Journal: Viruses

Article Title: Assembly and Capsid Expansion Mechanism of Bacteriophage P22 Revealed by High-Resolution Cryo-EM Structures.

doi: 10.3390/v15020355

Figure Lengend Snippet: Figure 1. The cryo-EM structure of the P22 procapsid. (A) The overall structure of the procapsid. The seven subunits of the procapsid asymmetric unit are in different colors. (B) The segmented asymmetric unit from the local reconstruction of procapsid. (C) Accurate atomic models based on the density map in panel B. (D) Density maps of gp5 at 2.6 Å resolution superimposed on their atomic models. The gp5 structure is divided into six domains, namely, N-arm, P-domain, I-domain, A-domain, F-loop, and E-loop.

Article Snippet: The P22 phage (ATCC-97541-B1), which was purchased from the American Type Culture Collection (Manassas, VA, USA), was propagated on S. typhimurium for 4 h at 37 ◦C.

Techniques: Cryo-EM Sample Prep

Journal: Scientific Reports

Article Title: High mannose-specific lectin Msl mediates key interactions of the vaginal Lactobacillus plantarum isolate CMPG5300

doi: 10.1038/srep37339

Figure Lengend Snippet: Strains and plasmids used in this study.

Article Snippet: WCFS1 , Sequenced wild-type strain; single colony isolate of NCIMB 8826 from human saliva , .

Techniques: Isolation, Mutagenesis, Expressing, Bacteria

Journal: Bioengineered

Article Title: Engineering Saccharomyces cerevisiae for improvement in ethanol tolerance by accumulation of trehalose

doi: 10.1080/21655979.2016.1207019

Figure Lengend Snippet: Strains used in this study.

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from Bioresource Collection and Research Center, Food Industry Research and Development Institution, Hsinchu, Taiwan.

Techniques: Over Expression, Plasmid Preparation

Journal: Bioengineered

Article Title: Engineering Saccharomyces cerevisiae for improvement in ethanol tolerance by accumulation of trehalose

doi: 10.1080/21655979.2016.1207019

Figure Lengend Snippet: Construction of deletion cassette with long flanking homology regions by overlapping PCR. In the first round of PCR, the upstream sequence of the nth1 gene, the kanr marker gene and the downstream sequence of the nth1 gene were amplified in 3 different PCR reaction tubes. S. cerevisiae genomic DNA was used as template for amplification of 0.26 kb 5′ UP nth1 region and 0.4 kb 3′ DOWN nth1 region by using primers L1, L2, and L3, L4, respectively. The pFA6a-kanMX6 vector was used as a template for amplification of kanMX region by using primers kanMX-F and kanMX-R. In the second round of PCR, the 5′ UP nth1 region and kanMX region were fused together by using primers L1 and kanMX-R. In the third round of PCR, the 3′ DOWN nth1 region and second round PCR product (5′ UP nth1 region-kanMX region) were fused together by using primers L1 and L4. The third round PCR product (5′ UP nth1 region- kanMX region - 3′ DOWN nth1 region) was cloned into pGEMT vector for construction of the disruption cassette. After transformation of the disruption cassette into S. cerevisiae, homologous recombination takes place with the deletion of target gene.

Article Snippet: S. cerevisiae (BCRC 21685) was purchased from Bioresource Collection and Research Center, Food Industry Research and Development Institution, Hsinchu, Taiwan.

Techniques: Sequencing, Marker, Amplification, Plasmid Preparation, Clone Assay, Transformation Assay, Homologous Recombination

Journal: Frontiers in Microbiology

Article Title: Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211

doi: 10.3389/fmicb.2021.736583

Figure Lengend Snippet: Forces in Zymomonas mobilis flocculation. (A) Flocculent microscopic image of recombinant strains. (B) Flocculation rate. (C) Flocculation phenotype.

Article Snippet: The wild-type strain Z. mobilis CP4 was purchased from the China Center for Type Culture Collection (CCTTCC; Accession NO.CICC10232).

Techniques: Flocculation, Recombinant

Journal: Frontiers in Microbiology

Article Title: Functional Gene Identification and Corresponding Tolerant Mechanism of High Furfural-Tolerant Zymomonas mobilis Strain F211

doi: 10.3389/fmicb.2021.736583

Figure Lengend Snippet: Potential furfural tolerance mechanism of Z. mobilis .

Article Snippet: The wild-type strain Z. mobilis CP4 was purchased from the China Center for Type Culture Collection (CCTTCC; Accession NO.CICC10232).

Techniques:

Journal: Molecular Biology of the Cell

Article Title: A yeast FRET biosensor enlightens cAMP signaling

doi: 10.1091/mbc.E20-05-0319

Figure Lengend Snippet: S. cerevisiae strains used in this paper.

Article Snippet: W303-1A WT , MATa, leu2-3/112, ura3-1, trp1-1, his3-11/15, ade2-1, can1-100 , J. Thevelein, Katholieke Universiteit Leuven, Belgium.

Techniques: