αpd-1-abd-pe Search Results


αpd 1 abd pe  (Thermo Fisher)
86
Thermo Fisher αpd 1 abd pe
(a) The sequential configurations of the functional domains, <t>αPD-1,</t> ABD, and PE, in αPD-1, ABD-PE, αPD-1-PE, and αPD-1-ABD-PE. The linker, (GGGGS) 3 , is shown as an orange box. (b) The amino acid sequences of αPD-1 V H and V L with their framework regions (FRs) and complementarity-determining regions (CDRs) highlighted with red text. Two mutations were introduced in the V H and V L , respectively. The two mutations are underlined, V H : R45C; V L : G104C. (c) The mean fluorescence intensity (MFI) of PD-1 + and PD-1 − primary T cells after the cells were incubated with Alexa Fluor 647-labeled αPD-1-ABD-PE or Alexa Fluor 647-labeled ABD-PE at 4°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their standard deviations (SDs) are indicated (N=6 biologically independent samples; unpaired two-sided t-test). The MFI was obtained by flow cytometry. (d) The MFI of PD-1 + and PD-1 − primary B cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 4°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their SDs are indicated (N=6 biologically independent samples, unpaired two-sided t-test). (e) The MFI of PD-1 + and PD-1 − primary T cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 37°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). (f) The MFI of PD-1 + and PD-1 − primary B cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 37°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFIs and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). (g) The MFI of EL4 cells after the cells were incubated with the labeled αPD-1-ABD-PE under the conditions noted in the figure. The MFIs and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). Studies described in the panels c-g were repeated twice with similar results. Data of one repeat is shown here.
αpd 1 Abd Pe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apd-1-abd-pe  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology apd-1-abd-pe
(a) The sequential configurations of the functional domains, <t>αPD-1,</t> ABD, and PE, in αPD-1, ABD-PE, αPD-1-PE, and αPD-1-ABD-PE. The linker, (GGGGS) 3 , is shown as an orange box. (b) The amino acid sequences of αPD-1 V H and V L with their framework regions (FRs) and complementarity-determining regions (CDRs) highlighted with red text. Two mutations were introduced in the V H and V L , respectively. The two mutations are underlined, V H : R45C; V L : G104C. (c) The mean fluorescence intensity (MFI) of PD-1 + and PD-1 − primary T cells after the cells were incubated with Alexa Fluor 647-labeled αPD-1-ABD-PE or Alexa Fluor 647-labeled ABD-PE at 4°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their standard deviations (SDs) are indicated (N=6 biologically independent samples; unpaired two-sided t-test). The MFI was obtained by flow cytometry. (d) The MFI of PD-1 + and PD-1 − primary B cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 4°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their SDs are indicated (N=6 biologically independent samples, unpaired two-sided t-test). (e) The MFI of PD-1 + and PD-1 − primary T cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 37°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). (f) The MFI of PD-1 + and PD-1 − primary B cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 37°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFIs and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). (g) The MFI of EL4 cells after the cells were incubated with the labeled αPD-1-ABD-PE under the conditions noted in the figure. The MFIs and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). Studies described in the panels c-g were repeated twice with similar results. Data of one repeat is shown here.
Apd 1 Abd Pe, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apd-1-abd-pe/product/Santa Cruz Biotechnology
Average 90 stars, based on 1 article reviews
apd-1-abd-pe - by Bioz Stars, 2026-02
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Image Search Results


(a) The sequential configurations of the functional domains, αPD-1, ABD, and PE, in αPD-1, ABD-PE, αPD-1-PE, and αPD-1-ABD-PE. The linker, (GGGGS) 3 , is shown as an orange box. (b) The amino acid sequences of αPD-1 V H and V L with their framework regions (FRs) and complementarity-determining regions (CDRs) highlighted with red text. Two mutations were introduced in the V H and V L , respectively. The two mutations are underlined, V H : R45C; V L : G104C. (c) The mean fluorescence intensity (MFI) of PD-1 + and PD-1 − primary T cells after the cells were incubated with Alexa Fluor 647-labeled αPD-1-ABD-PE or Alexa Fluor 647-labeled ABD-PE at 4°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their standard deviations (SDs) are indicated (N=6 biologically independent samples; unpaired two-sided t-test). The MFI was obtained by flow cytometry. (d) The MFI of PD-1 + and PD-1 − primary B cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 4°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their SDs are indicated (N=6 biologically independent samples, unpaired two-sided t-test). (e) The MFI of PD-1 + and PD-1 − primary T cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 37°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). (f) The MFI of PD-1 + and PD-1 − primary B cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 37°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFIs and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). (g) The MFI of EL4 cells after the cells were incubated with the labeled αPD-1-ABD-PE under the conditions noted in the figure. The MFIs and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). Studies described in the panels c-g were repeated twice with similar results. Data of one repeat is shown here.

