Journal: Developmental cell

Article Title: Loss of Sirtuin 1 Alters the Secretome of Breast Cancer Cells by Impairing Lysosomal Integrity

doi: 10.1016/j.devcel.2019.03.011

Figure Lengend Snippet: KEY RESOURCE TABLE

Article Snippet: YM155 , Tocris , 6491.

Techniques: Virus, Recombinant, Multiplex sample analysis, Isolation, Luciferase, Cloning, Reverse Transcription, Activity Assay, Control, shRNA, Sequencing, Plasmid Preparation, Software, SYBR Green Assay, Transfection

Journal: Cancers

Article Title: Spironolactone, a Classic Potassium-Sparing Diuretic, Reduces Survivin Expression and Chemosensitizes Cancer Cells to Non-DNA-Damaging Anticancer Drugs

doi: 10.3390/cancers11101550

Figure Lengend Snippet: Spironolactone decreases survivin expression and survivin reductions imitate the chemosensitizing effects of spironolactone. Cells treated with or without 25 µM spironolactone (SPL) for three days were subjected to an immunoblot analysis for survivin expression ( a , b ). YM155, a pharmacological survivin inhibitor, at the concentration of 10 nM and the knockdown of survivin reduced survivin expression in cancer cells (A549) ( c ). The pharmacological or genetic inhibition of survivin sensitized cancer cells (A549) to chemotherapeutic reagents (GEM, gemcitabine, 0.1 µM; OSI, osimertinib, 2 µM) ( d ). * p < 0.05.

Article Snippet: Osimertinib and YM155 were purchased from Chemscene LLC. (Monmouth Junction, NJ, USA) and dissolved in DMSO to 10 mM and 20 μM, respectively, as stock solutions.

Techniques: Expressing, Western Blot, Concentration Assay, Knockdown, Inhibition