Journal: OncoTargets and therapy

Article Title: Decreased expression of SLC 39A14 is associated with tumor aggressiveness and biochemical recurrence of human prostate cancer

doi: 10.2147/OTT.S103640

Figure Lengend Snippet: Decreased expression of SLC39A14 protein and mRNA in human PCa tissues. Notes: ( A ) SLC39A14 protein was mainly localized in the membrane and cytoplasm of prostate cells in adjacent noncancerous prostate tissues. Red arrows show strong positive immunostrainings. Magnification, ×400. ( B ) SLC39A14 protein was weakly expressed in cancer cells in PCa tissues. Magnification, ×400. ( C ) Statistical analysis revealed that SLC39A14 protein expression levels in human PCa tissues were significantly lower than those in adjacent noncancerous prostate tissues ( P <0.01). ( D ) SLC39A14 mRNA expression levels in human PCa tissues were also lower than those in adjacent noncancerous prostate tissues based on the Taylor dataset ( P <0.01). ( E ) SLC39A14 mRNA expression levels in human PCa tissues were also lower than those in adjacent noncancerous prostate tissues based on our clinical samples ( P <0.01). Abbreviations: PCa, prostate cancer; SLC39A14, solute carrier family 39, member 14.

Article Snippet: Rabbit polyclonal ZIP14 antibody (ProSci incorporated, Poway, CA, USA) was used in this study.

Techniques: Expressing, Membrane

Journal: OncoTargets and therapy

Article Title: Decreased expression of SLC 39A14 is associated with tumor aggressiveness and biochemical recurrence of human prostate cancer

doi: 10.2147/OTT.S103640

Figure Lengend Snippet: Kaplan–Meier curves of patients with PCa based on SLC39A14 mRNA expression. Notes: ( A ) There was a significant difference in BCR-free survival between patients with high and low SLC39A14 mRNA expression ( P =0.017). ( B ) There was no significant difference in overall survival between patients with high and low SLC39A14 mRNA expression ( P =0.148). Abbreviations: BCR, biochemical recurrence; PCa, prostate cancer; SLC39A14, solute carrier family 39, member 14.

Article Snippet: Rabbit polyclonal ZIP14 antibody (ProSci incorporated, Poway, CA, USA) was used in this study.

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Decreased expression of SLC 39A14 is associated with tumor aggressiveness and biochemical recurrence of human prostate cancer

doi: 10.2147/OTT.S103640

Figure Lengend Snippet: Downregulation of SLC39A14 promotes cell proliferation of LNCaP cells in vitro. Notes: ( A ) Western blot analysis showed that SLC39A14 protein expression was significantly upregulated by the transfection of SLC39A14 plasmid (en-SLC39A14 or en-con), but was significantly downregulated by the transfection of SLC39A14 siRNA (si-SLC39A14 or si-con). ( B ) CCK-8 assay indicated that the cell viability of LNCaP cells with overexpression of SLC39A14 was significantly lower than those of control vector-transfected cells. ( C ) CCK-8 assay indicated that the cell viability of LNCaP cells with knockdown of SLC39A14 dramatically promoted the cell viability. Abbreviations: CCK-8, Cell Counting Kit-8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SLC39A14, solute carrier family 39, member 14; OD, optical density.

Article Snippet: Rabbit polyclonal ZIP14 antibody (ProSci incorporated, Poway, CA, USA) was used in this study.

Techniques: In Vitro, Western Blot, Expressing, Transfection, Plasmid Preparation, CCK-8 Assay, Over Expression, Control, Knockdown, Cell Counting

Journal: OncoTargets and therapy

Article Title: Decreased expression of SLC 39A14 is associated with tumor aggressiveness and biochemical recurrence of human prostate cancer

doi: 10.2147/OTT.S103640

Figure Lengend Snippet: Downregulation of SLC39A14 promotes invasion of LNCaP cells in vitro. Notes: Transwell assays clearly revealed that downregulation of SLC39A14 significantly enhanced the invasion activity of LNCaP cells compared to that of control cells at 24 hours after the transfection, while overexpression of SLC39A14 dramatically reduced the cell invasion. Cell invasion of LNCaP cells transfected with en-con ( A ), en-SLC39A14 ( B ), si-con ( C ) and si-SLC39A14 ( D ). ( E ) The number of invasive cells after the transfection of en-con and en-SLC39A14. ( F ) The number of invasive cells after the transfection of si-con and si-SLC39A14. Abbreviation: SLC39A14, solute carrier family 39, member 14.

Article Snippet: Rabbit polyclonal ZIP14 antibody (ProSci incorporated, Poway, CA, USA) was used in this study.

