Journal: Biotechnology for Biofuels

Article Title: Increased lignocellulosic inhibitor tolerance of Saccharomyces cerevisiae cell populations in early stationary phase

doi: 10.1186/s13068-017-0794-0

Figure Lengend Snippet: Evolution of pHi in different yeast cell inocula (CEN.PK 113-5D expressing pHluorin, TMB3800) upon exposure to a mixture of lignocellulosic inhibitors. Representative histograms from the following inocula: a LP-cells, b cells pre-adapted in medium supplemented with the inhibitors at pH 5, and c ESP-cells. The vertical line displays cells with high and low ratio (F405/F488) and was defined as the number (=0.400) separating LP-cells ( a , inoculum) into fractions of 1% lowest and 99% highest percentiles. d Mean ratio (F405/F488) and e frequency of cells with high ratio (>0.400) for the three different inocula (LP-cells, grey bars ; pre-adapted cells, blue bars ; ESP-cells, green bars ) measured over time. Error bars represent standard error of biological duplicates ( n = 2). Ratio (F405/F488) is the ratio of pHluorin fluorescence emission collected at 542/50 and 533/30 nm originating from excitation at 405 and 488 nm, respectively. Ratiometric flow cytometry measurement was performed with a Moflo cell sorter and data analysis was performed with Flowjo

Article Snippet: A Moflo XPD cell sorter (Becton–Dickinson, NJ, USA) with physically separated laser lines was used for ratiometric flow cytometry analysis of pHluorin fluorescence, as described previously [ ].

Techniques: Expressing, Fluorescence, Flow Cytometry