Journal: Frontiers in Plant Science
Article Title: A dehydrin gene isolated from feral olive enhances drought tolerance in Arabidopsis transgenic plants
Figure Lengend Snippet: OesDHN genomic organization. (A) Scheme of OesDHN gene. The black rectangle indicates intron position with respect to the cDNA. Start and stop codons are typed. (C) Deduced nucleotides of OesDHN cDNA and its 5′ upstream region and features of Oes DHN protein. The ATG start and TAA stop codons are indicated in bold; the stop codon position is indicated by the asterisk. The intron sequence, including the putative TATA-box (bold) is highlighted. The putative ABRE, G-box, MYB, GATA, CAAT-box elements are also indicated. Predicted amino acids are shown in one letter code. The S-segment is shown with an interrupted line, and the two K-segments are shown in bold italics and underlined with a single line. The putative NLS (nuclear localization signal) is double underlined and the poly-proline cluster is shown with one single line. The intronic region is represented with italic lowercases. (B) Southern blot analysis. The oleaster gDNA was digested with EcoRI and XbaI and hybridized with the cDNA probe, indicated in (A) . The molecular weights of a co-migrating DNA marker are expressed in kilo base pairs (Kb). PR, promoter region; UTR, Untranslated region. Bar 200 bp (A) .
Article Snippet: Briefly, after extraction and purification, gDNA (10 μg) was digested overnight at 37°C with EcoRV and XbaI endonucleases (Promega, Italy), which do not cut in the probe.
Techniques: Sequencing, Southern Blot, Marker