xhoi Thermo Fisher Search Results


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  • 95
    Millipore xhoi
    Xhoi, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 4100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher xho i
    (A) Digestion of pTY-α-amylase with <t>Nhe</t> I and <t>Xho</t> I restriction enzymes. Lane 1: pTYB2 digested with Nhe I and Xho I; lane2: pTY-α-amylase digested with Nhe I and Xho I; lane 3: PCR product of α-amylase. (B) SDS-PAGE analysis of α-amylase expression. Lane 1: before IPTG induction; lane 2: after IPTG induction; lane 3: 16 hours after IPTG induction; lane 4: 20 hours after IPTG induction. (C) Western blot analysis of α-amylase with anti-intein polyclonal antibody. Lane1: α-amylase; lane 2: lysate of untransformed bacteria. IPTG = isopropyl- β-d-thiogalactopyranoside; M = molecular marker; SDS-PAGE = sodium dodecyl sulfate polyacrylamide gel electrophoresis.
    Xho I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4040 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher fastdigest xhoi
    (A) Digestion of pTY-α-amylase with <t>Nhe</t> I and <t>Xho</t> I restriction enzymes. Lane 1: pTYB2 digested with Nhe I and Xho I; lane2: pTY-α-amylase digested with Nhe I and Xho I; lane 3: PCR product of α-amylase. (B) SDS-PAGE analysis of α-amylase expression. Lane 1: before IPTG induction; lane 2: after IPTG induction; lane 3: 16 hours after IPTG induction; lane 4: 20 hours after IPTG induction. (C) Western blot analysis of α-amylase with anti-intein polyclonal antibody. Lane1: α-amylase; lane 2: lysate of untransformed bacteria. IPTG = isopropyl- β-d-thiogalactopyranoside; M = molecular marker; SDS-PAGE = sodium dodecyl sulfate polyacrylamide gel electrophoresis.
    Fastdigest Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher xhoi
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher ecori xhoi
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Ecori Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher bamhi xhoi
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Bamhi Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 214 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher slp773 xhoi re
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Slp773 Xhoi Re, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher xhoi noti
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Xhoi Noti, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher pgex6p 2
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Pgex6p 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher xhoi pmei digested pyd1
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Xhoi Pmei Digested Pyd1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher ptrchis
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Ptrchis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 281 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher xhoi hindiii
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Xhoi Hindiii, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher pacv5 hisa
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Pacv5 Hisa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher xhoi xbai sites
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Xhoi Xbai Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher restriction enzyme xhoi
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Restriction Enzyme Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher ecori xhoi digested pcr3 1
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Ecori Xhoi Digested Pcr3 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher ncoi xhoi restricted pfastbac hta
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Ncoi Xhoi Restricted Pfastbac Hta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher pcdna 3 1
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Pcdna 3 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 5383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher psti xhoi
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Psti Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher xhoi digested pcdna3 1
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Xhoi Digested Pcdna3 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher xhoi cleaved pcdna6 myc hisa
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Xhoi Cleaved Pcdna6 Myc Hisa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Thermo Fisher xhoi digested pcdna6 a myc his
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Xhoi Digested Pcdna6 A Myc His, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher kpni xhoi opened pbluescript ii sk
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Kpni Xhoi Opened Pbluescript Ii Sk, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher 5 xhoi 3 paci restriction sites
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    5 Xhoi 3 Paci Restriction Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher pcdna3 hisb
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Pcdna3 Hisb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher xhoi linearized pcep4
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Xhoi Linearized Pcep4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher nhei xhoi cleaved pcep4
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Nhei Xhoi Cleaved Pcep4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    xho  (TaKaRa)
    90
    TaKaRa xho
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Xho, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher hindiii xhoi restriction
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
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    80
    Thermo Fisher ecorv xhoi
    <t>pJ915-rhChymase</t> vector map The P. pastoris ] using 5’ <t>XhoI</t> and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.
    Ecorv Xhoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    (A) Digestion of pTY-α-amylase with Nhe I and Xho I restriction enzymes. Lane 1: pTYB2 digested with Nhe I and Xho I; lane2: pTY-α-amylase digested with Nhe I and Xho I; lane 3: PCR product of α-amylase. (B) SDS-PAGE analysis of α-amylase expression. Lane 1: before IPTG induction; lane 2: after IPTG induction; lane 3: 16 hours after IPTG induction; lane 4: 20 hours after IPTG induction. (C) Western blot analysis of α-amylase with anti-intein polyclonal antibody. Lane1: α-amylase; lane 2: lysate of untransformed bacteria. IPTG = isopropyl- β-d-thiogalactopyranoside; M = molecular marker; SDS-PAGE = sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Journal: Osong Public Health and Research Perspectives

