Journal: International Journal of Molecular Sciences

Article Title: Generation of a Perfusable 3D Lung Cancer Model by Digital Light Processing

doi: 10.3390/ijms24076071

Figure Lengend Snippet: The 3D model design and perfusion system. ( a ) The 3D model was designed with the Rhino 6 program (left: top view of the model; middle: sectional view of the internal channel of the model; right: front view of the model). The Lumen X DLP bioprinter was then used to produce the 3D model using a GelMA-based bioink. ( b ) The whole perfusion system, including a peristaltic pump connected to the 3D lung cancer model. A side port allows a connection to a syringe. ( c ) The yellow models were perfused with blue ink. After 21 h of perfusion with blue ink, the dark green color indicates a thorough mix of the yellow model and the blue ink. ( d ) Models were then cultured in regular media of orange-red color. Untreated models are shown on the left, while the models on the right were incubated with XTT reagent, which becomes orange when converted by metabolically active cells. The two models on top were cultured under static conditions, while the lower models were perfused. ( e ) Viability of cells in the model after perfusion for four days was quantified by XTT assays. The blank value of the untreated models was subtracted from the models incubated with the reagent. Data are presented as mean ± SD; n = 4. **** p < 0.0001.

Article Snippet: XTT reagent (1 mg/mL in RPMI, Biowest) and phenazine methyl sulfate (PMS, 3.83 mg/mL in PBS, AppliChem, Darmstadt, Germany) were prepared in a 500:1 ratio, and 100 μL were added to the 2D cell culture in each well of a 96-well plate and incubated for 4 h at 37 °C and 5% CO 2 .

Techniques: Cell Culture, Incubation, Metabolic Labelling