whole-cell lysates Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Abcam 293t whole cell lysate
    293t Whole Cell Lysate, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/293t whole cell lysate/product/Abcam
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    293t whole cell lysate - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    94
    Abcam whole cell lysate
    Neither low protein high carbohydrate diet ( LPHC ) nor high protein low carbohydrate ( HPLC ) diet altered neither skeletal muscle glycogen content nor cellular signaling. (A) Tibialis anterior ( TA ) muscle glycogen content. The color legend in panel A is shared with panel B. (B) TA muscle cellular signaling determined by western blotting. The signal intensities are presented relative to chow (dashed line). Representative blots and a coomassie loading control are shown to the left. For p70S6K T389 and GCN 2 T899, 25 μ g of total protein were loaded, and the positive controls were HEK 293 and 100 <t>nmol/L</t> Calyculin A‐treated <t>HeLa</t> cell lysates, respectively. Data are expressed as mean ± SEM . N = 7 for chow and n = 8 for LPHC /HPLC.
    Whole Cell Lysate, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whole cell lysate/product/Abcam
    Average 94 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    whole cell lysate - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    94
    Abcam hek293 whole cell lysate
    Neither low protein high carbohydrate diet ( LPHC ) nor high protein low carbohydrate ( HPLC ) diet altered neither skeletal muscle glycogen content nor cellular signaling. (A) Tibialis anterior ( TA ) muscle glycogen content. The color legend in panel A is shared with panel B. (B) TA muscle cellular signaling determined by western blotting. The signal intensities are presented relative to chow (dashed line). Representative blots and a coomassie loading control are shown to the left. For p70S6K T389 and GCN 2 T899, 25 μ g of total protein were loaded, and the positive controls were HEK 293 and 100 <t>nmol/L</t> Calyculin A‐treated <t>HeLa</t> cell lysates, respectively. Data are expressed as mean ± SEM . N = 7 for chow and n = 8 for LPHC /HPLC.
    Hek293 Whole Cell Lysate, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hek293 whole cell lysate/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hek293 whole cell lysate - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    90
    Abcam t47d whole cell lysate
    Neither low protein high carbohydrate diet ( LPHC ) nor high protein low carbohydrate ( HPLC ) diet altered neither skeletal muscle glycogen content nor cellular signaling. (A) Tibialis anterior ( TA ) muscle glycogen content. The color legend in panel A is shared with panel B. (B) TA muscle cellular signaling determined by western blotting. The signal intensities are presented relative to chow (dashed line). Representative blots and a coomassie loading control are shown to the left. For p70S6K T389 and GCN 2 T899, 25 μ g of total protein were loaded, and the positive controls were HEK 293 and 100 <t>nmol/L</t> Calyculin A‐treated <t>HeLa</t> cell lysates, respectively. Data are expressed as mean ± SEM . N = 7 for chow and n = 8 for LPHC /HPLC.
    T47d Whole Cell Lysate, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t47d whole cell lysate/product/Abcam
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    t47d whole cell lysate - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    92
    Abcam thp1 whole cell lysate ab7913
    Neither low protein high carbohydrate diet ( LPHC ) nor high protein low carbohydrate ( HPLC ) diet altered neither skeletal muscle glycogen content nor cellular signaling. (A) Tibialis anterior ( TA ) muscle glycogen content. The color legend in panel A is shared with panel B. (B) TA muscle cellular signaling determined by western blotting. The signal intensities are presented relative to chow (dashed line). Representative blots and a coomassie loading control are shown to the left. For p70S6K T389 and GCN 2 T899, 25 μ g of total protein were loaded, and the positive controls were HEK 293 and 100 <t>nmol/L</t> Calyculin A‐treated <t>HeLa</t> cell lysates, respectively. Data are expressed as mean ± SEM . N = 7 for chow and n = 8 for LPHC /HPLC.
    Thp1 Whole Cell Lysate Ab7913, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/thp1 whole cell lysate ab7913/product/Abcam
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    thp1 whole cell lysate ab7913 - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    86
    Abcam k562 whole cell lysate
    Bleomycin hydrolase is strongly expressed in AY-27 cells but the protease inhibitor E-64 does not sensitize AY-27 to bleomycin. (a) Western blots of BLMH expression in <t>K562</t> (positive control), A431, T24, and AY-27 cells. E-64 treated cells are included as control. PCNA expression level is used as loading control. The image is from one representative experiment out of three. (b) Bleomycin cytotoxicity at two concentrations (5 and 50 IU mL −1 ) with and without E-64 treatment (concentrations used are listed in Table 1 ). Cell survival fractions were determined 48 h after 4 h-treatment with the drug(s). The data are from one representative experiment out of three (mean of 24 wells ± SD).
    K562 Whole Cell Lysate, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k562 whole cell lysate/product/Abcam
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    k562 whole cell lysate - by Bioz Stars, 2020-05
    86/100 stars
      Buy from Supplier

