Journal: Veterinary Research

Article Title: The local immune response of mice after Helicobacter suis infection: strain differences and distinction with Helicobacter pylori

doi: 10.1186/1297-9716-43-75

Figure Lengend Snippet: Number of colonizing H. suis bacteria. Shown is the average number of H. suis bacteria/milligram tissue in the stomach of BALB/c and C57BL/6 mice 59 days after experimental infection.

Article Snippet: Seven-week-old, female specific-pathogen-free BALB/c and C57BL/6 mice, free of Helicobacter spp., were purchased from Harlan NL (Horst, The Netherlands).

Techniques: Bacteria, Infection

Journal: Veterinary Research

Article Title: The local immune response of mice after Helicobacter suis infection: strain differences and distinction with Helicobacter pylori

doi: 10.1186/1297-9716-43-75

Figure Lengend Snippet: General cytokine expression profile after experimental H. suis infection in BALB/c and C57BL/6 mice. Shown are the mean fold changes in mRNA expression of indicated cytokines in H. suis -infected BALB/c and C57BL/6 mice. The mean fold change in the relevant uninfected control groups is equal to 1. An * indicates a statistically significant difference compared to uninfected control mice ( p < 0.05).

Article Snippet: Seven-week-old, female specific-pathogen-free BALB/c and C57BL/6 mice, free of Helicobacter spp., were purchased from Harlan NL (Horst, The Netherlands).

Techniques: Expressing, Infection, Control

Journal: Veterinary Research

Article Title: The local immune response of mice after Helicobacter suis infection: strain differences and distinction with Helicobacter pylori

doi: 10.1186/1297-9716-43-75

Figure Lengend Snippet: Cytokine expression profiles for each group of animals infected with H. suis strains HS1-9 and H. pylori strains SS1 or pMSS1. Shown are the mean fold changes of mRNA expression in HS1-HS9- and SS1- or pMSS1-infected BALB/c and C57BL/6 mice for IL-1β ( A ), IL-4 ( B ), IL-6 ( C ), IL-10 ( D ) and IL-17 ( E ). The mean fold change in the relevant uninfected control groups is equal to 1. An * indicates a significant upregulation of mRNA expression compared to uninfected control mice ( p < 0.05). An ** indicates a significant upregulation of mRNA expression compared to uninfected control mice ( p < 0.0056, i.e. the new cut-off p value obtained after Bonferroni correction for multiple comparisons).

Article Snippet: Seven-week-old, female specific-pathogen-free BALB/c and C57BL/6 mice, free of Helicobacter spp., were purchased from Harlan NL (Horst, The Netherlands).

Techniques: Expressing, Infection, Control

Journal: Veterinary Research

Article Title: The local immune response of mice after Helicobacter suis infection: strain differences and distinction with Helicobacter pylori

doi: 10.1186/1297-9716-43-75

Figure Lengend Snippet: Correlation between cytokine expression and H. suis colonization. Shown are the correlation analyses between IL-6 ( A ), IL-17 ( B ), MIP-2 ( C ), IL-1β ( D ) and IL-10 ( E ) mRNA expression levels and the number of colonizing H. suis bacteria in stomachs of indicated mouse strains (BALB/c and C57BL/6). Correlation was measured by Spearman’s Rho (ρ).

Article Snippet: Seven-week-old, female specific-pathogen-free BALB/c and C57BL/6 mice, free of Helicobacter spp., were purchased from Harlan NL (Horst, The Netherlands).

