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    New England Biolabs recombinant yeast xrn1 protein
    <t>XRN1</t> protein preferentially binds to sfRNA in Kunjin or Dengue virus infections. 293T cells were infected with Kunjin virus for 2 d or Dengue virus for 4 d. RNA–protein complexes were stabilized with formaldehyde and immunoprecipitated with XRN1
    Recombinant Yeast Xrn1 Protein, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant yeast xrn1 protein/product/New England Biolabs
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    recombinant yeast xrn1 protein - by Bioz Stars, 2022-12
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    XRN1 protein preferentially binds to sfRNA in Kunjin or Dengue virus infections. 293T cells were infected with Kunjin virus for 2 d or Dengue virus for 4 d. RNA–protein complexes were stabilized with formaldehyde and immunoprecipitated with XRN1

    Journal: RNA

    Article Title: A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability

    doi: 10.1261/rna.034330.112

    Figure Lengend Snippet: XRN1 protein preferentially binds to sfRNA in Kunjin or Dengue virus infections. 293T cells were infected with Kunjin virus for 2 d or Dengue virus for 4 d. RNA–protein complexes were stabilized with formaldehyde and immunoprecipitated with XRN1

    Article Snippet: Approximately 30 fm of radioactive RNAs was incubated in HeLa or C6/36 cytoplasmic S100 extracts prepared as described previously ( ; ) under conditions that strongly favor 5′-to-3′ exonucleolytic decay ( ) or with recombinant yeast XRN1 protein (New England Biolabs) for the times indicated.

    Techniques: Infection, Immunoprecipitation

    Cellular mRNAs are stabilized upon XRN1 depletion. ( A ) XRN1 was depleted in 293T cells using specific XRN1 KD, or cells were treated with control (LKO.1) shRNA vectors. The bar graph illustrates the efficiency of XRN1 knockdown as measured by qRT-PCR.

    Journal: RNA

    Article Title: A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability

    doi: 10.1261/rna.034330.112

    Figure Lengend Snippet: Cellular mRNAs are stabilized upon XRN1 depletion. ( A ) XRN1 was depleted in 293T cells using specific XRN1 KD, or cells were treated with control (LKO.1) shRNA vectors. The bar graph illustrates the efficiency of XRN1 knockdown as measured by qRT-PCR.

    Article Snippet: Approximately 30 fm of radioactive RNAs was incubated in HeLa or C6/36 cytoplasmic S100 extracts prepared as described previously ( ; ) under conditions that strongly favor 5′-to-3′ exonucleolytic decay ( ) or with recombinant yeast XRN1 protein (New England Biolabs) for the times indicated.

    Techniques: shRNA, Quantitative RT-PCR

    sfRNA formation inhibits the cellular exoribonuclease XRN1. A radiolabeled RNA containing a 5′ monophosphate (Reporter) was incubated for the time indicated in HeLa ( A ) or C6/36 ( B ) cytoplasmic extract under conditions that favor 5′-to-3′

    Journal: RNA

    Article Title: A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability

    doi: 10.1261/rna.034330.112

    Figure Lengend Snippet: sfRNA formation inhibits the cellular exoribonuclease XRN1. A radiolabeled RNA containing a 5′ monophosphate (Reporter) was incubated for the time indicated in HeLa ( A ) or C6/36 ( B ) cytoplasmic extract under conditions that favor 5′-to-3′

    Article Snippet: Approximately 30 fm of radioactive RNAs was incubated in HeLa or C6/36 cytoplasmic S100 extracts prepared as described previously ( ; ) under conditions that strongly favor 5′-to-3′ exonucleolytic decay ( ) or with recombinant yeast XRN1 protein (New England Biolabs) for the times indicated.

    Techniques: Incubation

    XRN1 stalls at the 5′ border of the Dengue virus 3′ UTR to generate sfRNAs. Radiolabeled reporter RNAs derived from a pGem4 template (Reporter Only) or RNAs from the same plasmid containing the stem–loop 1 region of the 3′

    Journal: RNA

    Article Title: A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability

    doi: 10.1261/rna.034330.112

    Figure Lengend Snippet: XRN1 stalls at the 5′ border of the Dengue virus 3′ UTR to generate sfRNAs. Radiolabeled reporter RNAs derived from a pGem4 template (Reporter Only) or RNAs from the same plasmid containing the stem–loop 1 region of the 3′

    Article Snippet: Approximately 30 fm of radioactive RNAs was incubated in HeLa or C6/36 cytoplasmic S100 extracts prepared as described previously ( ; ) under conditions that strongly favor 5′-to-3′ exonucleolytic decay ( ) or with recombinant yeast XRN1 protein (New England Biolabs) for the times indicated.