    94
    Abcam jurkat whole cell lysate
    Bleomycin hydrolase is strongly expressed in AY-27 cells but the protease inhibitor E-64 does not sensitize AY-27 to bleomycin. (a) Western blots of BLMH expression in <t>K562</t> (positive control), A431, T24, and AY-27 cells. E-64 treated cells are included as control. PCNA expression level is used as loading control. The image is from one representative experiment out of three. (b) Bleomycin cytotoxicity at two concentrations (5 and 50 IU mL −1 ) with and without E-64 treatment (concentrations used are listed in Table 1 ). Cell survival fractions were determined 48 h after 4 h-treatment with the drug(s). The data are from one representative experiment out of three (mean of 24 wells ± SD).
    Jurkat Whole Cell Lysate, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jurkat whole cell lysate/product/Abcam
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    jurkat whole cell lysate - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    91
    Novus Biologicals pc 12 whole cell lysate
    Bleomycin hydrolase is strongly expressed in AY-27 cells but the protease inhibitor E-64 does not sensitize AY-27 to bleomycin. (a) Western blots of BLMH expression in <t>K562</t> (positive control), A431, T24, and AY-27 cells. E-64 treated cells are included as control. PCNA expression level is used as loading control. The image is from one representative experiment out of three. (b) Bleomycin cytotoxicity at two concentrations (5 and 50 IU mL −1 ) with and without E-64 treatment (concentrations used are listed in Table 1 ). Cell survival fractions were determined 48 h after 4 h-treatment with the drug(s). The data are from one representative experiment out of three (mean of 24 wells ± SD).
    Pc 12 Whole Cell Lysate, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pc 12 whole cell lysate/product/Novus Biologicals
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    pc 12 whole cell lysate - by Bioz Stars, 2020-05
    91/100 stars
      Buy from Supplier

    94
    Abcam hepg2 cell lysate
    Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. <t>HepG2</t> cell lysate is included as a positive control.
    Hepg2 Cell Lysate, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepg2 cell lysate/product/Abcam
    Average 94 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    hepg2 cell lysate - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    90
    BEI Resources whole cell lysates
    Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. <t>HepG2</t> cell lysate is included as a positive control.
    Whole Cell Lysates, supplied by BEI Resources, used in various techniques. Bioz Stars score: 90/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whole cell lysates/product/BEI Resources
    Average 90 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    whole cell lysates - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    95
    Beyotime whole cell lysates
    Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. <t>HepG2</t> cell lysate is included as a positive control.
    Whole Cell Lysates, supplied by Beyotime, used in various techniques. Bioz Stars score: 95/100, based on 462 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whole cell lysates/product/Beyotime
    Average 95 stars, based on 462 article reviews
    Price from $9.99 to $1999.99
    whole cell lysates - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    94
    Abcam wi 38 whole cell lysate
    Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. <t>HepG2</t> cell lysate is included as a positive control.
    Wi 38 Whole Cell Lysate, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wi 38 whole cell lysate/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    wi 38 whole cell lysate - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    92
    Interchim whole cell lysates
    Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. <t>HepG2</t> cell lysate is included as a positive control.
    Whole Cell Lysates, supplied by Interchim, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whole cell lysates/product/Interchim
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    whole cell lysates - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    93
    Abcam cell lysates
    Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. <t>HepG2</t> cell lysate is included as a positive control.
    Cell Lysates, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 3002 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell lysates/product/Abcam
    Average 93 stars, based on 3002 article reviews
    Price from $9.99 to $1999.99
    cell lysates - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    95
    Cell Signaling Technology Inc whole cell lysates
    Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. <t>HepG2</t> cell lysate is included as a positive control.
    Whole Cell Lysates, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1440 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whole cell lysates/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1440 article reviews
    Price from $9.99 to $1999.99
    whole cell lysates - by Bioz Stars, 2020-05
    95/100 stars
      Buy from Supplier

    94
    Thermo Fisher whole cell lysate
    Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. <t>HepG2</t> cell lysate is included as a positive control.
    Whole Cell Lysate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/whole cell lysate/product/Thermo Fisher
    Average 94 stars, based on 619 article reviews
    Price from $9.99 to $1999.99
    whole cell lysate - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    94
    Abcam caco 2 whole cell lysate
    Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. <t>HepG2</t> cell lysate is included as a positive control.
    Caco 2 Whole Cell Lysate, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caco 2 whole cell lysate/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    caco 2 whole cell lysate - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    88
    Abcam c2c12 cells
    C/EBPβ protein levels are regulated during myogenesis by the 26S proteasome. A , C/EBPβ LAP* and LAP protein expression in <t>C2C12</t> myoblasts in growth medium ( GM ) or after induction to differentiate in low serum differentiation medium ( DM
    C2c12 Cells, supplied by Abcam, used in various techniques. Bioz Stars score: 88/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c2c12 cells/product/Abcam
    Average 88 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    c2c12 cells - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    Image Search Results