Journal: Nature biomedical engineering

Article Title: Depletion of PD-1-positive cells ameliorates autoimmune disease

doi: 10.1038/s41551-019-0360-0

Figure Lengend Snippet: (a) The sequential configurations of the functional domains, αPD-1, ABD, and PE, in αPD-1, ABD-PE, αPD-1-PE, and αPD-1-ABD-PE. The linker, (GGGGS) 3 , is shown as an orange box. (b) The amino acid sequences of αPD-1 V H and V L with their framework regions (FRs) and complementarity-determining regions (CDRs) highlighted with red text. Two mutations were introduced in the V H and V L , respectively. The two mutations are underlined, V H : R45C; V L : G104C. (c) The mean fluorescence intensity (MFI) of PD-1 + and PD-1 − primary T cells after the cells were incubated with Alexa Fluor 647-labeled αPD-1-ABD-PE or Alexa Fluor 647-labeled ABD-PE at 4°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their standard deviations (SDs) are indicated (N=6 biologically independent samples; unpaired two-sided t-test). The MFI was obtained by flow cytometry. (d) The MFI of PD-1 + and PD-1 − primary B cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 4°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their SDs are indicated (N=6 biologically independent samples, unpaired two-sided t-test). (e) The MFI of PD-1 + and PD-1 − primary T cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 37°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFI means and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). (f) The MFI of PD-1 + and PD-1 − primary B cells after the cells were incubated with the labeled αPD-1-ABD-PE or the labeled ABD-PE at 37°C for 30 minutes. The cells were collected from C57BL/6 mice. The MFIs and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). (g) The MFI of EL4 cells after the cells were incubated with the labeled αPD-1-ABD-PE under the conditions noted in the figure. The MFIs and their SDs are indicated (N=6 biologically independent samples; unpaired two-sided t-test). Studies described in the panels c-g were repeated twice with similar results. Data of one repeat is shown here.

Article Snippet: Five nmole of αPD-1-ABD-PE or αPD-1-PE that were labeled by NHS-Fluorescein (Thermo Fisher Scientific Inc) were injected intraperitoneally into C57BL/6 mice.

Techniques: Functional Assay, Fluorescence, Incubation, Labeling, Flow Cytometry

(a) The relative viability of PD-1 + and PD-1 − primary T cells after they were incubated with αPD-1-ABD-PE or a control mixture of αPD-1 and ABD-PE for 72 hours. The mean viabilities and their SDs at different concentrations of αPD-1-ABD-PE and the control mixture were shown. The viability data of PD-1 + primary T cells after the αPD-1-ABD-PE treatment were fitted to a Sigmoidal dose-response model and the IC 50 was obtained through the fitting (N=6 biologically independent samples). The cells were collected from C57BL/6 mice. (b) The relative viability of PD-1 + and PD-1 − primary B cells after they were incubated with αPD-1-ABD-PE or a control mixture of αPD-1 and ABD-PE for 72 hours. The mean viabilities and their SDs at different concentrations of αPD-1-ABD-PE and the control mixture were shown (N=6 biologically independent samples). The cells were collected from C57BL/6 mice. (c) The relative viability of wildtype EL4 and PD-1 − EL4 cells after they were incubated with αPD-1-ABD-PE or a control mixture of αPD-1 and ABD-PE for 72 hours. The mean viabilities and their SDs at different concentrations of αPD-1-ABD-PE and the control mixture were shown and fitted to a Sigmoidal dose-response model (N=6 biologically independent samples). (d) The fractions of transferred EL4 cells among lymphocytes. These lymphocytes were collected from mice at 72 hours after these mice were treated with αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS. The mean faction values and their SDs are indicated (N=3 mice). All studies described in this figure were repeated twice with similar results. Data of one repeat is shown here.

