xpa Search Results


gene exp xpa hs00166045 m1  (Thermo Fisher)
97
Thermo Fisher gene exp xpa hs00166045 m1
Summary of DNA repair genes analyzed with their average fold changes and P -values
Gene Exp Xpa Hs00166045 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xpa  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology xpa
Enhancement of NER protein expression in BRBE-treated HeLa cells. ( A ) <t>XPA,</t> <t>XPB</t> and ( B ) XPD protein expression in HeLa cells, all with and without pre-treatment with BRBE. Tubulin is an inert cellular protein that was used as a control for the overall cellular protein concentrations in BRBE-treated and untreated cells. All Western Blot measurements of relative protein concentrations were conducted within the linear range of overall protein concentrations shown in .
Xpa, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xpa  (Novus Biologicals)
90
Novus Biologicals xpa
Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with <t>specific</t> <t>antibodies</t> against <t>XPA</t> and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.
Xpa, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmids  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology plasmids
Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with <t>specific</t> <t>antibodies</t> against <t>XPA</t> and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.
Plasmids, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna4 flag xpa plasmid  (Addgene inc)
93
Addgene inc pcdna4 flag xpa plasmid
Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with <t>specific</t> <t>antibodies</t> against <t>XPA</t> and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.
Pcdna4 Flag Xpa Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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146 rabbit wb  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc 146 rabbit wb
Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with <t>specific</t> <t>antibodies</t> against <t>XPA</t> and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.
146 Rabbit Wb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xpa  (Biorbyt)
90
Biorbyt xpa
Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with <t>specific</t> <t>antibodies</t> against <t>XPA</t> and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.
Xpa, supplied by Biorbyt, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmids expressing grna targeting xpa  (Santa Cruz Biotechnology)
92
Santa Cruz Biotechnology plasmids expressing grna targeting xpa
Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with <t>specific</t> <t>antibodies</t> against <t>XPA</t> and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.
Plasmids Expressing Grna Targeting Xpa, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xp a  (Bethyl)
92
Bethyl xp a
Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with <t>specific</t> <t>antibodies</t> against <t>XPA</t> and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.
Xp A, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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xab2  (Proteintech)
90
Proteintech xab2
Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with <t>specific</t> <t>antibodies</t> against <t>XPA</t> and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.
Xab2, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti xpa  (Proteintech)
93
Proteintech anti xpa
Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with <t>specific</t> <t>antibodies</t> against <t>XPA</t> and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.
Anti Xpa, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Summary of DNA repair genes analyzed with their average fold changes and P -values

Journal: Lung Cancer

Article Title: Gastroesophageal junction adenocarcinoma displays abnormalities in homologous recombination and nucleotide excision repair

doi: 10.2147/LCTT.S57594

Figure Lengend Snippet: Summary of DNA repair genes analyzed with their average fold changes and P -values

Article Snippet: XPA , Hs00166045_m1 , 0.48 , 0.0004 , 12↓.

Techniques: Homologous Recombination

Enhancement of NER protein expression in BRBE-treated HeLa cells. ( A ) XPA, XPB and ( B ) XPD protein expression in HeLa cells, all with and without pre-treatment with BRBE. Tubulin is an inert cellular protein that was used as a control for the overall cellular protein concentrations in BRBE-treated and untreated cells. All Western Blot measurements of relative protein concentrations were conducted within the linear range of overall protein concentrations shown in .

Journal: Antioxidants

Article Title: Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism

doi: 10.3390/antiox11112110

Figure Lengend Snippet: Enhancement of NER protein expression in BRBE-treated HeLa cells. ( A ) XPA, XPB and ( B ) XPD protein expression in HeLa cells, all with and without pre-treatment with BRBE. Tubulin is an inert cellular protein that was used as a control for the overall cellular protein concentrations in BRBE-treated and untreated cells. All Western Blot measurements of relative protein concentrations were conducted within the linear range of overall protein concentrations shown in .

Article Snippet: The blots were stained with XPA (sc-28353), XPB (sc-271500), anti -XPD (sc-101174), and anti -Tubulin (sc-53140) primary monoclonal antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Expressing, Control, Western Blot

Western Blot signal as a function of tubulin concentration. The range of protein concentration for measuring the XPB, XPA and XPD levels in BRBE-treated and untreated HeLa cells were performed within the linear protein concentration range shown in this graph.

Journal: Antioxidants

Article Title: Treatment of Human HeLa Cells with Black Raspberry Extracts Enhances the Removal of DNA Lesions by the Nucleotide Excision Repair Mechanism

doi: 10.3390/antiox11112110

Figure Lengend Snippet: Western Blot signal as a function of tubulin concentration. The range of protein concentration for measuring the XPB, XPA and XPD levels in BRBE-treated and untreated HeLa cells were performed within the linear protein concentration range shown in this graph.

Article Snippet: The blots were stained with XPA (sc-28353), XPB (sc-271500), anti -XPD (sc-101174), and anti -Tubulin (sc-53140) primary monoclonal antibodies (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Western Blot, Concentration Assay, Protein Concentration

Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with specific antibodies against XPA and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.

Journal: Cancer Epidemiology Biomarkers & Prevention

Article Title: Expression of Nucleotide Excision Repair Proteins in Lymphocytes as a Marker of Susceptibility to Squamous Cell Carcinomas of the Head and Neck

doi: 10.1158/1055-9965.epi-05-0101

Figure Lengend Snippet: Figure 1. Reproducibility and linearity of reverse-protein microarray assay data. A. Serial dilutions of four cell-extract samples (1, 2, 3, and 4) were spotted in triplicate and probed with specific antibodies against XPA and XPF. Each slide was tested three times (repeats 1, 2, and 3). The statistical data are summarized in Table 1. B. The mean intensities of the spots in (A; in arbitrary units) were plotted on a log scale against the number of dilutions of the cell extract. C. Reverse-protein microarray assay of expression of the NER proteins ERCC1, XPA, XPC, XPD, XPF, and XPG. The level of h-actin was used as an internal control for standardization of the expression levels. The statistical data are summarized in Table 3.

Article Snippet: We used mouse anti-human monoclonal or anti-goat or anti-rabbit polyclonal antibodies against XPD and XPG (Santa Cruz Biotechnology, Santa Cruz, CA); XPA, XPC, and XPF (Abcam, Cambridge, MA); ERCC1 (Novus Biological, Littleton, CO); and h-actin (Sigma).

Techniques: Microarray, Expressing, Control