    Article Title: Cloning, Expression, and Purification of Hyperthermophile α-Amylase from Pyrococcus woesei

    doi: 10.1016/j.phrp.2015.10.003

    Figure Lengend Snippet: (A) Digestion of pTY-α-amylase with Nhe I and Xho I restriction enzymes. Lane 1: pTYB2 digested with Nhe I and Xho I; lane2: pTY-α-amylase digested with Nhe I and Xho I; lane 3: PCR product of α-amylase. (B) SDS-PAGE analysis of α-amylase expression. Lane 1: before IPTG induction; lane 2: after IPTG induction; lane 3: 16 hours after IPTG induction; lane 4: 20 hours after IPTG induction. (C) Western blot analysis of α-amylase with anti-intein polyclonal antibody. Lane1: α-amylase; lane 2: lysate of untransformed bacteria. IPTG = isopropyl- β-d-thiogalactopyranoside; M = molecular marker; SDS-PAGE = sodium dodecyl sulfate polyacrylamide gel electrophoresis.

    Article Snippet: P. woesei chromosomal DNA was used as a source of the α-amylase gene and polymerase chain reaction (PCR) amplification was done with the following primers: 5′- GCTAGC TTGGAGCTTGAAGAGGGAG-3′ and 5′- GAGACC ACAATAACTCCATACGGAG-3′ containing recognition sites for restriction endonucleases Nhe I and Xho I (Fermentas; Vilnius, Lithuania).

    Techniques: Polymerase Chain Reaction, SDS Page, Expressing, Western Blot, Marker, Polyacrylamide Gel Electrophoresis

    Graphical representation of pET28a-bglA. Expression vector was created by insertion of bglA gene between NcoI and XhoI sites. Inserted sequence was adjacent to lac operator and terminates at 6xHis-tag included in the frame. Kan R : kanamycin resistance gene, Ori: origin of replication. The sketch was created using SnapGene® software (GSL Biotech).

    Journal: BioMed Research International

    Article Title: Soluble Production, Characterization, and Structural Aesthetics of an Industrially Important Thermostable β-Glucosidase from Clostridium thermocellum in Escherichia coli

    doi: 10.1155/2019/9308593

    Figure Lengend Snippet: Graphical representation of pET28a-bglA. Expression vector was created by insertion of bglA gene between NcoI and XhoI sites. Inserted sequence was adjacent to lac operator and terminates at 6xHis-tag included in the frame. Kan R : kanamycin resistance gene, Ori: origin of replication. The sketch was created using SnapGene® software (GSL Biotech).

    Article Snippet: NcoI and XhoI (Fermentas Inc., Ontario, Canada) restriction enzymes were used for restriction of cloning and expression vectors.

    Techniques: Expressing, Plasmid Preparation, Sequencing, Software

    pJ915-rhChymase vector map The P. pastoris ] using 5’ XhoI and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.

    Journal: Protein expression and purification

    Article Title: Expression of Recombinant Human Mast Cell Chymase with Asn-linked Glycans in Glycoengineered Pichia pastoris

    doi: 10.1016/j.pep.2014.08.005

    Figure Lengend Snippet: pJ915-rhChymase vector map The P. pastoris ] using 5’ XhoI and 3’ NotI restriction sites. The GAP promoter provides constitutive expression of the fusion protein and the plasmid contains a Zeocin resistance selectable marker gene. The plasmid is linearized at the SwaI restriction site prior to electroporation.

    Article Snippet: The DNA encoding human Chymase (hChymase) with an added N-terminal Kex2 protease cleavage site was isolated from the previously described plasmid pPICzα-rhChymase [ ] using simultaneous digestion with XhoI and NotI restriction endonucleases (Thermo Scientific, FastDigest).

    Techniques: Plasmid Preparation, Expressing, Marker, Electroporation