Techniques: In Vitro, Activity Assay, Control, Transfection, Over Expression

Journal: OncoTargets and therapy

Article Title: Decreased expression of SLC 39A14 is associated with tumor aggressiveness and biochemical recurrence of human prostate cancer

doi: 10.2147/OTT.S103640

Figure Lengend Snippet: Downregulation of SLC39A14 promotes migration of LNCaP cells in vitro. Notes: ( A ) Cell migration of LNCaP cells transfected with en-con, en-SLC39A14, si-con and si-SLC39A14. ( B ) Inhibition rate of LNCaP cells after the transfection of en-con and en-SLC39A14. ( C ) Inhibition rate of LNCaP cells after the transfection of si-con and si-SLC39A14. Wound-healing assays demonstrated that downregulation of SLC39A14 significantly enhanced the migration activity of LNCaP cells compared to that of control cells at 24 hours after the transfection, while overexpression of SLC39A14 dramatically reduced the cell migration. Abbreviation: SLC39A14, solute carrier family 39, member 14.

Article Snippet: Rabbit polyclonal ZIP14 antibody (ProSci incorporated, Poway, CA, USA) was used in this study.

Techniques: Migration, In Vitro, Transfection, Inhibition, Activity Assay, Control, Over Expression

Journal: Biological Trace Element Research

Article Title: The Role of Zinc on Liver Fibrosis by Modulating ZIP14 Expression Throughout Epigenetic Regulatory Mechanisms

doi: 10.1007/s12011-023-04057-5

Figure Lengend Snippet: Primers used in mouse RT-PCR experiments

Article Snippet: A 50 μL containing 5 × 10 4 cells as the unstained cell group was separated into a separate microcentrifuge tube and the remaining cells were stained with 1 μL of FITC mouse anti-human ZIP14 (MyBioSource, MBS151580) antibody and isotype antibodies respectively to the cells in 100 μL of 5% BSA and 0.05% sodium azide in 1X PBS.

Techniques:

Journal: Biological Trace Element Research

Article Title: The Role of Zinc on Liver Fibrosis by Modulating ZIP14 Expression Throughout Epigenetic Regulatory Mechanisms

doi: 10.1007/s12011-023-04057-5

Figure Lengend Snippet: Effects of ZnCl 2 on the accumulation of MTF-1, histone deacetylase 4 to the promoters of ZIP14 promoter. The Sham group received mineral oil only, CCl 4 and CCl 4 + ZnCl 2 group were injected with 10% CCl 4 in mineral oil for 8 weeks. 10 µM ZnCl 2 was only injected to CCl 4 + ZnCl 2 group for two weeks after CCl 4 treatment was completed. The enrichment of A MTF-1, B HDAC4 on the ZIP14 gene was analyzed by ChIP analysis. Genomic DNA extracted from hepatocytes of A control mice and B mice with CCl 4 -induced hepatic fibrosis were immunoprecipitated with anti-MTF-1 and anti-HDAC4. The change in the accumulation of these proteins on the ZIP14 gene was quantified by qPCR. The data were expressed as a percent of input. An asterisk (*) indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.001. All data are represented as the mean ± SD ( n = 4)

Article Snippet: A 50 μL containing 5 × 10 4 cells as the unstained cell group was separated into a separate microcentrifuge tube and the remaining cells were stained with 1 μL of FITC mouse anti-human ZIP14 (MyBioSource, MBS151580) antibody and isotype antibodies respectively to the cells in 100 μL of 5% BSA and 0.05% sodium azide in 1X PBS.

Techniques: Histone Deacetylase Assay, Injection, Control, Immunoprecipitation

Journal: Biological Trace Element Research

Article Title: The Role of Zinc on Liver Fibrosis by Modulating ZIP14 Expression Throughout Epigenetic Regulatory Mechanisms

doi: 10.1007/s12011-023-04057-5

Figure Lengend Snippet: Changes in mRNA and protein expression of the zinc transporter ZIP14 upon ZnCl 2 treatment in mice with CCl 4 -induced hepatic fibrosis. The Sham group received mineral oil only, CCl 4 and CCl 4 + ZnCl 2 group were injected with 10% CCl 4 in mineral oil for 8 weeks. A 10 µM ZnCl 2 was only injected into the CCl 4 + ZnCl 2 group for two weeks after CCl 4 treatment was completed. A The mRNA expression levels of ZIP14 in were measured by qRT-PCR in hepatocytes. B The protein expression of ZIP14 was measured with flow cytometry and C supported with the immunofluorescence staining with ZIP14 antibody. Hepatocytes were isolated from control (sham) and fibrotic (CCl 4 ) mice were cultured. Transcript levels were normalized to GAPDH. * Indicates p < 0.05, ** indicates p < 0.01. All data are represented as the mean ± SD ( n = 4)

Article Snippet: A 50 μL containing 5 × 10 4 cells as the unstained cell group was separated into a separate microcentrifuge tube and the remaining cells were stained with 1 μL of FITC mouse anti-human ZIP14 (MyBioSource, MBS151580) antibody and isotype antibodies respectively to the cells in 100 μL of 5% BSA and 0.05% sodium azide in 1X PBS.

