x2 Search Results


transit x2  (Mirus Bio)
98
Mirus Bio transit x2
Transit X2, supplied by Mirus Bio, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/transit x2/product/Mirus Bio
Average 98 stars, based on 1 article reviews
transit x2 - by Bioz Stars, 2026-05
98/100 stars
  Buy from Supplier
automated particle shape analysis  (Verder Scientific)
96
Verder Scientific automated particle shape analysis
Automated Particle Shape Analysis, supplied by Verder Scientific, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/automated particle shape analysis/product/Verder Scientific
Average 96 stars, based on 1 article reviews
automated particle shape analysis - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
pcag nuclear localization signal nls gfp  (Addgene inc)
91
Addgene inc pcag nuclear localization signal nls gfp
Pcag Nuclear Localization Signal Nls Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcag nuclear localization signal nls gfp/product/Addgene inc
Average 91 stars, based on 1 article reviews
pcag nuclear localization signal nls gfp - by Bioz Stars, 2026-05
91/100 stars
  Buy from Supplier
analytical grade anion exchange resin beads agi x2  (Bio-Rad)
94
Bio-Rad analytical grade anion exchange resin beads agi x2
Analytical Grade Anion Exchange Resin Beads Agi X2, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/analytical grade anion exchange resin beads agi x2/product/Bio-Rad
Average 94 stars, based on 1 article reviews
analytical grade anion exchange resin beads agi x2 - by Bioz Stars, 2026-05
94/100 stars
  Buy from Supplier
hno 3  (Revvity)
91
Revvity hno 3
Hno 3, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hno 3/product/Revvity
Average 91 stars, based on 1 article reviews
hno 3 - by Bioz Stars, 2026-05
91/100 stars
  Buy from Supplier
mastercycler nexus x2 thermocycler  (Eppendorf AG)
95
Eppendorf AG mastercycler nexus x2 thermocycler
Mastercycler Nexus X2 Thermocycler, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mastercycler nexus x2 thermocycler/product/Eppendorf AG
Average 95 stars, based on 1 article reviews
mastercycler nexus x2 thermocycler - by Bioz Stars, 2026-05
95/100 stars
  Buy from Supplier
ronald cohn  (Addgene inc)
93
Addgene inc ronald cohn
Ronald Cohn, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ronald cohn/product/Addgene inc
Average 93 stars, based on 1 article reviews
ronald cohn - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
50w x2 resin  (Bio-Rad)
93
Bio-Rad 50w x2 resin
50w X2 Resin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/50w x2 resin/product/Bio-Rad
Average 93 stars, based on 1 article reviews
50w x2 resin - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
hgfr met  (Addgene inc)
90
Addgene inc hgfr met
Figure 6. Deletion of Pericyte-b3-Integrin Enhances Tumor Cell Growth via Pericyte-pAkt-p65-Regulated Cytokine Production (A) Western blot analysis of b3-null pericytes with CRISPR-Cas9 deletion <t>of</t> <t>Akt1</t> (b3-null;AktKO PC) or <t>HGFR</t> (b3-null;HGFRKO PC) compared with b3-null;Cas9 controls. Hsc70, loading control. Blots representative of 3 separate experiments. Bar charts, average densitometric analysis relative to loading controls (n = 3 experimental repeats). (B) B16F0 cell survival after exposure to conditioned media from b3-null;AktKO, b3-null;HGFRKO, or b3-null;Cas9 pericytes (n = 16 technical replicates for 2 separate experiments). (C) Tumor growth in WT mice injected subcutaneously with either B16F0 cells plus b3-null;Cas9 pericytes or B16F0 plus b3-null;AktKO pericytes at 1:8 ratio (n = 10 per group). (D) Western blot analysis using mock- and Ik-Ba-SR-transfected WT or b3-null pericyte lysates. HSC70, loading control. (E) Protein-profiler cytokine arrays. Quantitation of fold difference in cytokine expression between mock- and Ik-Ba-SR-transfected b3-null pericytes (n = 4 dots from 2 independent experiments). (F) Conditioned media from mock- and Ik-Ba-SR-transfected b3-null pericytes were applied to B16F0, B16F10, and LLC cells and cell survival measured by MTS assay. Bar charts, means ± SEM relative to tumor cells treated with conditioned media from mock-transfected b3-null pericytes (n = 3 experimental repeats).
Hgfr Met, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hgfr met/product/Addgene inc
Average 90 stars, based on 1 article reviews
hgfr met - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
addgene plasmid  (Addgene inc)
93
Addgene inc addgene plasmid
