Journal: Biology

Article Title: Medical Biology of Cancer-Associated Fibroblasts in Pancreatic Cancer

doi: 10.3390/biology12081044

Figure Lengend Snippet: Main markers and functions of relevant CAF subtypes in pancreatic cancer. This table summarizes the main markers and functions of three different CAF subtypes that are relevant in pancreatic cancer: myofibrotic CAFs (myCAFs), inflammatory CAFs (iCAFs), and antigen-presenting CAFs (apCAFs).

Article Snippet: Bachem et al. was able to isolate and compare αSMA+ PSCs from both human PDAC and chronic pancreatitis samples, and revealed that they were morphologically similar, expressing collagen, fibronectin, and desmin [ , ].

Techniques: Immunopeptidomics

Journal: Biology

Article Title: Medical Biology of Cancer-Associated Fibroblasts in Pancreatic Cancer

doi: 10.3390/biology12081044

Figure Lengend Snippet: Cellular Origins of CAFs in Pancreatic Cancer. This figure depicts the different cell types that can be activated by various growth factors, cytokines, and/or physiological conditions to transition into CAFs. These cell types include resident fibroblasts, pancreatic stellate cells, mesenchymal stem cells, epithelial cells, endothelial cells, adipocytes, pericytes, fibrocytes, and smooth muscle cells. These diverse cell sources contribute to the pool of CAFs in the TME. Some key markers associated with CAFs are alpha-smooth muscle actin (αSMA) and fibroblast specific protein-1 (FSP-1), also known as S100A4. This figure was reprinted with permission from Ref. . 2019, Liu et al.

Article Snippet: Bachem et al. was able to isolate and compare αSMA+ PSCs from both human PDAC and chronic pancreatitis samples, and revealed that they were morphologically similar, expressing collagen, fibronectin, and desmin [ , ].

Techniques:

Journal: British Journal of Pharmacology

Article Title: Characterization of the properties of a selective, orally bioavailable autotaxin inhibitor in preclinical models of advanced stages of liver fibrosis

doi: 10.1111/bph.14118

Figure Lengend Snippet: Biochemical quantification of hepatic hydroxyproline (HYP) content and αSMA in models of chronic liver injury in rats. (A) Hepatic HYP content in control, CCl4/Vehicle‐ and CCl4/Ex_31‐treated rats. (B) Hepatic αSMA content in control, CCl4/Vehicle‐ and CCl4/Ex_31‐treated rats. (C) Hepatic HYP content in CSAA or CDAA diet‐fed rats treated with Vehicle or Ex_31. (D) Hepatic αSMA content in CSAA or CDAA diet‐fed rats treated with Vehicle or Ex_31. Values are normalized versus liver protein content. Data represent mean ± SEM, n = 10 (control), n = 13 (CCl4‐treated) and n = 12 (CSAA‐ and CDAA‐fed), *P < 0.05.

Article Snippet: An MSD Western‐replacement method (Meso Scale Discovery) using specific anti‐α smooth muscle actin (αSMA) antibody was used to quantify SMA in protein lysates.

Techniques: Control

Journal: Scientific Reports

Article Title: Dose-dependent pro- or anti-fibrotic responses of endometriotic stromal cells to interleukin-1β and tumor necrosis factor α

doi: 10.1038/s41598-020-66298-x

Figure Lengend Snippet: Representative photomicrographs of double immunofluorescence staining for Col I and αSMA in M-ES-healthy, M-ES-endo, and Ectopic-ES after stimulation with IL-1β or TNFα alone or following TGF-β1 5 ng/mL stimulation. Scale bar: 50 μm. Effects of the highest dose of IL-1β (100 pg/mL) on protein expression of Col I and αSMA in M-ES-healthy and M-ES-endo were excluded for further analyses, due to markedly reduced cell viability.

Article Snippet: Endometriotic or endometrial stromal cells were seeded onto glass coverslips (22 mm × 22 mm) (3 × 10 4 cells per coverslip), and were incubated with 2 mL culture media (2% charcoal-stripped FBS) containing IL-1β and/or TNFα at the indicated concentrations or vehicle only for 48 h. In another set, cells were pre-treated with IL-1β, and/or TNFα at the indicated concentrations for 48 h, followed by TGF-β1 (5 ng/mL) stimulation for 48 h. Double immunofluorescence staining for Col I (rabbit polyclonal, 1:500; Abcam, Cambridge, UK) and αSMA (1A4, 1:100; Merck Millipore) was performed in endometrial and endometriotic stromal cells, as previously reported , .

Techniques: Double Immunofluorescence Staining, Expressing