Techniques: Expressing, Injection, Quantitative RT-PCR, Flow Cytometry, Immunofluorescence, Staining, Isolation, Control, Cell Culture

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Iron increases lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the HIF1α-PPARγ pathway

doi: 10.1007/s00018-022-04423-x

Figure Lengend Snippet: Effects of dietary Fe levels on Fe metabolism in the intestine tissues of yellow catfish. A Prussian blue staining. B Fe content. C, D Protein expression of Fe transport-related genes (DMT1, ZIP14, FPN1 and MTF-1). E The ferritin concentration. F The relative mRNA expression of Ferritin (ftl, ferm and fth). G The relative mRNA expression of genes related to Fe metabolism. Values are shown as means ± SEM (n = 3). Bars that share different letters within the same gene indicate significant differences among the three groups (P < 0.05)

Article Snippet: The membranes were blocked with the TBST with 8% skim milk powder for 1.5 h. Then the membranes were incubated overnight at 4 °C with antibodies against DMT1 (1:1000, A10231, ABclonal, Wuhan, China), Fpn1 (1:1000, A14884, Abclonal, Wuhan, China), GAPDH (1:10000, 10494-1-AP; Proteintech Group, Wuhan, China), HIF1α (1:1000, 20,960–1-AP, Proteintech Group, Wuhan, China), HIF2α (1:1000, 26422-1-AP, Proteintech Group, Wuhan, China), PHD2 (1:1000, 19886-1-AP, Proteintech Group, Wuhan, China), PPARγ (1:1000, 16643-1-AP, Proteintech Group, Wuhan, China), SREBP1 (1:1000, 14088-1-AP, Proteintech Group, Wuhan, China) and ZIP14 (1:1000, A10413, Abclonal, Wuhan, China).

Techniques: Staining, Expressing, Concentration Assay

Journal: Cellular and Molecular Life Sciences: CMLS

Article Title: Iron increases lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the HIF1α-PPARγ pathway

doi: 10.1007/s00018-022-04423-x

Figure Lengend Snippet: DFO attenuated Fe overload-induced Fe2+ accumulation and ROS generation in the primary intestinal epithelial cells (IECs) of yellow catfish. The primary IECs from P. fulvidraco were incubated in the control or Fe (300 μM) for 24 h in DMEM medium with or without 2-h pre-treatment with 100 μM DFO (iron chelator). A Representative fluorescent images of intracellular iron level in IECs were identified by using FerroOrange (orange). Scale bars: 10 μm; B The Fe.2+ ions were quantified by flow cytometric analysis with FerroOrange staining. C Representative confocal microscopic image of ROS stained with DCFH-DA in hepatocytes. D, E The ROS was quantified by flow cytometric analysis with DCFH-DA staining. F, G Western blot and protein level of DMT1, ZIP14 and FPN1. GAPDH was used as internal standard. H MDA content. I Total-AOC. (J) Activity of total-SOD, CAT and GPX. Values are shown as means ± SEM (n = 3). P value was calculated by Student’s t test. *P < 0.05

Article Snippet: The membranes were blocked with the TBST with 8% skim milk powder for 1.5 h. Then the membranes were incubated overnight at 4 °C with antibodies against DMT1 (1:1000, A10231, ABclonal, Wuhan, China), Fpn1 (1:1000, A14884, Abclonal, Wuhan, China), GAPDH (1:10000, 10494-1-AP; Proteintech Group, Wuhan, China), HIF1α (1:1000, 20,960–1-AP, Proteintech Group, Wuhan, China), HIF2α (1:1000, 26422-1-AP, Proteintech Group, Wuhan, China), PHD2 (1:1000, 19886-1-AP, Proteintech Group, Wuhan, China), PPARγ (1:1000, 16643-1-AP, Proteintech Group, Wuhan, China), SREBP1 (1:1000, 14088-1-AP, Proteintech Group, Wuhan, China) and ZIP14 (1:1000, A10413, Abclonal, Wuhan, China).

Techniques: Incubation, Staining, Western Blot, Activity Assay