Journal: Protein & Cell

Article Title: Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer’s disease mice

doi: 10.1093/procel/pwad056

Figure Lengend Snippet: Social isolation epigenetically disrupts the expression of miRNA in the hippocampal DG area. (A) A representative image for the DG area before (upper) and after (lower) the laser capture microdissection. The nucleus was visualized by Hoechst 33342 (blue). (B) The heatmap for the expression profiles of 40 DG-enriched miRNAs in 7-day SI and group-housed (GH) WT and AD mice. The asterisk indicated Log2 (fold change of miRNA expression) > 0.5 or < −0.5. (C) The expression profiles of 40 DG-enriched miRNAs in the DG of 4-month AD and control mice. The asterisk indicated Log2 (fold change of miRNA expression) > 0.5 or < −0.5. (D) The representative FISH images for the miR-218 and miR-124 in 7 days socially isolated and group-housed AD mice. (E) The expression of has-miR-218 and has-miR-124 in the hippocampus of AD patients. * P < 0.05. (F) The discrimination ratio betweentwo similar contexts [( A − B )/( A + B )] from day 6 to 17 in miR-dDiAs agomirs or scramble treated group-housed or SI AD mice. (G) The average days when the discrimination ratio reaches 0.2 in differently treated AD mice. * P < 0.05. (H) The normalized slope of fEPSP in different groups before (pre-) and after (post-) the HFS. Upper, the representative traces. Lower, the slopes of fEPSP. The arrow indicates the time for HFS. (I) Quantitative analysis of fEPSP slope at last 10 min as indicated by a black line in panel G. *** P < 0.001. (J) The transcriptional levels of host genes for the miR-dDiAs in the DG of 7-day SI and GH WT and AD mice. ** P < 0.01, *** P < 0.001. (K) The CUT&Tag results by using the antibody of H3K27ac. The blue shadow indicated the differentially binding peak. (L) The expression profiles of enzymes that are involved in the histone acetylation and deacetylation detected by qPCR. The asterisk indicated Log2 (fold change of gene expression) >0.4 or <−0.4. (M) The expression levels of miR-dDiAs in the DG neurons of isolated AD mice with the treatment of histone acetyltransferase activator YF-2 or pan-HDAC inhibitor SAHA or vehicle. The asterisk indicated Log2 (fold change of miRNA expression) >1.0 or <−1.0. All results are represented as mean ± SEM.

Article Snippet: Senktide (0.4 mg/kg, s.c. , HY-P0187, MedChemExpress), YF-2 (20 mg/kg, HY-16531, i.p., MedChemExpress), and SAHA (50 mg/kg, HY-10221, i.p., MedChemExpress) were delivered by pipette to mice once per day during 7-day social isolation.

Techniques: Isolation, Expressing, Laser Capture Microdissection, Control, Binding Assay, Gene Expression

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long noncoding RNA LINC00930 promotes PFKFB3-mediated tumor glycolysis and cell proliferation in nasopharyngeal carcinoma

doi: 10.1186/s13046-022-02282-9

Figure Lengend Snippet: LINC00930 promotes NPC progression via PFKFB3. a and b Cell viability and colony formation was assessed after transfection of the PFKFB3 siRNA in LINC00930-overexpressing 6-10B and CNE1 cells. c The ECAR was measured and analyzed after transfection of the the PFKFB3 siRNA in LINC00930-expressing CNE1 cells. d and e Intracellular F-2,6-BP level and lactate level were assayed after transfection of the PFKFB3 siRNA in LINC00930-expressing CNE1 cells. f and g Cell viability and colony formation was assessed after treatment with PFKFB3 inhibitor PFK15 in LINC00930-overexpressing 6-10B and CNE1 cells. h The ECAR was measured and analyzed after treatment with PFKFB3 inhibitor PFK15 in LINC00930-overexpressing CNE1 cells. i and j Intracellular F-2,6-BP level and lactate level were detected after treatment with PFKFB3 inhibitor PFK15 in LINC00930-overexpressing CNE1 cells. k Kaplan-Meier analysis of overall survival of patients in cohort 1, cohort 2, and TCGA HNSC cohort with low (low expression of both LINC00930 and PFKFB3), high (high expression of both LINC00930 and PFKFB3) or intermediate LINC00930/PFKFB3 expression (log-rank test, two-sided). The p -value in a & f was determined by one-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test, no adjustments were made for multiple comparisons. The p -value in b, c, d, e, g, h, i & j was determined by a two-tailed unpaired Student’s t test. * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: YF-2 (#S0022) and PFK15 (#S7289) were obtained from Selleck chemicals.

Techniques: Transfection, Expressing, Two Tailed Test

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Long noncoding RNA LINC00930 promotes PFKFB3-mediated tumor glycolysis and cell proliferation in nasopharyngeal carcinoma

doi: 10.1186/s13046-022-02282-9

Figure Lengend Snippet: Targeting LINC00930 and PFKFB3 in combination with radiotherapy induces tumor regression. a Schematic view of ASO targets on LINC0930 mRNA. b Relative expression of LINC00930 48 h after ASO transfection were determined by qRT-PCR. c qRT-PCR showed LINC00930 expression after ASOs delivery without transfect reagents. ASO were added into 5–8 cells at the concentration as indicated. After 48 h, RNA was extracted, and qRT-PCR was performed. d Cell viability was measured by CCK-8 assay afer irradiation of NPC cells treated with ASO LIN00930 or (and) PFK15. IR, Irradiation. e Schematic view of IR, ASO LINC00930 or (and) PFK15 treatment in CNE2-CDX and NPC-PDX xenograft model. CDX, cell-derived xenograft; PDX, patient-derived xenograft. f and g Statistical analysis of tumor volume in the CNE2-CDX, PDX-1, and PDX-2 models. IR, Irradiation. h Graphical abstract showing that the lncRNA LINC00930 promotes tumor glycolysis and cell proliferation by epigenetically upregulating PFKFB3. The p -value in b & c was determined by a two-tailed unpaired Student’s t test. The p -value in d, f & g was determined by one-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test, no adjustments were made for multiple comparisons. * p < 0.05; ** p < 0.01; *** p < 0.001

Article Snippet: YF-2 (#S0022) and PFK15 (#S7289) were obtained from Selleck chemicals.

Techniques: Expressing, Transfection, Quantitative RT-PCR, Concentration Assay, CCK-8 Assay, Irradiation, Derivative Assay, Two Tailed Test