Journal: Nature biomedical engineering

Article Title: Depletion of PD-1-positive cells ameliorates autoimmune disease

doi: 10.1038/s41551-019-0360-0

Figure Lengend Snippet: (a) The relative viability of PD-1 + and PD-1 − primary T cells after they were incubated with αPD-1-ABD-PE or a control mixture of αPD-1 and ABD-PE for 72 hours. The mean viabilities and their SDs at different concentrations of αPD-1-ABD-PE and the control mixture were shown. The viability data of PD-1 + primary T cells after the αPD-1-ABD-PE treatment were fitted to a Sigmoidal dose-response model and the IC 50 was obtained through the fitting (N=6 biologically independent samples). The cells were collected from C57BL/6 mice. (b) The relative viability of PD-1 + and PD-1 − primary B cells after they were incubated with αPD-1-ABD-PE or a control mixture of αPD-1 and ABD-PE for 72 hours. The mean viabilities and their SDs at different concentrations of αPD-1-ABD-PE and the control mixture were shown (N=6 biologically independent samples). The cells were collected from C57BL/6 mice. (c) The relative viability of wildtype EL4 and PD-1 − EL4 cells after they were incubated with αPD-1-ABD-PE or a control mixture of αPD-1 and ABD-PE for 72 hours. The mean viabilities and their SDs at different concentrations of αPD-1-ABD-PE and the control mixture were shown and fitted to a Sigmoidal dose-response model (N=6 biologically independent samples). (d) The fractions of transferred EL4 cells among lymphocytes. These lymphocytes were collected from mice at 72 hours after these mice were treated with αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS. The mean faction values and their SDs are indicated (N=3 mice). All studies described in this figure were repeated twice with similar results. Data of one repeat is shown here.

Article Snippet: Five nmole of αPD-1-ABD-PE or αPD-1-PE that were labeled by NHS-Fluorescein (Thermo Fisher Scientific Inc) were injected intraperitoneally into C57BL/6 mice.

Techniques: Incubation

(a) A photo of native PAGE gel that demonstrates the association between αPD-1-ABD-PE and MSA. αPD-1-ABD-PE and MSA were mixed the 1-to-1 ratio. αPD-1-PE and MSA were also mixed the 1-to-1 ratio. (b) A photo of native PAGE gel that demonstrates the association between αPD-1-ABD-PE and HSA. αPD-1-ABD-PE and HSA were mixed at the 1-to-1 ratio. αPD-1-PE and HSA were also mixed at the 1-to-1 ratio. Studies described in the panels a-b were repeated independently at least three times showing similar results. The representative data were shown here. (c) The plasma concentration versus time profiles of αPD-1-ABD-PE and αPD-1-PE after the two protein were intraperitoneally injected into mice at the same dose 5 nmol per mouse (N=3 biologically independent samples: error bars represent SD with the measure of center as mean values). The PK data was analyzed using non-compartmental model. Each dot represents a plasma concentration value at a given time points.

Journal: Nature biomedical engineering

Article Title: Depletion of PD-1-positive cells ameliorates autoimmune disease

doi: 10.1038/s41551-019-0360-0

Figure Lengend Snippet: (a) A photo of native PAGE gel that demonstrates the association between αPD-1-ABD-PE and MSA. αPD-1-ABD-PE and MSA were mixed the 1-to-1 ratio. αPD-1-PE and MSA were also mixed the 1-to-1 ratio. (b) A photo of native PAGE gel that demonstrates the association between αPD-1-ABD-PE and HSA. αPD-1-ABD-PE and HSA were mixed at the 1-to-1 ratio. αPD-1-PE and HSA were also mixed at the 1-to-1 ratio. Studies described in the panels a-b were repeated independently at least three times showing similar results. The representative data were shown here. (c) The plasma concentration versus time profiles of αPD-1-ABD-PE and αPD-1-PE after the two protein were intraperitoneally injected into mice at the same dose 5 nmol per mouse (N=3 biologically independent samples: error bars represent SD with the measure of center as mean values). The PK data was analyzed using non-compartmental model. Each dot represents a plasma concentration value at a given time points.

Article Snippet: Five nmole of αPD-1-ABD-PE or αPD-1-PE that were labeled by NHS-Fluorescein (Thermo Fisher Scientific Inc) were injected intraperitoneally into C57BL/6 mice.

