Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Dual-targeted nanoparticulate drug delivery systems for enhancing triple-negative breast cancer treatment.

doi: 10.1016/j.jconrel.2024.06.012

Figure Lengend Snippet: Fig. 3. BRD4 inhibition exacerbates DSBs by impairing HR. A) Cytotoxicity of PANP on 4 T1, CT26, B16F10, LCC and NIH/3 T3 cells. Data are portrayed as mean ± standard deviation (n = 3). Statistical significance was assessed through ANOVA and deemed significant at P < 0.05. B) Proposed mechanisms of BRD4 inhibition exacerbates DSBs by impairing HR. Dashed lines guide tumor cell death, while solid lines may lead to tumor survival. C-E) Immunofluorescence and quantitative results of C) γH2AX, D) BRCA1 and E) RAD51 on CT26 cells exposed to the formulations (200 nM) for a 24-h duration. Not treated cells were used as blank. Scale bar = 10 μm. Data are portrayed as mean ± standard deviation (n = 3). Statistical significance was assessed through ANOVA and deemed significant at P < 0.05.

Article Snippet: Antibodies: Phospho-Histone H2AX-S139 Rabbit mAb (Abclonal, AP0687), BRCA1 rabbit pAb (Abclonal, A11034), RAD51 rabbit pAb (Abclonal, A6268), Ki67 Rabbit pAb (Abclonal, A11390), Cy3 Goat AntiRabbit IgG (Abclonal, A5007) and FITC Goat Anti-Rabbit IgG (Abclonal, AS011).

Techniques: Inhibition, Standard Deviation, Immunofluorescence

Journal: Journal of controlled release : official journal of the Controlled Release Society

Article Title: Dual-targeted nanoparticulate drug delivery systems for enhancing triple-negative breast cancer treatment.

doi: 10.1016/j.jconrel.2024.06.012

Figure Lengend Snippet: Fig. 7. SJNP grantees biosafety of treatment without additional hepatorenal toxicity, and elicits binary tumor-killing mechanisms on murine TNBC model. A) Hepatorenal function parameters of of the mice at the end of treatment. Data are portrayed as mean ± standard deviation (n = 3). Statistical significance was assessed through ANOVA and deemed unsignificant at P > 0.05. B) Quantitative results of TUNEL assay and immunofluorescence staining. Data are portrayed as mean ± standard deviation (n = 3). Statistical significance was assessed through ANOVA and deemed significant at P < 0.05. (C, D, E, F and G) Immunofluorescence staining of C) γH2AX, D) BRCA1, E) RAD51, F) TUNEL assay and G) Ki67. Scale bar = 50 μm. H) H&E staining. Scale bar = 50 μm.

Article Snippet: Antibodies: Phospho-Histone H2AX-S139 Rabbit mAb (Abclonal, AP0687), BRCA1 rabbit pAb (Abclonal, A11034), RAD51 rabbit pAb (Abclonal, A6268), Ki67 Rabbit pAb (Abclonal, A11390), Cy3 Goat AntiRabbit IgG (Abclonal, A5007) and FITC Goat Anti-Rabbit IgG (Abclonal, AS011).

Techniques: Standard Deviation, TUNEL Assay, Immunofluorescence, Staining

Journal: Toxicological Sciences

Article Title: Aneugen Molecular Mechanism Assay: Proof-of-Concept With 27 Reference Chemicals

doi: 10.1093/toxsci/kfz123

Figure Lengend Snippet: Unsupervised clustering results are shown as a 2-dimensional dendrogram for 26 chemicals. These results are based on 488 Taxol and p-H3: Ki-67 ratios measured in the MultiFlow Aneugen Molecular Mechanism Assay. Four clades are evident that subgroup aneugens according to molecular target: AK(s), Aurora kinase B inhibitors; TB-S, tubulin stabilizers; TB-D, tubulin destabilizers; Other, aneugenicity via other/indeterminate mechanisms. The bottom-most graph shows the horizontal distances between join points.

Article Snippet: Upon addition of test chemical, the plates were immediately incubated in a humidified atmosphere at 37°C with 5% CO 2 for 24 h. MultiFlow DNA Damage Assay: sample processing TK6 cells were prepared for flow cytometric analysis using reagents and instructions included in the MultiFlow DNA Damage Kit—p53, γH2AX, Phospho-Histone H3 (Litron Laboratories, Rochester, New York).

Techniques:

Journal: Cell Transplantation

Article Title: Proliferation and Differentiation Potential of Bone Marrow–Derived Mesenchymal Stem Cells From Children With Polydactyly and Adults With Basal Joint Arthritis

doi: 10.1177/09636897231221878

Figure Lengend Snippet: Senescence-related genes expression in adult and infant BMSC. The expression levels of (A) p16 , (B) p21 , (C) p53 , and (D) TERT genes in adult and infant BMSCs at early passages (P3–P5) and late passages (P7–P9) were analyzed using RT-qPCR. The expression values were normalized to the reference gene GAPDH . The data are presented as mean ± SEM from three experimental replicates. Using the data from adult BMSCs in the early stage as the baseline, we calculated the relative fold change of adult BMSCs in the late stage and infant BMSCs in both early and late stages. The statistical comparisons of differences were individually analyzed using Mann–Whitney U analysis for the following groups: differences between adult and infant BMSCs in the early passage, differences between adult and infant BMSCs in the late passages, differences between adult BMSCs in the early and late stages, and statistical comparisons between infant BMSCs in the early and late stages. (E) SA-β-gal staining was performed using a Senescence Detection Kit (BioVision, USA) to detect cell senescence in adult and infant BMSCs at passage 8. SA-β-gal positive BMSCs exhibited a blue color upon staining. The cells were visualized using an Olympus BX43 microscope at 200× magnification, and the scale bar represents 150 μm. The percentage of SA-β-gal positive cells was quantified. (F) Immunofluorescence staining was performed on cells at passage 7 using an anti-γH2AX antibody (green) and DAPI (blue). The stained cells were visualized using an Olympus BX43 microscope at 400× magnification, and the scale bar represents 50 μm. The immunofluorescence intensity of γH2AX in the nuclei of adult and infant BMSCs was quantified using Image-Pro Plus v4.5.0.29, normalized to the cell numbers, and presented as fluorescence intensity (arbitrary unit, a.u.). The mean ± SEM calculated from three experimental replicates within both the infant and adult groups. BMSC: bone marrow–derived mesenchymal stem cell; GAPDH : glyceraldehyde-3-phosphate dehydrogenase; SEM: standard error of the mean; SA-β-gal: Senescence-associated β-galactosidase; DAPI: 4′,6-diamidino-2-phenylindole; RT-qPCR: real-time quantitative polymerase chain reaction; TERT: telomerase reverse transcriptase. Statistical significance is indicated by * P < 0.05; *** P < 0.001.

Article Snippet: The adult and infant BMSCs at passage three to five were permeabilized by a permeabilization buffer (0.1% Triton X-100 in PBS) and treated with primary antibodies against γH2AX (tcba13051; Taiclone Biotech Corp., Taipei, Taiwan).

Techniques: Expressing, Quantitative RT-PCR, MANN-WHITNEY, Staining, Microscopy, Immunofluorescence, Fluorescence, Derivative Assay, Real-time Polymerase Chain Reaction, Reverse Transcription