Journal: PLoS ONE

Article Title: STAT3 Activation in Skeletal Muscle Links Muscle Wasting and the Acute Phase Response in Cancer Cachexia

doi: 10.1371/journal.pone.0022538

Figure Lengend Snippet: A, Western blotting analysis and quantitation of fibrinogen in C2C12 myotubes infected with Ad-cSTAT3-GFP or Ad-GFP as control. Fibrinogen expression was increased consistent with the increase in the levels of STAT3. **P<0.01, ***P<0.001 vs. GFP. B, Western blotting analysis and quantitation of fibrinogen expression in C2C12 treated with IL-6 (100 ng/ml) for 1, 24, 48 h. GAPDH was used as loading control. Increased expression of fibrinogen was observed at each time point after IL-6 treatment. Data (means ± SEM) are expressed as relative densitometry value. ***P<0.001 vs. respective controls. C, Fibrinogen levels by ELISA of the conditioned medium of C2C12 exposed to IL-6 for 30 min, 1, 6, 24, 48 h. Fibrinogen levels were significantly elevated after 6, 24 and 48 h of IL-6 treatment. Data (means ± SEM) are expressed as ng/ml. **P<0.01, ***P<0.001 vs. controls (C).

Article Snippet: Fibrinogen levels were then assayed using the AssayMax Mouse Fibrinogen ELISA kit (AssayPro, St. Charles, MO, USA) following the instructions provided by the manufacturer.

Techniques: Western Blot, Quantitation Assay, Infection, Control, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of Visualized Experiments : JoVE

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

doi: 10.3791/3791

Figure Lengend Snippet:

Article Snippet: A16 , Mouse anti-human hemopexin monoclonal antibody , Hemopexin , Assaypro , 60190-05011.

Techniques: Plasmid Preparation, Positive Control