Techniques: Expressing, Bacteria

Journal: Redox Biology

Article Title: A novel role of KEAP1/PGAM5 complex: ROS sensor for inducing mitophagy

doi: 10.1016/j.redox.2021.102186

Figure Lengend Snippet: PGAM5 interferes with PINK1 processing independently of its phosphatase activity. A. PGAM5 mutant and isoform lacking phosphatase activity induce Parkin translocation. PC6 cells were transfected with Parkin-EYFP and PGAM5 wt, PGAM5 F244D or short PGAM5 isoform (isoform 2, UniProt #Q96HS1-2). ****P < 0.0001, n = 6 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. B. PGAM5 mutant lacking PARL/OMA1 cleaving site does not induce Parkin translocation. PC6 cells were transfected with Parkin-EYFP and PGAM5 or PGAM5 S24F. ****P < 0.0001, n = 6 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. C. TOMM7 overexpression increases the fraction of full-length PGAM5 in HEK293 cells expressing PGAM5-flag and TOMM7-myc. D. TOMM7 overexpression protects partially against PGAM5 induced Parkin translocation. PC6 cells were transfected with Parkin-EYFP, PGAM5 wt or/and TOMM7. ****P < 0.0001, n = 9 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. E. OMA1 and PARL protect partially against PGAM5 induced Parkin translocation. PC6 cells were transfected with Parkin-EYFP, PGAM5 wt and PARL or OMA1. ****P < 0.0001, n = 6 dishes, 20 fields per dish, One-way ANOVA followed by Sidak's multiple comparisons test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: PARL-FLAG , Addgene , Cat# 13639.

Techniques: Activity Assay, Mutagenesis, Translocation Assay, Transfection, Over Expression, Expressing

Journal: Redox Biology

Article Title: A novel role of KEAP1/PGAM5 complex: ROS sensor for inducing mitophagy

doi: 10.1016/j.redox.2021.102186

Figure Lengend Snippet:

Article Snippet: PARL-FLAG , Addgene , Cat# 13639.

Techniques: Recombinant, Lysis, Extraction, Blocking Assay, Isolation, DC Protein Assay, Caspase-Glo Assay, shRNA, Generated, Plasmid Preparation, Synthesized, Variant Assay

Journal: iScience

Article Title: Friendly fungi: Tropical insect families form partnerships with intracellular fungi related to pathogens

doi: 10.1016/j.isci.2024.110674

Figure Lengend Snippet:

Article Snippet: Sordaria fimicola , Carolina Biological Supply , Cat# 156290.

Techniques: Sequencing, Recombinant, Saline, Plasmid Preparation, Wright Stain, Picogreen Assay, Negative Control, Software

Journal: Oncogene

Article Title: KIBRA exhibits MST-independent functional regulation of the Hippo signaling pathway in mammals.

doi: 10.1038/onc.2012.196

Figure Lengend Snippet: Figure 4. KIBRA expression antagonises YAP-induced transcriptional regulation. (a) mRNA expression of the YAP target genes BMP2, FGF1, RASSF4 and PDGFb was assessed by quantitative real-time PCR in MCF10A-YAP cells overexpressing KIBRA, Willin or Merlin. mRNA levels were compared with the empty vector control MCF10A-YAP cells. Willin and KIBRA overexpression increased BMP2 and RASSF4 mRNA levels and decreased FGF1 and PDGFb mRNA levels. Means were calculated from Ct values from two analyses conducted in triplicate. Error bars represent ±s.e. (n ¼ 6). MCF10A-YAP-vector vs MCF10A-YAP-KIBRA or MCF10A-YAP-Willin for all the analysed genes: *Po0.05, **Po0.01, ***Po0.001 Student’s t-test. MCF10A-YAP-vector vs MCF10A-YAP-Merlin: CTGF (P ¼ 0.129), FGF1 (P ¼ 0.005), BMP2 (P ¼ 0.585), RASSF4 (P ¼ 0.307), PDGFb (P ¼ 0.06), Student’s t-test. (b) Immunoblot analysis of KIBRA, Willin or Merlin overexpression in MCF10A-YAP cells, showing 2.7, 1.8 and 8.6-fold increases compared to empty vector control. b-actin was used as a loading control.

Article Snippet: All experiments were carried out at 80–90% confluency. pBABE Nf2 (Johnson et al.45) was obtained from Addgene (plasmid 14116).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Plasmid Preparation, Control, Over Expression, Western Blot