Journal: Journal of Thoracic Oncology

Article Title: AZGP1 Autoantibody Predicts Survival and Histone Deacetylase Inhibitors Increase Expression in Lung Adenocarcinoma

doi: 10.1097/jto.0b013e318189f5ec

Figure Lengend Snippet: FIGURE 1. Levels of AZGP1 auto- antibody detected in (A) AD and (B) control subjects. The first 140 AD patients showed an autoantibody level of 2 whereas the first 139 control patients showed a level 2. A similar number of patients in each subset showed a level of AZGP1 au- toantibody that was 2. AD, lung adenocarcinoma.

Article Snippet: Immunohistochemical staining was performed on the DAKO Autostainer (DAKO, Carpinteria, CA) using DAKO LSAB and diaminobenzadine as the chromogen, as previously described.9 Deparaffinized sections of the TMA prepared as previously described,10 at 5- m thickness were labeled with AZGP1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, Cat SC-13585, mouse, 1:400) after microwave antigen retrieval in 10 mM sodium citrate buffer, pH 6.

Techniques: Control

Journal: Journal of Thoracic Oncology

Article Title: AZGP1 Autoantibody Predicts Survival and Histone Deacetylase Inhibitors Increase Expression in Lung Adenocarcinoma

doi: 10.1097/jto.0b013e318189f5ec

Figure Lengend Snippet: FIGURE 2. A, Kaplan-Meier survival analysis for AZGP1 au- toantibody in AD patients. Patients with high levels of AZGP1 autoantibody (level median 1.02) had significantly better 5-year survival than patients with low levels of AZGP1 autoantibody (p 0.002). B, Kaplan-Meier survival curve analysis for AZGP1 autoantibody production in patients with stage I and II AD. Patients with high levels (level median 1.02) of autoantibody had significantly better 5-year survival than patients with low levels (p 0.008). AD, lung adeno- carcinoma.

Article Snippet: Immunohistochemical staining was performed on the DAKO Autostainer (DAKO, Carpinteria, CA) using DAKO LSAB and diaminobenzadine as the chromogen, as previously described.9 Deparaffinized sections of the TMA prepared as previously described,10 at 5- m thickness were labeled with AZGP1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, Cat SC-13585, mouse, 1:400) after microwave antigen retrieval in 10 mM sodium citrate buffer, pH 6.

Techniques:

Journal: Journal of Thoracic Oncology

Article Title: AZGP1 Autoantibody Predicts Survival and Histone Deacetylase Inhibitors Increase Expression in Lung Adenocarcinoma

doi: 10.1097/jto.0b013e318189f5ec

Figure Lengend Snippet: FIGURE 3. A, Verification of Kaplan-Meier survival analysis for AZGP1 autoantibody in AD patients. Patients with high levels of AZGP1 autoantibody (level median 1.02) has sig- nificantly better 5-year survival than patients with low levels of autoantibody (p 0.02). B, Verification of Kaplan-Meier survival analysis for AZGP1 autoantibody production in patients with stage I and II AD. Patients with high levels (level median 1.02) of autoantibody had significantly bet- ter 5-year survival than patients with low levels (p 0.036). AD, lung adenocarcinoma.

Article Snippet: Immunohistochemical staining was performed on the DAKO Autostainer (DAKO, Carpinteria, CA) using DAKO LSAB and diaminobenzadine as the chromogen, as previously described.9 Deparaffinized sections of the TMA prepared as previously described,10 at 5- m thickness were labeled with AZGP1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, Cat SC-13585, mouse, 1:400) after microwave antigen retrieval in 10 mM sodium citrate buffer, pH 6.

Techniques:

Journal: Journal of Thoracic Oncology

Article Title: AZGP1 Autoantibody Predicts Survival and Histone Deacetylase Inhibitors Increase Expression in Lung Adenocarcinoma

doi: 10.1097/jto.0b013e318189f5ec

Figure Lengend Snippet: FIGURE 4. Oligonucleotide mi- croarray analysis of 86 AD and 10 normal samples revealed that 31.3% of adenocarcinomas ex- pressed AZGP1 at least two fold more than the controls. Samples 1 to 86, AD; samples 87 to 96, con- trols. AD, lung adenocarcinoma.