    Neither low protein high carbohydrate diet ( LPHC ) nor high protein low carbohydrate ( HPLC ) diet altered neither skeletal muscle glycogen content nor cellular signaling. (A) Tibialis anterior ( TA ) muscle glycogen content. The color legend in panel A is shared with panel B. (B) TA muscle cellular signaling determined by western blotting. The signal intensities are presented relative to chow (dashed line). Representative blots and a coomassie loading control are shown to the left. For p70S6K T389 and GCN 2 T899, 25 μ g of total protein were loaded, and the positive controls were HEK 293 and 100 nmol/L Calyculin A‐treated HeLa cell lysates, respectively. Data are expressed as mean ± SEM . N = 7 for chow and n = 8 for LPHC /HPLC.

    Journal: Physiological Reports

    Article Title: Low‐ and high‐protein diets do not alter ex vivo insulin action in skeletal muscle. Low‐ and high‐protein diets do not alter ex vivo insulin action in skeletal muscle

    doi: 10.14814/phy2.13798

    Figure Lengend Snippet: Neither low protein high carbohydrate diet ( LPHC ) nor high protein low carbohydrate ( HPLC ) diet altered neither skeletal muscle glycogen content nor cellular signaling. (A) Tibialis anterior ( TA ) muscle glycogen content. The color legend in panel A is shared with panel B. (B) TA muscle cellular signaling determined by western blotting. The signal intensities are presented relative to chow (dashed line). Representative blots and a coomassie loading control are shown to the left. For p70S6K T389 and GCN 2 T899, 25 μ g of total protein were loaded, and the positive controls were HEK 293 and 100 nmol/L Calyculin A‐treated HeLa cell lysates, respectively. Data are expressed as mean ± SEM . N = 7 for chow and n = 8 for LPHC /HPLC.

    Article Snippet: For the cell culture positive control lysates, confluent 6‐well dishes with fed HEK293 cells were lysed in 100 μ L of lysis buffer, and HeLa cells lysates stimulated with 100 nmol/L calyculin A for 30 min was a gift from Abcam.

    Techniques: High Performance Liquid Chromatography, Western Blot

    Bleomycin hydrolase is strongly expressed in AY-27 cells but the protease inhibitor E-64 does not sensitize AY-27 to bleomycin. (a) Western blots of BLMH expression in K562 (positive control), A431, T24, and AY-27 cells. E-64 treated cells are included as control. PCNA expression level is used as loading control. The image is from one representative experiment out of three. (b) Bleomycin cytotoxicity at two concentrations (5 and 50 IU mL −1 ) with and without E-64 treatment (concentrations used are listed in Table 1 ). Cell survival fractions were determined 48 h after 4 h-treatment with the drug(s). The data are from one representative experiment out of three (mean of 24 wells ± SD).

    Journal: BioMed Research International

    Article Title: Enhanced Efficacy of Bleomycin in Bladder Cancer Cells by Photochemical Internalization

    doi: 10.1155/2014/921296

    Figure Lengend Snippet: Bleomycin hydrolase is strongly expressed in AY-27 cells but the protease inhibitor E-64 does not sensitize AY-27 to bleomycin. (a) Western blots of BLMH expression in K562 (positive control), A431, T24, and AY-27 cells. E-64 treated cells are included as control. PCNA expression level is used as loading control. The image is from one representative experiment out of three. (b) Bleomycin cytotoxicity at two concentrations (5 and 50 IU mL −1 ) with and without E-64 treatment (concentrations used are listed in Table 1 ). Cell survival fractions were determined 48 h after 4 h-treatment with the drug(s). The data are from one representative experiment out of three (mean of 24 wells ± SD).

    Article Snippet: K562 whole cell lysate (30 μ g, ab7911, Abcam) was used as positive control of the primary antibody.

    Techniques: Protease Inhibitor, Western Blot, Expressing, Positive Control

    Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. HepG2 cell lysate is included as a positive control.

    Journal: F1000Research

    Article Title: Functional proteomic profiling reveals KLK13 and TMPRSS11D as active proteases in the lower female reproductive tract

    doi: 10.12688/f1000research.16255.2

    Figure Lengend Snippet: Validation of TMPRSS1D in cervical-vaginal fluid (CVF). TMPRSS11D is detected in unfractionated CVF and in F42-F46 of SEC fractionated CVF corresponding with the proteolytic activity visualized with the ABP in the low MW band. HepG2 cell lysate is included as a positive control.

    Article Snippet: A total of 20µg HepG2 cell lysate (abcam, #ab7900) was included as a positive control as per manufacturer’s instructions.