Techniques: Clear Native PAGE, Concentration Assay, Injection

(a) The diabetes-free survival of NOD mice that were treated with αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS weekly since there mice were 12 weeks old (N=5 mice). (b) The diabetes-free survival of NOD mice that were treated five times with αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS (N=5 mice). The arrows indicate the five dosing dates of the treatments. Before these treatments, all of these mice were treated with CP at day 0. (c) The fraction of PD-1 + cells among collected pancreatic cells from 18-week old NOD mice after these mice were treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS. Each dot represents the fraction value of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (d) The fraction of PD-1 + CD4 T cells among the pancreatic cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (e) The fraction of PD-1 + CD8 T cells among the pancreatic cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (f) The fraction of CD4 T cells among the pancreatic cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (g) The fraction of CD8 T cells among the pancreatic cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (h) The fraction of B cells among the pancreatic cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (i) The diabetes-free survival of NOD mice that were first treated with αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS, and then with αPD-1 (full IgG). (N=5 mice; unpaired two-sided t-test) The survival of αPD-1-ABD-PE treated mice is significantly different to PBS and the control mixture treated mice (P=0.0494 and P=0.0018, respectively). All studies described in this figure were repeated twice with similar results. Data of one repeat is shown here.

Journal: Nature biomedical engineering

Article Title: Depletion of PD-1-positive cells ameliorates autoimmune disease

doi: 10.1038/s41551-019-0360-0

Figure Lengend Snippet: (a) The diabetes-free survival of NOD mice that were treated with αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS weekly since there mice were 12 weeks old (N=5 mice). (b) The diabetes-free survival of NOD mice that were treated five times with αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS (N=5 mice). The arrows indicate the five dosing dates of the treatments. Before these treatments, all of these mice were treated with CP at day 0. (c) The fraction of PD-1 + cells among collected pancreatic cells from 18-week old NOD mice after these mice were treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS. Each dot represents the fraction value of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (d) The fraction of PD-1 + CD4 T cells among the pancreatic cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (e) The fraction of PD-1 + CD8 T cells among the pancreatic cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (f) The fraction of CD4 T cells among the pancreatic cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (g) The fraction of CD8 T cells among the pancreatic cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (h) The fraction of B cells among the pancreatic cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (i) The diabetes-free survival of NOD mice that were first treated with αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS, and then with αPD-1 (full IgG). (N=5 mice; unpaired two-sided t-test) The survival of αPD-1-ABD-PE treated mice is significantly different to PBS and the control mixture treated mice (P=0.0494 and P=0.0018, respectively). All studies described in this figure were repeated twice with similar results. Data of one repeat is shown here.

Article Snippet: Five nmole of αPD-1-ABD-PE or αPD-1-PE that were labeled by NHS-Fluorescein (Thermo Fisher Scientific Inc) were injected intraperitoneally into C57BL/6 mice.

Techniques:

(a) The clinical score changes of the mice with EAE that were treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS. The shown data are mean scores and their standard deviations at each observation time point after the induction of EAE. (N=6 mice). The X-axis indicates the number of days after EAE induction. (b) The clinical score changes of the mice described in (a). The shown data are mean scores and their standard deviations at each observation time point after the treatments. The mean clinical score of the αPD-1-ABD-PE group was different from the score of the PBS group since day 5 post treatment (*P<0.0002; unpaired two-sided t-test). “^” was used to denote those days when the mean clinical scores of the two groups were apparently different however t-test could not be conducted because the scores of the both groups have no variation. The X-axis indicates the number of days since an individual mouse received treatments. The experiment described in “ a ” and “ b ” was repeated trice and the data of one repeat is shown here. (c) The fraction of PD-1 + cells among the collected mononuclear cells from the CNS of the mice that were treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS. Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (d) The fraction of PD-1 + CD4 T cells among the mononuclear cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (e) The fraction of PD-1 + CD8 T cells among the mononuclear cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (f) The fraction of CD4 T cells among the mononuclear cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (g) The fraction of CD8 T cells among the mononuclear cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (h) The fraction of B cells among the mononuclear cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (i) The ratios between Tregs and the MOG-specific CD4 T cells in the mononuclear cells described in (c). Each dot represents the ratio result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). All studies described in this figure were repeated twice with similar results. Data of one repeat is shown here.

