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(A) Neurospheres were formed by mixing non-electroporated and electroporated cells before being plated in U-bottom ULA wells. (B) Maximum intensity projection image of a confocal stack of a DIV14 neurosphere in which neurons were separately electroporated with RFP (red) <t>and</t> <t>Xph20-GFP</t> (green). (C) Zoomed images on primary dendrites from neurons expressing intrabodies to PSD-95 (Xph20-GFP), or gephyrin (GPHN.FingR-GFP), revealing excitatory or inhibitory post-synapses, respectively. (D) Numbers of PSD-95 and gephyrin-positive puncta per electroporated neuron. Data represent the mean ± SEM of 14 and 10 neurospheres, respectively, and were compared by non-parametric Mann-Whitney test. Dots show individual neurospheres. (E) Maximum intensity projection of a confocal stack of a DIV14 neurosphere in which neurons were co-electroporated with GPHN.FingR-GFP (green) and Xph20-mRuby2 (magenta), allowing the detection of both excitatory and inhibitory post-synapses in the same cells. (F) Zoom on a dendritic segment corresponding to the rectangular area highlighted in (E). (G) Confocal image of a primary dendrite from a neuron expressing GFP-actin, further immunolabeled for GFP, showing numerous dendritic spines bulging out of the shaft.
Xph20 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xph20+gfp/pCAG_Xph20-eGFP-CCR5TC+(Plasmid+%23135530)/bio_rxiv__64898__2026__03__19__712855-157-4-5
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xph20 gfp - by Bioz Stars, 2026-10
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(A) Neurospheres were formed by mixing non-electroporated and electroporated cells before being plated in U-bottom ULA wells. (B) Maximum intensity projection image of a confocal stack of a DIV14 neurosphere in which neurons were separately electroporated with RFP (red) and Xph20-GFP (green). (C) Zoomed images on primary dendrites from neurons expressing intrabodies to PSD-95 (Xph20-GFP), or gephyrin (GPHN.FingR-GFP), revealing excitatory or inhibitory post-synapses, respectively. (D) Numbers of PSD-95 and gephyrin-positive puncta per electroporated neuron. Data represent the mean ± SEM of 14 and 10 neurospheres, respectively, and were compared by non-parametric Mann-Whitney test. Dots show individual neurospheres. (E) Maximum intensity projection of a confocal stack of a DIV14 neurosphere in which neurons were co-electroporated with GPHN.FingR-GFP (green) and Xph20-mRuby2 (magenta), allowing the detection of both excitatory and inhibitory post-synapses in the same cells. (F) Zoom on a dendritic segment corresponding to the rectangular area highlighted in (E). (G) Confocal image of a primary dendrite from a neuron expressing GFP-actin, further immunolabeled for GFP, showing numerous dendritic spines bulging out of the shaft.

Journal: bioRxiv

Article Title: Neurospheres from primary rodent brain cells to probe the 3D organization and function of synapses

doi: 10.64898/2026.03.19.712855

Figure Lengend Snippet: (A) Neurospheres were formed by mixing non-electroporated and electroporated cells before being plated in U-bottom ULA wells. (B) Maximum intensity projection image of a confocal stack of a DIV14 neurosphere in which neurons were separately electroporated with RFP (red) and Xph20-GFP (green). (C) Zoomed images on primary dendrites from neurons expressing intrabodies to PSD-95 (Xph20-GFP), or gephyrin (GPHN.FingR-GFP), revealing excitatory or inhibitory post-synapses, respectively. (D) Numbers of PSD-95 and gephyrin-positive puncta per electroporated neuron. Data represent the mean ± SEM of 14 and 10 neurospheres, respectively, and were compared by non-parametric Mann-Whitney test. Dots show individual neurospheres. (E) Maximum intensity projection of a confocal stack of a DIV14 neurosphere in which neurons were co-electroporated with GPHN.FingR-GFP (green) and Xph20-mRuby2 (magenta), allowing the detection of both excitatory and inhibitory post-synapses in the same cells. (F) Zoom on a dendritic segment corresponding to the rectangular area highlighted in (E). (G) Confocal image of a primary dendrite from a neuron expressing GFP-actin, further immunolabeled for GFP, showing numerous dendritic spines bulging out of the shaft.

Article Snippet: The intrabody to PSD-95, Xph20-GFP (Addgene #135,530 pCAG_Xph20-eGFP-CCR5TC), was a gift from M. Sainlos (IINS, University of Bordeaux) ( ).

Techniques: Expressing, MANN-WHITNEY, Immunolabeling