    Techniques: Size-exclusion Chromatography, Activity Assay, Positive Control

    C/EBPβ protein levels are regulated during myogenesis by the 26S proteasome. A , C/EBPβ LAP* and LAP protein expression in C2C12 myoblasts in growth medium ( GM ) or after induction to differentiate in low serum differentiation medium ( DM

    Journal: The Journal of Biological Chemistry

    Article Title: Mdm2 Promotes Myogenesis through the Ubiquitination and Degradation of CCAAT/Enhancer-binding Protein β

    doi: 10.1074/jbc.M115.638577

    Figure Lengend Snippet: C/EBPβ protein levels are regulated during myogenesis by the 26S proteasome. A , C/EBPβ LAP* and LAP protein expression in C2C12 myoblasts in growth medium ( GM ) or after induction to differentiate in low serum differentiation medium ( DM

    Article Snippet: Immunoprecipitation of proteins from whole cell extracts from C2C12 cells was performed using anti-C/EBPβ antibody E299 (Abcam) or anti-Mdm2 antibody C18 (Santa Cruz Biotechnology) and co-precipitated C/EBPβ or Mdm2 was detected by Western blotting using the same antibodies.

    Techniques: Expressing

    Mdm2 ubiquitinates and targets C/EBPβ for degradation. A , C/EBPβ and Mdm2 protein expression in C2C12 cells lentivirally transduced to express shRNA against Mdm2 ( shMdm2 ) or a scrambled shRNA ( shScr ) and induced to differentiate for 24

    Journal: The Journal of Biological Chemistry

    Article Title: Mdm2 Promotes Myogenesis through the Ubiquitination and Degradation of CCAAT/Enhancer-binding Protein β

    doi: 10.1074/jbc.M115.638577

    Figure Lengend Snippet: Mdm2 ubiquitinates and targets C/EBPβ for degradation. A , C/EBPβ and Mdm2 protein expression in C2C12 cells lentivirally transduced to express shRNA against Mdm2 ( shMdm2 ) or a scrambled shRNA ( shScr ) and induced to differentiate for 24

    Article Snippet: Immunoprecipitation of proteins from whole cell extracts from C2C12 cells was performed using anti-C/EBPβ antibody E299 (Abcam) or anti-Mdm2 antibody C18 (Santa Cruz Biotechnology) and co-precipitated C/EBPβ or Mdm2 was detected by Western blotting using the same antibodies.

    Techniques: Expressing, shRNA

    Loss of Mdm2 blocks myogenic differentiation and regeneration. A , immunocytochemistry for myosin heavy chain (MyHC) expression in C2C12 cells transduced to express shMdm2 or shScr and induced to differentiate for 7 days. DAPI stain reveals nuclei. B ,

    Journal: The Journal of Biological Chemistry

    Article Title: Mdm2 Promotes Myogenesis through the Ubiquitination and Degradation of CCAAT/Enhancer-binding Protein β

    doi: 10.1074/jbc.M115.638577

    Figure Lengend Snippet: Loss of Mdm2 blocks myogenic differentiation and regeneration. A , immunocytochemistry for myosin heavy chain (MyHC) expression in C2C12 cells transduced to express shMdm2 or shScr and induced to differentiate for 7 days. DAPI stain reveals nuclei. B ,

    Article Snippet: Immunoprecipitation of proteins from whole cell extracts from C2C12 cells was performed using anti-C/EBPβ antibody E299 (Abcam) or anti-Mdm2 antibody C18 (Santa Cruz Biotechnology) and co-precipitated C/EBPβ or Mdm2 was detected by Western blotting using the same antibodies.

    Techniques: Immunocytochemistry, Expressing, Staining

    Mdm2 expression is regulated during myogenesis. A , C/EBPβ, Mdm2, and myogenic marker protein expression in C2C12 myoblasts cultured in GM or induced in DM for 1–9 days. α-Tubulin is a loading control. B , Western analysis of p53

    Journal: The Journal of Biological Chemistry

    Article Title: Mdm2 Promotes Myogenesis through the Ubiquitination and Degradation of CCAAT/Enhancer-binding Protein β

    doi: 10.1074/jbc.M115.638577

    Figure Lengend Snippet: Mdm2 expression is regulated during myogenesis. A , C/EBPβ, Mdm2, and myogenic marker protein expression in C2C12 myoblasts cultured in GM or induced in DM for 1–9 days. α-Tubulin is a loading control. B , Western analysis of p53

    Article Snippet: Immunoprecipitation of proteins from whole cell extracts from C2C12 cells was performed using anti-C/EBPβ antibody E299 (Abcam) or anti-Mdm2 antibody C18 (Santa Cruz Biotechnology) and co-precipitated C/EBPβ or Mdm2 was detected by Western blotting using the same antibodies.

    Techniques: Expressing, Marker, Cell Culture, Western Blot