    Techniques: Derivative Assay, Plasmid Preparation

    Uncapped mRNAs accumulate in flavivirus-infected cells due to XRN1 inhibition by sfRNA formation. 293T cells were either mock-treated or infected with Dengue virus (DenV) ( A ), Kunjin virus (KUN) ( B ), or a Kunjin virus mutant that cannot make sfRNA (KUN

    Journal: RNA

    Article Title: A noncoding RNA produced by arthropod-borne flaviviruses inhibits the cellular exoribonuclease XRN1 and alters host mRNA stability

    doi: 10.1261/rna.034330.112

    Figure Lengend Snippet: Uncapped mRNAs accumulate in flavivirus-infected cells due to XRN1 inhibition by sfRNA formation. 293T cells were either mock-treated or infected with Dengue virus (DenV) ( A ), Kunjin virus (KUN) ( B ), or a Kunjin virus mutant that cannot make sfRNA (KUN

    Article Snippet: Approximately 30 fm of radioactive RNAs was incubated in HeLa or C6/36 cytoplasmic S100 extracts prepared as described previously ( ; ) under conditions that strongly favor 5′-to-3′ exonucleolytic decay ( ) or with recombinant yeast XRN1 protein (New England Biolabs) for the times indicated.

    Techniques: Infection, Inhibition, Mutagenesis

    ALPH1ΔN has in vitro decapping activity, in the presence of an RNA 5’ polyphosphatase. Total trypanosome mRNA was treated with recombinant ALPH1 Δ N or Alph1 Δ N* as indicated, purified, treated with RNA 5’ polyphosphatase as indicated, purified, and finally treated with yeast Xrn1 as indicated. The RNA samples were analysed by northern blot probed for the SL RNA (red) and the 5.8S rRNA (green). For lane 4–7 of replicate 1, an overexposed blot of the 5.8S rRNA is shown to demonstrate equal loading. Data of two representative replicates are shown, out of a total of four replicates.

    Journal: PLoS Pathogens

    Article Title: The ApaH-like phosphatase TbALPH1 is the major mRNA decapping enzyme of trypanosomes

    doi: 10.1371/journal.ppat.1006456

    Figure Lengend Snippet: ALPH1ΔN has in vitro decapping activity, in the presence of an RNA 5’ polyphosphatase. Total trypanosome mRNA was treated with recombinant ALPH1 Δ N or Alph1 Δ N* as indicated, purified, treated with RNA 5’ polyphosphatase as indicated, purified, and finally treated with yeast Xrn1 as indicated. The RNA samples were analysed by northern blot probed for the SL RNA (red) and the 5.8S rRNA (green). For lane 4–7 of replicate 1, an overexposed blot of the 5.8S rRNA is shown to demonstrate equal loading. Data of two representative replicates are shown, out of a total of four replicates.

    Article Snippet: To each reaction, 10 μl NEB buffer 3 and 2 μl Ribolock were added and, if required, 2 μl recombinant yeast Xrn1 (NEB).

    Techniques: In Vitro, Activity Assay, Recombinant, Purification, Northern Blot

    ) Dhh1, B) Scd6, C) Xrn1, D) Lsm1-7 complex, E) Dcp1, F) Dcp2, and G) Pat1 C domain with the GST moiety cleaved with PreScission protease. The

    Journal: Molecular cell

    Article Title: Decapping activators in Saccharomyces cerevisiae act by multiple mechanisms

    doi: 10.1016/j.molcel.2010.08.025

    Figure Lengend Snippet: ) Dhh1, B) Scd6, C) Xrn1, D) Lsm1-7 complex, E) Dcp1, F) Dcp2, and G) Pat1 C domain with the GST moiety cleaved with PreScission protease. The

    Article Snippet: Purified yeast Xrn1 was purchased from NEB.

    Techniques: