xmai Millipore Search Results


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  • 94
    Millipore kpni
    Kpni, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 613 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/kpni/product/Millipore
    Average 94 stars, based on 613 article reviews
    Price from $9.99 to $1999.99
    kpni - by Bioz Stars, 2020-07
    94/100 stars
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    85
    Millipore ncoi xmai opened pyx242
    Ncoi Xmai Opened Pyx242, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ncoi xmai opened pyx242/product/Millipore
    Average 85 stars, based on 8 article reviews
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    99
    Millipore hindiii
    Hindiii, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2796 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2796 article reviews
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    hindiii - by Bioz Stars, 2020-07
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    97
    Millipore pflag cmv2 vector
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Pflag Cmv2 Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pflag cmv2 vector/product/Millipore
    Average 97 stars, based on 545 article reviews
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    88
    Millipore pet47b
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Pet47b, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet47b/product/Millipore
    Average 88 stars, based on 178 article reviews
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    90
    Thermo Fisher pdisplay
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Pdisplay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 687 article reviews
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    pdisplay - by Bioz Stars, 2020-07
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    88
    Millipore pacycduet
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Pacycduet, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 144 article reviews
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    pacycduet - by Bioz Stars, 2020-07
    88/100 stars
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    91
    TaKaRa ecori xmai
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Ecori Xmai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ecori xmai - by Bioz Stars, 2020-07
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    99
    Millipore xhoi
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Xhoi, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5009 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 5009 article reviews
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    xhoi - by Bioz Stars, 2020-07
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    88
    Millipore pet27b
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Pet27b, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 145 article reviews
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    pet27b - by Bioz Stars, 2020-07
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    99
    Millipore microcon spin columns
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Microcon Spin Columns, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore plasmid dna
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Plasmid Dna, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7013 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore genejuice
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Genejuice, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 4116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore pet32a
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Pet32a, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2499 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 2499 article reviews
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    99
    Millipore ndei
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Ndei, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13502 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore ptriex 4 neo vector
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Ptriex 4 Neo Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Merck KGaA pflag cmv2
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Pflag Cmv2, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pflag cmv2/product/Merck KGaA
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    88
    Millipore sacii restriction sites
    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in <t>pFLAG-CMV2</t> plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.
    Sacii Restriction Sites, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in pFLAG-CMV2 plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.

    Journal: eLife

    Article Title: LARP4 mRNA codon-tRNA match contributes to LARP4 activity for ribosomal protein mRNA poly(A) tail length protection

    doi: 10.7554/eLife.28889

    Figure Lengend Snippet: LARP4 mRNA contains a codon-specific, coding region determinant (CRD) of instability in that limits expression. ( a ) Schematic showing expression cassette in pFLAG-CMV2 plasmids that differ only in the open reading frame (ORF) coding sequences (CDS). ( b ) Western blot using anti-FLAG antibody; arrow indicates LARP4 band, lane 4. ( c ) Northern blot, upper panel shows detection by FLAG-Hind antisense probe. VA1 RNA was for transfection control and quantification. ( d ) Normalized expression of three experiments (n = 3), error bars represent s.e.m.; Flag-LARP4 set to 1. ( e ) Schematic of LARP4 mutated constructs transfected with VA1 for f and g. ( f ) Upper: Western blot with anti-FLAG and anti-GAPDH. Lower: Northern blot with FLAG-Hind antisense and VA1 probes. ( g ) Upper: Western blot with anti -FLAG, -actin and -GFP antibodies. Lower: Northern for FLAG-Hind and VA1. ( h ) Schematic showing two β-globin reporters containing CRD constructs (see text); 'in 3’UTR' following the stop codon, and in frame preceding the stop 'in CDS'. ( i ) Northern blot time course of decay of the β-glo CRD reporter mRNAs. WT = wild type CRD sequence and CS = synonymous codon swapped version of the CRD. ( j ) Quantification of β-glo mRNA in i; 3 independent experiments, error bars represent s.e.m. GFP used for normalization, and each t = 0 was set to 100%. ( k ) Similar to i; +2 FS= + 2 frameshift version of the WT CRD.

    Article Snippet: After restriction digestion with KpnI, HindIII and XmaI, the CDS and 3’UTR were ligated and inserted into HindIII and XmaI sites of pFlag-CMV2 vector (Sigma-Aldrich).

    Techniques: Expressing, Western Blot, Northern Blot, Transfection, Construct, Sequencing

    Genome-wide mapping of Z-DNA using Zaa. (A) Schematic of Zaa with FLAG tag and SV40NLS. (B) The localization of Zaa in HeLa cells was observed by confocal microscopy. Zaa was labeled with FLAG antibody, followed by a secondary antibody conjugated to green-fluorescent dye. Nuclei were stained using Hoechst dye (blue). Pictures are representative of three independent experiments. Scale bars indicate 10 μm. The Western blot image is shown on the right panel. (C) Flowchart illustrating the ChIP-Seq process. Zaa construct was transiently transfected into HeLa cells. (D) Circos plot displaying the genome-wide distribution of genes, ZFSs, and polymerase II (gray bar: RefSeq gene, blue bar: ZFSs, orange bar: RNA polymerase II peak). One representative region in chromosome 1 is shown on the UCSC genome browser. The y-axis of the UCSC genome browser is the normalized read count. Peak regions, ZFS, and predicted ZDRs in ZFSs (cutoff of − 0.08) are indicated as gray bars, blue bars, and pink bars, respectively. (E) Examples of ZFSs are shown. The UCSC genome browser was used to visualize read distributions of three Zaa replicates, IgG, and input in ANKRD11, SRSF6 , and SIK1 . The PCR primer positions for ChIP validation are marked by big arrow.

    Journal: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes

    Article Title: Z-DNA-forming sites identified by ChIP-Seq are associated with actively transcribed regions in the human genome

    doi: 10.1093/dnares/dsw031

    Figure Lengend Snippet: Genome-wide mapping of Z-DNA using Zaa. (A) Schematic of Zaa with FLAG tag and SV40NLS. (B) The localization of Zaa in HeLa cells was observed by confocal microscopy. Zaa was labeled with FLAG antibody, followed by a secondary antibody conjugated to green-fluorescent dye. Nuclei were stained using Hoechst dye (blue). Pictures are representative of three independent experiments. Scale bars indicate 10 μm. The Western blot image is shown on the right panel. (C) Flowchart illustrating the ChIP-Seq process. Zaa construct was transiently transfected into HeLa cells. (D) Circos plot displaying the genome-wide distribution of genes, ZFSs, and polymerase II (gray bar: RefSeq gene, blue bar: ZFSs, orange bar: RNA polymerase II peak). One representative region in chromosome 1 is shown on the UCSC genome browser. The y-axis of the UCSC genome browser is the normalized read count. Peak regions, ZFS, and predicted ZDRs in ZFSs (cutoff of − 0.08) are indicated as gray bars, blue bars, and pink bars, respectively. (E) Examples of ZFSs are shown. The UCSC genome browser was used to visualize read distributions of three Zaa replicates, IgG, and input in ANKRD11, SRSF6 , and SIK1 . The PCR primer positions for ChIP validation are marked by big arrow.

    Article Snippet: The SV40 nuclear localization signal (NLS) sequence was generated as described and inserted into the XmaI/AscI site of pEF1α, producing pEF1α-Zaa. pEF1α-Zaa was transfected into HeLa cells using GeneJuice (Novagen, Germany).

    Techniques: Genome Wide, FLAG-tag, Confocal Microscopy, Labeling, Staining, Western Blot, Chromatin Immunoprecipitation, Construct, Transfection, Polymerase Chain Reaction