Journal: BMJ Neurology Open

Article Title: Altered amyloid plasma profile in patients with disabling headaches after SARS-CoV-2 infection and vaccination

doi: 10.1136/bmjno-2024-001013

Figure Lengend Snippet: Plasma levels of (A) amyloid precursor protein (APP), (B) cathepsin L, (C) pregnancy zone protein (PZP) and (D) serum amyloid A (SAA1) in healthy controls (HC) (n=16), participants with persistent headache after SARS-CoV-2 vaccine (COvax) (n=31) and participants with persistent headache after COVID-19 ( C19 ) (n=29).

Article Snippet: Plasma levels of APP (Cat# DY850), PZP (Cat# DY8280-05), CTSL (Cat# DY952) and SAA1 (Cat# DY3019-05) were measured by ELISA using commercially available antibodies (R&D Systems, Minneapolis, Minnesota, USA) in a 384-format using a combination of a SELMA pipetting robot (Analytik Jena AG, Jena, Germany) and a BioTek dispenser/washer (BioTek Instruments, Winooski, VT).

Techniques: Clinical Proteomics

Journal: Frontiers in Immunology

Article Title: TMT-Based Quantitative Proteomics Analysis of Synovial Fluid-Derived Exosomes in Inflammatory Arthritis

doi: 10.3389/fimmu.2022.800902

Figure Lengend Snippet: Verification of exosome-derived PZP in SF by ELISA. The ELISA kits were performed to detect the PZP level of SF-derived exosomes in gout, axSpA, OA, and RA. *P value < 0.05 vs. OA groups.

Article Snippet: The ELISA Kits (CSB-EL019131HU, CUSABIO BIOTECH) were performed to detect the pregnancy zone protein (PZP) level of SF-derived exosomes in all groups, following the manufacturer’s instructions.

Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay

Journal: Clinical Proteomics

Article Title: iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy

doi: 10.1186/s12014-019-9253-1

Figure Lengend Snippet: MS/MS spectrum for iTRAQ quantification. A representative MS/MS spectrum indicated important peptide segments for gelsolin, collectin-11, PTPRJ, AKAP-7, pregnancy zone protein and transthyretin. iTRAQ tags showed the relative expression of the six proteins individually in the plasma of type 2 incipient diabetic nephropathy patients compared to the control

Article Snippet: Primary antibodies against gelsolin (Abcam, #109014), transthyretin (Abcam, #92469), pregnancy zone protein (Proteintech, #21742), A-kinase anchor protein 7 (Proteintech, #12591), PTPRJ (Proteintech, #55123), and collectin-11 (Proteintech, #15269) were incubated overnight, followed by the appropriate horseradish peroxidase-conjugated secondary antibodies.

Techniques: Tandem Mass Spectroscopy, Multiplex sample analysis, Expressing, Clinical Proteomics, Control

Journal: Clinical Proteomics

Article Title: iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy

doi: 10.1186/s12014-019-9253-1

Figure Lengend Snippet: Western blot confirmation of six selected proteins. Representative WB images for six proteins, including gelsolin, collectin-11, PTPRJ and AKAP-7, pregnancy zone protein and transthyretin. Data are expressed as the mean using GraphPad Prism. ****p < 0.0001. Controls (n = 62), IDN patients (n = 62)

Article Snippet: Primary antibodies against gelsolin (Abcam, #109014), transthyretin (Abcam, #92469), pregnancy zone protein (Proteintech, #21742), A-kinase anchor protein 7 (Proteintech, #12591), PTPRJ (Proteintech, #55123), and collectin-11 (Proteintech, #15269) were incubated overnight, followed by the appropriate horseradish peroxidase-conjugated secondary antibodies.

Techniques: Western Blot