Journal: International Journal of Molecular Sciences

Article Title: Isolated Lung Perfusion in the Management of Acute Respiratory Distress Syndrome

doi: 10.3390/ijms21186820

Figure Lengend Snippet: Comparison of the two most commonly used EVLP systems.

Article Snippet: While there are a number of ex vivo perfusion systems under investigation, the two most common systems used in clinical practice are the XVIVO Perfusion System (XPS) (XPS Perfusion, Goteborg, Sweden) and the Organ Care System (OCS) (Transmedics, Andover, MA, USA) [ ].

Techniques:

Journal: PLoS ONE

Article Title: Antimicrobial effects of inhaled sphingosine against Pseudomonas aeruginosa in isolated ventilated and perfused pig lungs

doi: 10.1371/journal.pone.0271620

Figure Lengend Snippet: Sphingosine concentrations increased in bronchial epithelial cells after inhalation of a 500 μM solution. EVLP pig lungs were inhaled with sphingosine (n = 5) or saline (0.9% NaCl) as control group (n = 4). Biopsies were fixed in paraformaldehyde, embedded in paraffin and sectioned. Sections were stained with Cy3-coupled anti-sphingosine antibodies. Shown are representative immune stainings for the saline group (A) and the sphingosine group (B) and quantitative analysis of the fluorescence intensity of a total of (C). Given is the mean ± SD of sphingosine fluorescence intensity from each 3 sections with analysis of fluorescence intensity in epithelial cells within 5 visual fields per animal (n = 4 for the control group and n = 5 for the sphingosine group), **p<0.01; students t-test/ Mann-Whitney-u-test.

Article Snippet: In addition, EVLP systems like XVIVO ® built an optimal platform where marginal donor-lungs can easily be subjected and be treated with antibiotics in high dose regimes [ , ] or maybe other anti-infective agents like sphingosine prior to lung re-implantation.

Techniques: Staining, Fluorescence, MANN-WHITNEY

Journal: PLoS ONE

Article Title: Antimicrobial effects of inhaled sphingosine against Pseudomonas aeruginosa in isolated ventilated and perfused pig lungs

doi: 10.1371/journal.pone.0271620

Figure Lengend Snippet: A and B: Quantitative analysis of histological studies from EVLP lungs stained with Cy3-coupled anti-Gr1-antibodies (αGR1). (A) No significant difference in granulocytes and monocytes influx into the epithelial cell layer was observed. Quantitative analysis of the fluorescence intensity of a total of 1200 bronchial epithelial cells per group is displayed. Quantitative analysis of the disruption of epithelial cell layer (B) after inhalation of NaCl 0.9% or 500 μM SPH. No significant differences in epithelial cell integrity using a grading model (Grade 0: no change of the epithelial cell layer, basal membrane intact, less than 2% pyknotic, i.e. dead epithelial cells. Grade 1: small disruptions of the epithelial cell layer, basal membrane intact, less than 5% pyknotic, i.e. dead epithelial cells; Grade 2: Larger disruptions of the epithelial cell layer, basal membrane still intact, less than 10% pyknotic, i.e. dead epithelial cells; Grade 3: Larger disruptions of the epithelial cell layer, disrupted basal membrane, more than 10% pyknotic, i.e. dead epithelial cells) were observed between the groups. Given is the mean ± SD of αGR1 Fluorescence intensity and disruption of epithelial cell layer from each 3 sections with analysis of fluorescence intensity in epithelial cells within 5 visual fields per animal (n = 4 for the control group and n = 5 for the sphingosine group), p<0.05 was considered as significant; students t-test/ Mann-Whitney-u-test.

Article Snippet: In addition, EVLP systems like XVIVO ® built an optimal platform where marginal donor-lungs can easily be subjected and be treated with antibiotics in high dose regimes [ , ] or maybe other anti-infective agents like sphingosine prior to lung re-implantation.

Techniques: Staining, Fluorescence, MANN-WHITNEY

Journal: Nature cardiovascular research

Article Title: The immunobiology and clinical use of genetically engineered porcine hearts for cardiac xenotransplantation

doi: 10.1038/s44161-022-00112-x

Figure Lengend Snippet: The history of cardiac transplantation

Article Snippet: To address PCXD seen with traditional static preservation (that is, slush), a non-ischemic continuous preservation (NICP) system has been developed and commercialized (XVIVO Perfusion) 60 , 88 .

Techniques: Transplantation Assay, Immunopeptidomics, In Vitro, In Vivo, Genetically Modified, Produced, Preserving