Journal: Cell Death & Disease

Article Title: Consequences of Zmat3 loss in c-MYC - and mutant KRAS -driven tumorigenesis

doi: 10.1038/s41419-020-03066-9

Figure Lengend Snippet: a Zmat3 mRNA expression in pre-leukemic Eµ-Myc B lymphoid cells 0, 6, 12 and 24 h following 5 Gy γ-irradiation (IR) or treatment with 10 µM Nutlin3a (Nut3a). Mean ± SEM. b Lymphoma-free survival of Eµ-Myc ( n = 23), Eµ-Myc ; Zmat3 +/ − ( n = 21) and Eµ-Myc ; Zmat3 −/ − ( n = 17) mice. Median lymphoma-free survival: Eµ-Myc : 125 days; Eµ-Myc ; Zmat3 +/− : 94 days; Eµ-Myc ; Zmat3 −/ − : 93 days. Eµ-Myc vs Eµ-Myc ; Zmat3 +/ − p = 0.0718; Eµ-Myc vs Eµ-Myc ; Zmat3 −/ − p = 0.3231. c The proportions of Ig + B cell lymphomas and pro-B/pre-B cell lymphomas in sick Eµ - Myc ; Zmat3 −/− ( n = 4) and sick Eµ - Myc ( n = 3) mice. No significant differences were observed; p = 0.5 calculated by two-tailed t -test. d Spleen, thymus and lymph node weight (grams) in sick Eµ-Myc ( n = 7), Eµ-Myc ; Zmat3 +/ − ( n = 15) and Eµ-Myc ; Zmat3 −/ − ( n = 9) mice at ethical endpoint. Mean ± SEM.

Article Snippet: Quantitative RT-PCR was performed using TaqMan probes (Thermo Fisher Scientific) for Zmat3 (Mm01292424_m1), Puma (Mm00519268_m1), p21 (Mm00432448_m1) and analyzed by the ΔΔCT method relative to HMBS (Mm01143545_m1).

Techniques: Expressing, Irradiation, Two Tailed Test

Journal: Cell Death & Disease

Article Title: Consequences of Zmat3 loss in c-MYC - and mutant KRAS -driven tumorigenesis

doi: 10.1038/s41419-020-03066-9

Figure Lengend Snippet: a Representative hematoxylin and eosin (H&E) staining of lungs from Zmat3 −/ − , littermate Zmat3 +/+ (wt) and p53 −/ − control mice aged 16-18 weeks. Scale, 200 μm. b Flow cytometric analysis of the lymphoid and myeloid cells present in the lungs from Zmat3 −/− ( n = 4), p53 −/ − ( n = 2) and wt ( n = 4) mice. Immune cell populations are normalized to wt control mice. c Growth analysis of primary cell lines derived from the lung epithelium of wt ( n = 8) , Zmat3 −/ − ( n = 8) and p53 −/ − ( n = 4) mice. Mean ± SEM. d Accumulative cell number following 8 passages of primary wt ( n = 8) , p53 − / − ( n = 4) and Zmat3 −/− ( n = 8) cell lines. Mean ± SD, **** p < 0.0001. e Representative light microscope images of Zmat3 +/+ (wt) , p53 −/− and Zmat3 −/ − cell lines 8 passages following their derivation. Scale, 10 μm.

Article Snippet: Quantitative RT-PCR was performed using TaqMan probes (Thermo Fisher Scientific) for Zmat3 (Mm01292424_m1), Puma (Mm00519268_m1), p21 (Mm00432448_m1) and analyzed by the ΔΔCT method relative to HMBS (Mm01143545_m1).

Techniques: Staining, Control, Derivative Assay, Light Microscopy

Journal: Cell Death & Disease

Article Title: Consequences of Zmat3 loss in c-MYC - and mutant KRAS -driven tumorigenesis

doi: 10.1038/s41419-020-03066-9

Figure Lengend Snippet: a Schematic of experimental design. Kras LSL-G12D/+ (K) mice were crossed with p53 −/ − mice (KPnull) or Zmat3 −/ − mice (KZ). b Kaplan–Meier tumor-free survival analysis of K ( n = 6), KPnull ( n = 6) and KZ ( n = 12) mice following i.n. infection of Ad5-CMV-Cre virus. c Representative H&E staining and immunohistochemistry of Ki67, Nkx2.1/TTF-1 and Hmga2 in end-stage lung tumors from Kras G12D , Kras G12D ; p53 −/ − and Kras G12D ; Zmat3 −/ − mice. Scale, 100 μm.

Article Snippet: Quantitative RT-PCR was performed using TaqMan probes (Thermo Fisher Scientific) for Zmat3 (Mm01292424_m1), Puma (Mm00519268_m1), p21 (Mm00432448_m1) and analyzed by the ΔΔCT method relative to HMBS (Mm01143545_m1).

Techniques: Infection, Virus, Staining, Immunohistochemistry

Journal: Cell Death & Disease

Article Title: Consequences of Zmat3 loss in c-MYC - and mutant KRAS -driven tumorigenesis

doi: 10.1038/s41419-020-03066-9

Figure Lengend Snippet: a Representative low magnification H&E stained lungs of Kras G12D/+ (K), Kras G12D/+ ; p53 −/− (KPnull) and Kras G12D/+ ; Zmat3 −/− (KZ) mice 6- and 10-weeks following i.n. administration of Ad5-CMV-Cre. Scale, 1 mm. b Lesion classification in the lungs of K ( n = 4), KPnull ( n = 4) and KZ ( n = 5) mice 10 weeks following i.n. Ad5-CMV-Cre infection. c Wet weight (mg) of the left lung lobe of K ( n = 3), KPnull ( n = 3) and KZ ( n = 8) mice 6 weeks following i.n. Ad5-CMV-Cre infection. Violin plot indicates the median weight and distribution.

Article Snippet: Quantitative RT-PCR was performed using TaqMan probes (Thermo Fisher Scientific) for Zmat3 (Mm01292424_m1), Puma (Mm00519268_m1), p21 (Mm00432448_m1) and analyzed by the ΔΔCT method relative to HMBS (Mm01143545_m1).

Techniques: Staining, Infection

Journal: Cell Death & Disease

Article Title: Consequences of Zmat3 loss in c-MYC - and mutant KRAS -driven tumorigenesis

doi: 10.1038/s41419-020-03066-9

Figure Lengend Snippet: a Growth analysis of primary cell lines derived from the lung epithelium of KPnull ( n = 8) and KZ ( n = 12) mice 10 weeks following i.n. Ad5-CMV-Cre infection. Mean ± SEM. b Accumulative cell number following 8 passages of primary KPnull ( n = 8) and KZ ( n = 12) cell lines. Mean ± SD, *** p < 0.0001. c Representative light microscope images of Kras G12D / p53 −/ − (KPnull) and Kras G12D / Zmat3 −/ − (KZ) cell lines 8 passages following their derivation. Scale, 10 μm. d Schematic representation of p53 target genes and their role in tumor suppression.

Article Snippet: Quantitative RT-PCR was performed using TaqMan probes (Thermo Fisher Scientific) for Zmat3 (Mm01292424_m1), Puma (Mm00519268_m1), p21 (Mm00432448_m1) and analyzed by the ΔΔCT method relative to HMBS (Mm01143545_m1).

Techniques: Derivative Assay, Infection, Light Microscopy