: 10.1186/1479-5876-5-45

Figure Lengend Snippet: Components of the dystrophin-associated protein complex are restored in mdx mice treated with rAAV.micro-dystrophin gene transfer. mdx mice treated with rAAV8.micro-dystrophin delivered by ILP (8 week time point) were serial sectioned and stained with manex1a antibody for dystrophin, α-sarcoglycan, β-sarcoglycan, and H &E. Muscle fibers transduced with micro-dystrophin also exhibited restoration of expression of α-sarcoglycan and β-sarcoglycan (no staining in untreated mdx mice – bottom row). Muscle fibers from serial sections exhibited identical expression localization for each protein (arrow).

Article Snippet: Cryostat sections (12 μm) for immunostains were incubated with the N-terminal Manex1a primary antibody (Developmental Studies Hybridoma Bank), alpha-sarcoglycan (Vector, VP-A108), or beta-sarcoglycan (Vector, VP-B206) primary antibody at a dilution of 1:50 in blocking buffer (PBS, 10% goat serum, 0.1% Triton x-100) for 1 hour at room temperature in a wet chamber.

Techniques: Staining, Transduction, Expressing