Figure 6. Deletion of Pericyte-b3-Integrin Enhances Tumor Cell Growth via Pericyte-pAkt-p65-Regulated Cytokine Production (A) Western blot analysis of b3-null pericytes with CRISPR-Cas9 deletion <t>of</t> <t>Akt1</t> (b3-null;AktKO PC) or <t>HGFR</t> (b3-null;HGFRKO PC) compared with b3-null;Cas9 controls. Hsc70, loading control. Blots representative of 3 separate experiments. Bar charts, average densitometric analysis relative to loading controls (n = 3 experimental repeats). (B) B16F0 cell survival after exposure to conditioned media from b3-null;AktKO, b3-null;HGFRKO, or b3-null;Cas9 pericytes (n = 16 technical replicates for 2 separate experiments). (C) Tumor growth in WT mice injected subcutaneously with either B16F0 cells plus b3-null;Cas9 pericytes or B16F0 plus b3-null;AktKO pericytes at 1:8 ratio (n = 10 per group). (D) Western blot analysis using mock- and Ik-Ba-SR-transfected WT or b3-null pericyte lysates. HSC70, loading control. (E) Protein-profiler cytokine arrays. Quantitation of fold difference in cytokine expression between mock- and Ik-Ba-SR-transfected b3-null pericytes (n = 4 dots from 2 independent experiments). (F) Conditioned media from mock- and Ik-Ba-SR-transfected b3-null pericytes were applied to B16F0, B16F10, and LLC cells and cell survival measured by MTS assay. Bar charts, means ± SEM relative to tumor cells treated with conditioned media from mock-transfected b3-null pericytes (n = 3 experimental repeats).
Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/addgene plasmid/product/Addgene inc
Average 93 stars, based on 1 article reviews
addgene plasmid - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier
prc49  (Addgene inc)
90
Addgene inc prc49
Figure 6. Deletion of Pericyte-b3-Integrin Enhances Tumor Cell Growth via Pericyte-pAkt-p65-Regulated Cytokine Production (A) Western blot analysis of b3-null pericytes with CRISPR-Cas9 deletion <t>of</t> <t>Akt1</t> (b3-null;AktKO PC) or <t>HGFR</t> (b3-null;HGFRKO PC) compared with b3-null;Cas9 controls. Hsc70, loading control. Blots representative of 3 separate experiments. Bar charts, average densitometric analysis relative to loading controls (n = 3 experimental repeats). (B) B16F0 cell survival after exposure to conditioned media from b3-null;AktKO, b3-null;HGFRKO, or b3-null;Cas9 pericytes (n = 16 technical replicates for 2 separate experiments). (C) Tumor growth in WT mice injected subcutaneously with either B16F0 cells plus b3-null;Cas9 pericytes or B16F0 plus b3-null;AktKO pericytes at 1:8 ratio (n = 10 per group). (D) Western blot analysis using mock- and Ik-Ba-SR-transfected WT or b3-null pericyte lysates. HSC70, loading control. (E) Protein-profiler cytokine arrays. Quantitation of fold difference in cytokine expression between mock- and Ik-Ba-SR-transfected b3-null pericytes (n = 4 dots from 2 independent experiments). (F) Conditioned media from mock- and Ik-Ba-SR-transfected b3-null pericytes were applied to B16F0, B16F10, and LLC cells and cell survival measured by MTS assay. Bar charts, means ± SEM relative to tumor cells treated with conditioned media from mock-transfected b3-null pericytes (n = 3 experimental repeats).
Prc49, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prc49/product/Addgene inc
Average 90 stars, based on 1 article reviews
prc49 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
plenti x2 blast pter shluc w618 1  (Addgene inc)
93
Addgene inc plenti x2 blast pter shluc w618 1
Figure 6. Deletion of Pericyte-b3-Integrin Enhances Tumor Cell Growth via Pericyte-pAkt-p65-Regulated Cytokine Production (A) Western blot analysis of b3-null pericytes with CRISPR-Cas9 deletion <t>of</t> <t>Akt1</t> (b3-null;AktKO PC) or <t>HGFR</t> (b3-null;HGFRKO PC) compared with b3-null;Cas9 controls. Hsc70, loading control. Blots representative of 3 separate experiments. Bar charts, average densitometric analysis relative to loading controls (n = 3 experimental repeats). (B) B16F0 cell survival after exposure to conditioned media from b3-null;AktKO, b3-null;HGFRKO, or b3-null;Cas9 pericytes (n = 16 technical replicates for 2 separate experiments). (C) Tumor growth in WT mice injected subcutaneously with either B16F0 cells plus b3-null;Cas9 pericytes or B16F0 plus b3-null;AktKO pericytes at 1:8 ratio (n = 10 per group). (D) Western blot analysis using mock- and Ik-Ba-SR-transfected WT or b3-null pericyte lysates. HSC70, loading control. (E) Protein-profiler cytokine arrays. Quantitation of fold difference in cytokine expression between mock- and Ik-Ba-SR-transfected b3-null pericytes (n = 4 dots from 2 independent experiments). (F) Conditioned media from mock- and Ik-Ba-SR-transfected b3-null pericytes were applied to B16F0, B16F10, and LLC cells and cell survival measured by MTS assay. Bar charts, means ± SEM relative to tumor cells treated with conditioned media from mock-transfected b3-null pericytes (n = 3 experimental repeats).
Plenti X2 Blast Pter Shluc W618 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plenti x2 blast pter shluc w618 1/product/Addgene inc
Average 93 stars, based on 1 article reviews
plenti x2 blast pter shluc w618 1 - by Bioz Stars, 2026-05
93/100 stars
  Buy from Supplier

Image Search Results


Figure 6. Deletion of Pericyte-b3-Integrin Enhances Tumor Cell Growth via Pericyte-pAkt-p65-Regulated Cytokine Production (A) Western blot analysis of b3-null pericytes with CRISPR-Cas9 deletion of Akt1 (b3-null;AktKO PC) or HGFR (b3-null;HGFRKO PC) compared with b3-null;Cas9 controls. Hsc70, loading control. Blots representative of 3 separate experiments. Bar charts, average densitometric analysis relative to loading controls (n = 3 experimental repeats). (B) B16F0 cell survival after exposure to conditioned media from b3-null;AktKO, b3-null;HGFRKO, or b3-null;Cas9 pericytes (n = 16 technical replicates for 2 separate experiments). (C) Tumor growth in WT mice injected subcutaneously with either B16F0 cells plus b3-null;Cas9 pericytes or B16F0 plus b3-null;AktKO pericytes at 1:8 ratio (n = 10 per group). (D) Western blot analysis using mock- and Ik-Ba-SR-transfected WT or b3-null pericyte lysates. HSC70, loading control. (E) Protein-profiler cytokine arrays. Quantitation of fold difference in cytokine expression between mock- and Ik-Ba-SR-transfected b3-null pericytes (n = 4 dots from 2 independent experiments). (F) Conditioned media from mock- and Ik-Ba-SR-transfected b3-null pericytes were applied to B16F0, B16F10, and LLC cells and cell survival measured by MTS assay. Bar charts, means ± SEM relative to tumor cells treated with conditioned media from mock-transfected b3-null pericytes (n = 3 experimental repeats).

Journal: Cell

Article Title: Cancer Burden Is Controlled by Mural Cell-β3-Integrin Regulated Crosstalk with Tumor Cells.

doi: 10.1016/j.cell.2020.02.003

Figure Lengend Snippet: Figure 6. Deletion of Pericyte-b3-Integrin Enhances Tumor Cell Growth via Pericyte-pAkt-p65-Regulated Cytokine Production (A) Western blot analysis of b3-null pericytes with CRISPR-Cas9 deletion of Akt1 (b3-null;AktKO PC) or HGFR (b3-null;HGFRKO PC) compared with b3-null;Cas9 controls. Hsc70, loading control. Blots representative of 3 separate experiments. Bar charts, average densitometric analysis relative to loading controls (n = 3 experimental repeats). (B) B16F0 cell survival after exposure to conditioned media from b3-null;AktKO, b3-null;HGFRKO, or b3-null;Cas9 pericytes (n = 16 technical replicates for 2 separate experiments). (C) Tumor growth in WT mice injected subcutaneously with either B16F0 cells plus b3-null;Cas9 pericytes or B16F0 plus b3-null;AktKO pericytes at 1:8 ratio (n = 10 per group). (D) Western blot analysis using mock- and Ik-Ba-SR-transfected WT or b3-null pericyte lysates. HSC70, loading control. (E) Protein-profiler cytokine arrays. Quantitation of fold difference in cytokine expression between mock- and Ik-Ba-SR-transfected b3-null pericytes (n = 4 dots from 2 independent experiments). (F) Conditioned media from mock- and Ik-Ba-SR-transfected b3-null pericytes were applied to B16F0, B16F10, and LLC cells and cell survival measured by MTS assay. Bar charts, means ± SEM relative to tumor cells treated with conditioned media from mock-transfected b3-null pericytes (n = 3 experimental repeats).

Article Snippet: SgRNAs were designed using the CRISPOR algorithm (http://crispor.tefor.net). sgRNA sequences targeting TIMP-1 (Gene ID: 21857), CXCL1 (Gene ID: 14825), CCL2 (Gene ID: 20296), Akt1 (Gene ID: 11651), and HGFR (Met) (Gene ID: 17295) (see Table S1) were cloned into the pLenti-CRISPR–EGFP plasmid (Addgene-#75159) using BsmBI enzyme site.

Techniques: Western Blot, CRISPR, Control, Injection, Transfection, Quantitation Assay, Expressing, MTS Assay