Journal: PLoS ONE

Article Title: STAT3 Activation in Skeletal Muscle Links Muscle Wasting and the Acute Phase Response in Cancer Cachexia

doi: 10.1371/journal.pone.0022538

Figure Lengend Snippet: A, Western blotting analysis and quantitation of fibrinogen in C2C12 myotubes infected with Ad-cSTAT3-GFP or Ad-GFP as control. Fibrinogen expression was increased consistent with the increase in the levels of STAT3. **P<0.01, ***P<0.001 vs. GFP. B, Western blotting analysis and quantitation of fibrinogen expression in C2C12 treated with IL-6 (100 ng/ml) for 1, 24, 48 h. GAPDH was used as loading control. Increased expression of fibrinogen was observed at each time point after IL-6 treatment. Data (means ± SEM) are expressed as relative densitometry value. ***P<0.001 vs. respective controls. C, Fibrinogen levels by ELISA of the conditioned medium of C2C12 exposed to IL-6 for 30 min, 1, 6, 24, 48 h. Fibrinogen levels were significantly elevated after 6, 24 and 48 h of IL-6 treatment. Data (means ± SEM) are expressed as ng/ml. **P<0.01, ***P<0.001 vs. controls (C).

Article Snippet: Fibrinogen levels were then assayed using the AssayMax Mouse Fibrinogen ELISA kit (AssayPro, St. Charles, MO, USA) following the instructions provided by the manufacturer.

Techniques: Western Blot, Quantitation Assay, Infection, Control, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of Visualized Experiments : JoVE

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

doi: 10.3791/3791

Figure Lengend Snippet:

Article Snippet: A16 , Mouse anti-human hemopexin monoclonal antibody , Hemopexin , Assaypro , 60190-05011.

Techniques: Plasmid Preparation, Positive Control

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Novel mutation in coagulation factor VII (Carmel mutation): Identification and characterization

doi: 10.1002/rth2.12407

Figure Lengend Snippet: Factor VII activity and antigenicity

Article Snippet: Factor VII antigen was determined using the AssayMax human factor VII ELISA kit (Assaypro, St. Charles, MO, USA).

Techniques: Activity Assay, Recombinant, Control

Journal: Research and Practice in Thrombosis and Haemostasis

Article Title: Novel mutation in coagulation factor VII (Carmel mutation): Identification and characterization

doi: 10.1002/rth2.12407

Figure Lengend Snippet: ConSeq evolutionary conservation analysis of Cys164 FVII protein. Prediction conservation of residues 141‐184 in the mature protein, colored according to evolutionary conservation score. Scale bar for the evolutionary conservation scores is displayed in the bottom. Position 164 marked in square demonstrate the missense mutation. The nature of the residues designated as detailed: e ‐ An exposed residue according to the neural‐network algorithm.b ‐ A buried residue according to the neural‐network algorithm.f ‐ A predicted functional residue (highly conserved and exposed).s ‐ A predicted structural residue (highly conserved and buried)

Article Snippet: Factor VII antigen was determined using the AssayMax human factor VII ELISA kit (Assaypro, St. Charles, MO, USA).

Techniques: Mutagenesis, Residue, Functional Assay