Journal: Nature biomedical engineering

Article Title: Depletion of PD-1-positive cells ameliorates autoimmune disease

doi: 10.1038/s41551-019-0360-0

Figure Lengend Snippet: (a) The clinical score changes of the mice with EAE that were treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS. The shown data are mean scores and their standard deviations at each observation time point after the induction of EAE. (N=6 mice). The X-axis indicates the number of days after EAE induction. (b) The clinical score changes of the mice described in (a). The shown data are mean scores and their standard deviations at each observation time point after the treatments. The mean clinical score of the αPD-1-ABD-PE group was different from the score of the PBS group since day 5 post treatment (*P<0.0002; unpaired two-sided t-test). “^” was used to denote those days when the mean clinical scores of the two groups were apparently different however t-test could not be conducted because the scores of the both groups have no variation. The X-axis indicates the number of days since an individual mouse received treatments. The experiment described in “ a ” and “ b ” was repeated trice and the data of one repeat is shown here. (c) The fraction of PD-1 + cells among the collected mononuclear cells from the CNS of the mice that were treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, or PBS. Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated. (N=6 mice; unpaired two-sided t-test). (d) The fraction of PD-1 + CD4 T cells among the mononuclear cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (e) The fraction of PD-1 + CD8 T cells among the mononuclear cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (f) The fraction of CD4 T cells among the mononuclear cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (g) The fraction of CD8 T cells among the mononuclear cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (h) The fraction of B cells among the mononuclear cells described in (c). Each dot represents the fraction result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (i) The ratios between Tregs and the MOG-specific CD4 T cells in the mononuclear cells described in (c). Each dot represents the ratio result of a single mouse. The fraction means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). All studies described in this figure were repeated twice with similar results. Data of one repeat is shown here.

Article Snippet: Five nmole of αPD-1-ABD-PE or αPD-1-PE that were labeled by NHS-Fluorescein (Thermo Fisher Scientific Inc) were injected intraperitoneally into C57BL/6 mice.

Techniques:

(a) B220+, CD4+, CD8+ cell numbers in blood and spleens in the C57BL/6 mice that were treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, PBS, or CP. The cell number means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (b) ELISA results of the anti-DNP humoral responses in the C57BL/6 and NOD mice that that were pre-treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, PBS, or CP. The results were measured by OD 450 after a background OD 570 subtraction. The mean ± SD of OD 450 for the serum samples at indicated dilutions were shown. The same samples were loaded into both DNP-BSA-coated and BSA-coated (control) ELISA plates, separately. The materials used to coat the plates are written in the parentheses. (N=6 mice). (c) ELISPOT results of the CTL responses in the C57BL/6 and NOD mice that were treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, PBS, or CP. The Y-axis represents the number of IFN-γ-positive spots resulting from the 500,000 splenocytes collected from these treated mice. The means and their SDs of the spot numbers are indicated. (N=6 mice; unpaired two-sided t-test; for the data of C57BL/6 mice, ns, not significant, P=0.9314 unpaired t-test; for the data of NOD mice, ns, not significant, P=0.9429). All studies described in this figure were repeated at least twice with similar results. Data of one repeat is shown here.

Journal: Nature biomedical engineering

Article Title: Depletion of PD-1-positive cells ameliorates autoimmune disease

doi: 10.1038/s41551-019-0360-0

Figure Lengend Snippet: (a) B220+, CD4+, CD8+ cell numbers in blood and spleens in the C57BL/6 mice that were treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, PBS, or CP. The cell number means and their SDs are indicated (N=6 mice; unpaired two-sided t-test). (b) ELISA results of the anti-DNP humoral responses in the C57BL/6 and NOD mice that that were pre-treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, PBS, or CP. The results were measured by OD 450 after a background OD 570 subtraction. The mean ± SD of OD 450 for the serum samples at indicated dilutions were shown. The same samples were loaded into both DNP-BSA-coated and BSA-coated (control) ELISA plates, separately. The materials used to coat the plates are written in the parentheses. (N=6 mice). (c) ELISPOT results of the CTL responses in the C57BL/6 and NOD mice that were treated with one dose of αPD-1-ABD-PE, a control mixture of αPD-1 and ABD-PE, PBS, or CP. The Y-axis represents the number of IFN-γ-positive spots resulting from the 500,000 splenocytes collected from these treated mice. The means and their SDs of the spot numbers are indicated. (N=6 mice; unpaired two-sided t-test; for the data of C57BL/6 mice, ns, not significant, P=0.9314 unpaired t-test; for the data of NOD mice, ns, not significant, P=0.9429). All studies described in this figure were repeated at least twice with similar results. Data of one repeat is shown here.

Article Snippet: Five nmole of αPD-1-ABD-PE or αPD-1-PE that were labeled by NHS-Fluorescein (Thermo Fisher Scientific Inc) were injected intraperitoneally into C57BL/6 mice.

Techniques: Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot