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  • 99
    New England Biolabs xmai
    Transgenerational CRISPR-Cas9 activity induces new mutations in the TaGW2 and TaLpx-1 genes. NGS reads flanking the GW2T2 target site and their frequencies in (A) T 0 line GLM-2, (B) T 1 line GLM-2-9, and (C) T 2 line GLM-2-9-49 are shown. (D) Restriction enzyme digestion of polymerase chain reaction <t>(PCR)</t> amplicons to screen gw2 knockout mutations in the T 3 progenies of line GLM-2-9-49. The GW2T2 flanking region was amplified by PCR and digested with <t>XmaI;</t> non-digested PCR amplicons correspond to mutated GW2T2 target sites. The numbers on the gel image are identifiers of the GLM-2-9-49 progenies. Lanes marked with arrows are PCR products from wild-type plant not digested with XmaI and loaded as controls; the knockout mutant plant was marked with a star. BW, wild-type cultivar Bobwhite. (E) Sanger sequencing of PCR-amplified GW2T2 target sites of T 3 line GLM-2-9-49-28. Genome specific primers were used to amplify regions flanking the GW2T2 target sites. Nucleotide substitutions are marked with red rectangles, and the inserted nucleotide is shown by the red arrow. Types and frequencies of mutations at the GW2T2, LPX1T2, and MLOT1 target sites in (F) T 1 line GLM-2-5, and (G) T 2 line GLM-2-5-24 are shown. WT, wild-type alleles in wheat cultivar Bobwhite; “–” and “+” signs and numbers after them, nucleotides deleted and inserted, respectively. The frequency of each mutation type is shown on the right. The PAM sequences are underlined; the deleted nucleotides are shown with red dashed lines; the insertions and deletions are highlighted in red.
    Xmai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher cfr 9i
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Cfr 9i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega xmai
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Xmai, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Genewiz xmai
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Xmai, supplied by Genewiz, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher xmai
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Xmai, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 255 article reviews
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    xmai - by Bioz Stars, 2020-08
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    93
    Thermo Fisher xmai bsmi sites
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Xmai Bsmi Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    New England Biolabs xmai bstbi
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Xmai Bstbi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    SibEnzyme xmai
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Xmai, supplied by SibEnzyme, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega xmai restriction enzymes
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Xmai Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Retrogen xmai
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Xmai, supplied by Retrogen, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa ecori xmai blunted peyfp n1
    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and <t>Cfr</t> 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.
    Ecori Xmai Blunted Peyfp N1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Transgenerational CRISPR-Cas9 activity induces new mutations in the TaGW2 and TaLpx-1 genes. NGS reads flanking the GW2T2 target site and their frequencies in (A) T 0 line GLM-2, (B) T 1 line GLM-2-9, and (C) T 2 line GLM-2-9-49 are shown. (D) Restriction enzyme digestion of polymerase chain reaction (PCR) amplicons to screen gw2 knockout mutations in the T 3 progenies of line GLM-2-9-49. The GW2T2 flanking region was amplified by PCR and digested with XmaI; non-digested PCR amplicons correspond to mutated GW2T2 target sites. The numbers on the gel image are identifiers of the GLM-2-9-49 progenies. Lanes marked with arrows are PCR products from wild-type plant not digested with XmaI and loaded as controls; the knockout mutant plant was marked with a star. BW, wild-type cultivar Bobwhite. (E) Sanger sequencing of PCR-amplified GW2T2 target sites of T 3 line GLM-2-9-49-28. Genome specific primers were used to amplify regions flanking the GW2T2 target sites. Nucleotide substitutions are marked with red rectangles, and the inserted nucleotide is shown by the red arrow. Types and frequencies of mutations at the GW2T2, LPX1T2, and MLOT1 target sites in (F) T 1 line GLM-2-5, and (G) T 2 line GLM-2-5-24 are shown. WT, wild-type alleles in wheat cultivar Bobwhite; “–” and “+” signs and numbers after them, nucleotides deleted and inserted, respectively. The frequency of each mutation type is shown on the right. The PAM sequences are underlined; the deleted nucleotides are shown with red dashed lines; the insertions and deletions are highlighted in red.