Article Snippet: Immunohistochemical staining was performed on the DAKO Autostainer (DAKO, Carpinteria, CA) using DAKO LSAB and diaminobenzadine as the chromogen, as previously described.9 Deparaffinized sections of the TMA prepared as previously described,10 at 5- m thickness were labeled with AZGP1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, Cat SC-13585, mouse, 1:400) after microwave antigen retrieval in 10 mM sodium citrate buffer, pH 6.

Techniques:

Journal: Journal of Thoracic Oncology

Article Title: AZGP1 Autoantibody Predicts Survival and Histone Deacetylase Inhibitors Increase Expression in Lung Adenocarcinoma

doi: 10.1097/jto.0b013e318189f5ec

Figure Lengend Snippet: FIGURE 5. AZGP1 protein staining was not detected in (A) normal lung tissues, but was seen in (B) and (C) 42.8% of AD, and (D) 8.3% of SCC by immunohistochemistry. AD, lung adenocarcinoma; SCC, lung squamous cell carcinoma.

Article Snippet: Immunohistochemical staining was performed on the DAKO Autostainer (DAKO, Carpinteria, CA) using DAKO LSAB and diaminobenzadine as the chromogen, as previously described.9 Deparaffinized sections of the TMA prepared as previously described,10 at 5- m thickness were labeled with AZGP1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, Cat SC-13585, mouse, 1:400) after microwave antigen retrieval in 10 mM sodium citrate buffer, pH 6.

Techniques: Staining, Immunohistochemistry

Journal: Journal of Thoracic Oncology

Article Title: AZGP1 Autoantibody Predicts Survival and Histone Deacetylase Inhibitors Increase Expression in Lung Adenocarcinoma

doi: 10.1097/jto.0b013e318189f5ec

Figure Lengend Snippet: FIGURE 6. A, Western blot demonstrating AZGP1 protein in the serum of all patients, irrespective of AZGP1 protein staining on tissue microarrays. AD, patient with lung adenocarcinoma; N, normal control subject; NE, no AZGP1 protein expression on TMA (negative); WE, weak AZGP1 expression (1); IE, intermediate AZGP1 expression (2); SE, strong AZGP1 expression (3). B, Western blot demonstrating AZGP1 protein in the serum of all patients, irrespective of AZGP1 autoantibody produc- tion. AD, patient with lung adenocarcinoma; N, normal control subject; BL, patient with benign lung disease; Low, patient with serum autoantibody level 2; High, patient with serum autoantibody level 2.

Article Snippet: Immunohistochemical staining was performed on the DAKO Autostainer (DAKO, Carpinteria, CA) using DAKO LSAB and diaminobenzadine as the chromogen, as previously described.9 Deparaffinized sections of the TMA prepared as previously described,10 at 5- m thickness were labeled with AZGP1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, Cat SC-13585, mouse, 1:400) after microwave antigen retrieval in 10 mM sodium citrate buffer, pH 6.

Techniques: Western Blot, Staining, Control, Expressing

Journal: Journal of Thoracic Oncology

Article Title: AZGP1 Autoantibody Predicts Survival and Histone Deacetylase Inhibitors Increase Expression in Lung Adenocarcinoma

doi: 10.1097/jto.0b013e318189f5ec

Figure Lengend Snippet: FIGURE 7. A, AZGP1 mRNA pro- duction is up-regulated in a time dependent manner by HDAC inhibi- tor TSA in A549 cells. Gene pro- moter demethylation by 5-AZA has no effect on AZGP1 mRNA expres- sion in A549 and attenuates the up- regulation by TSA. B, AZGP1 mRNA production is up-regulated in a time dependent manner by HDAC inhibi- tor TSA in SKLU1 cells. Gene pro- moter demethylation by 5-AZA has no effect on AZGP1 mRNA expres- sion in SKLU1 cells and attenuates the up-regulation by TSA. TSA, tri- chostatin A; 5-AZA, 5-aza-2’ deoxy- cytidine. *p 0.05 with t test as compared with control.

Article Snippet: Immunohistochemical staining was performed on the DAKO Autostainer (DAKO, Carpinteria, CA) using DAKO LSAB and diaminobenzadine as the chromogen, as previously described.9 Deparaffinized sections of the TMA prepared as previously described,10 at 5- m thickness were labeled with AZGP1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, Cat SC-13585, mouse, 1:400) after microwave antigen retrieval in 10 mM sodium citrate buffer, pH 6.

Techniques: Control