zic-philic column Search Results


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  • 92
    Merck & Co zic philic column
    Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 448 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA zic philic column
    Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore zic philic column
    Zic Philic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Nest Group sequant zic philic column
    Sequant Zic Philic Column, supplied by The Nest Group, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sequant zic philic column
    Sequant Zic Philic Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co sequant zic philic column
    Sequant Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 89/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA sequant zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 89/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA sequant zic philic polymeric column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Polymeric Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co sequant zic philic guard column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Guard Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Avantor zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Zic Philic Column, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Hichrom Limited zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Zic Philic Column, supplied by Hichrom Limited, used in various techniques. Bioz Stars score: 92/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Nest Group zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Zic Philic Column, supplied by The Nest Group, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co 150 mm sequant zic philic column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    150 Mm Sequant Zic Philic Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sequant zic philic polymeric column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Polymeric Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore zic philic guard column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Zic Philic Guard Column, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA zic philic guard column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Zic Philic Guard Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sequant zic philic guard column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Guard Column, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore sequant zic philic analytical column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Analytical Column, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA sequant zic philic guard column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Guard Column, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck & Co sequant zic philic polymeric column
    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a <t>SeQuant</t> <t>ZIC-pHILIC</t> column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.
    Sequant Zic Philic Polymeric Column, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.

    Journal: Frontiers in Microbiology

    Article Title: Identification and Functional Characterization of Small Alarmone Synthetases in Corynebacterium glutamicum

    doi: 10.3389/fmicb.2017.01601

    Figure Lengend Snippet: In vitro activity characterization of the putative (p)ppGpp synthetases RelP * Cg and RelS Cg , as well as the enzyme Rel Cg . Graphical representation of the HPLC analysis of 50 μL assay reactions containing 500 ng of the corresponding enzyme and ATP+GTP (A) , ATP+GDP (B) and ATP+GMP (C) as substrate combinations with a concentration of 4 mM each. HPLC separation was performed using a SeQuant ZIC-pHILIC column and isocratic elution with 38% of 10 mM ammonium bicarbonate buffer (pH 9.3) and 62% acetonitrile. The reaction products were identified by their specific masses and retention times. Their relative abundance is given as the peak area of the UV signal (252 nm) per mg of the respective enzyme per hour. The results shown were corrected by the values determined for enzyme-free controls. For some measurements pppGpp ( * ) or pGpp ( ** ) were detected by their characteristic MS signals, but the UV detection limits were not reached.

    Article Snippet: HPLC analysis of all assay reactions was performed using a LaChrome ULTRA system (HITACHI) and a SeQuant ZIC-pHILIC column (Merck Millipore).

    Techniques: In Vitro, Activity Assay, High Performance Liquid Chromatography, Concentration Assay, Mass Spectrometry