    Journal: The Crispr Journal

    Article Title: Transgenerational CRISPR-Cas9 Activity Facilitates Multiplex Gene Editing in Allopolyploid Wheat

    doi: 10.1089/crispr.2017.0010

    Figure Lengend Snippet: Transgenerational CRISPR-Cas9 activity induces new mutations in the TaGW2 and TaLpx-1 genes. NGS reads flanking the GW2T2 target site and their frequencies in (A) T 0 line GLM-2, (B) T 1 line GLM-2-9, and (C) T 2 line GLM-2-9-49 are shown. (D) Restriction enzyme digestion of polymerase chain reaction (PCR) amplicons to screen gw2 knockout mutations in the T 3 progenies of line GLM-2-9-49. The GW2T2 flanking region was amplified by PCR and digested with XmaI; non-digested PCR amplicons correspond to mutated GW2T2 target sites. The numbers on the gel image are identifiers of the GLM-2-9-49 progenies. Lanes marked with arrows are PCR products from wild-type plant not digested with XmaI and loaded as controls; the knockout mutant plant was marked with a star. BW, wild-type cultivar Bobwhite. (E) Sanger sequencing of PCR-amplified GW2T2 target sites of T 3 line GLM-2-9-49-28. Genome specific primers were used to amplify regions flanking the GW2T2 target sites. Nucleotide substitutions are marked with red rectangles, and the inserted nucleotide is shown by the red arrow. Types and frequencies of mutations at the GW2T2, LPX1T2, and MLOT1 target sites in (F) T 1 line GLM-2-5, and (G) T 2 line GLM-2-5-24 are shown. WT, wild-type alleles in wheat cultivar Bobwhite; “–” and “+” signs and numbers after them, nucleotides deleted and inserted, respectively. The frequency of each mutation type is shown on the right. The PAM sequences are underlined; the deleted nucleotides are shown with red dashed lines; the insertions and deletions are highlighted in red.

    Article Snippet: To screen the gw2 knockout mutants, the GW2T2 target region from all three homoeologs was amplified, and PCR products were digested with XmaI (NEB).

    Techniques: CRISPR, Activity Assay, Next-Generation Sequencing, Polymerase Chain Reaction, Knock-Out, Amplification, Mutagenesis, Sequencing

    Transgene expression cassettes and Southern analysis of selected transgenic lines. a RGA2 and b Ced9 expression cassettes. LB, left border; RB, right border. Determination of transgene copy number in c RGA2 and d Ced9 transgenic banana lines by Southern blot analysis. Genomic DNA from WT, RGA2 and Ced9 lines was digested with Hin dIII and Xma I, respectively. DNA molecular weight marker II (Roche) reference is indicated on the right hand side

    Journal: Nature Communications

    Article Title: Transgenic Cavendish bananas with resistance to Fusarium wilt tropical race 4

    doi: 10.1038/s41467-017-01670-6

    Figure Lengend Snippet: Transgene expression cassettes and Southern analysis of selected transgenic lines. a RGA2 and b Ced9 expression cassettes. LB, left border; RB, right border. Determination of transgene copy number in c RGA2 and d Ced9 transgenic banana lines by Southern blot analysis. Genomic DNA from WT, RGA2 and Ced9 lines was digested with Hin dIII and Xma I, respectively. DNA molecular weight marker II (Roche) reference is indicated on the right hand side

    Article Snippet: Southern blot analysis For determination of transgene copy number integration by Southern analysis, total nucleic acid was extracted from banana leaf tissue, treated with RNAse A, and 15 μg aliquots of genomic DNA were digested overnight with 20 U of restriction enzyme Hin dIII or Xma I (New England Biolabs) overnight at 37 °C.

    Techniques: Expressing, Transgenic Assay, Southern Blot, Molecular Weight, Marker

    Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and Cfr 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.

    Journal: Microbial Drug Resistance

    Article Title: Changes in Macrolide Resistance Among Group A Streptococci in Serbia and Clonal Evolution of Resistant Isolates

    doi: 10.1089/mdr.2017.0306

    Figure Lengend Snippet: Dendrogram showing the genetic relationship of 50 macrolide-resistant Streptococcus pyogenes established from PFGE patterns obtained after Sma I and Cfr 9I digestion and their antibiotic resistance gene content. The dendrogram, to the left, compares the percent of genetic relatedness among resistant isolates. The level at which the vertical line transects the horizontal line from the PFGE of each isolate determines its similarity based on the percent scale above the dendrogram. PFGE clusters were defined as isolates sharing at least 80% similarity. Each major lineage is designated by a capital letter. PFGE, pulsed-field gel electrophoresis.

    Article Snippet: Briefly, chromosomal DNAs of MRGAS isolates expressing the MLS phenotype were digested with the Sma I (10 U) restriction enzyme (Thermo Scientific), (run time: 18 hr, switching intervals: 5–35 sec, angle: 120°, voltage: 6 V, and cooling temperature: 14°C), while DNAs of strains with an M phenotype were treated with the Cfr 9I (30 U) restriction enzyme (Thermo Scientific), (run time: 18 hr, switching intervals: 5–35 sec, angle: 120°, voltage: 6 V, and cooling temperature: 14°C).

    Techniques: Pulsed-Field Gel, Electrophoresis