way213613 Search Results


ifs way213613  (Tocris)
93
Tocris ifs way213613
Ifs Way213613, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ifs way213613 - by Bioz Stars, 2026-02
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way 213613  (MedChemExpress)
93
MedChemExpress way 213613
Way 213613, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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way 213613 - by Bioz Stars, 2026-02
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way213613  (Tocris)
94
Tocris way213613
PS1 alters GLT-1 mediated glutamate uptake in HEK cells after 10 min glutamate treatment. a [ 3 H]-L-glutamate uptake was assayed in HEK PS DKO cells stably expressing GLT-1. Cells were transfected with PS1 or control vector (see methods). Two days after transfection, experiment was performed in which cells were exposed to [. 3 H]-L-glutamate in a total glutamate concentration of 50 μM for 5 and 10 min at 37 °C. There were four groups of cells, two in which the GLT-1 specific inhibitors DHK (1 mM) or <t>WAY213613</t> (10 uM) were present, and two others in which vehicle only was present. Four separate experiments were performed, each representing a separate passage and plating of the HEK cells, and the results shown are data pooled from those 4 experiments. Results are all normalized to the control (vehicle only) value from the PS1 negative (pc DNA-GFP) condition at 5 min. Error bars show SEM, **** p < 0.0001. Two-Way ANOVA mixed-effects analysis with Sidak’s multiple comparisons test b Western blot of cell lysate used for GLT-1 glutamate uptake. Cell cultures for Western blotting were not exposed to [3H]-L-glutamate but were treated with sodium only, and the assay was performed in parallel with the glutamate uptake experiments. Membrane was probe with GLT-1 (ABCAM) antibody and PS1 antibody (CST)
Way213613, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
way213613 - by Bioz Stars, 2026-02
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glt1 mediated glutamate transport  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology glt1 mediated glutamate transport
PS1 alters GLT-1 mediated glutamate uptake in HEK cells after 10 min glutamate treatment. a [ 3 H]-L-glutamate uptake was assayed in HEK PS DKO cells stably expressing GLT-1. Cells were transfected with PS1 or control vector (see methods). Two days after transfection, experiment was performed in which cells were exposed to [. 3 H]-L-glutamate in a total glutamate concentration of 50 μM for 5 and 10 min at 37 °C. There were four groups of cells, two in which the GLT-1 specific inhibitors DHK (1 mM) or <t>WAY213613</t> (10 uM) were present, and two others in which vehicle only was present. Four separate experiments were performed, each representing a separate passage and plating of the HEK cells, and the results shown are data pooled from those 4 experiments. Results are all normalized to the control (vehicle only) value from the PS1 negative (pc DNA-GFP) condition at 5 min. Error bars show SEM, **** p < 0.0001. Two-Way ANOVA mixed-effects analysis with Sidak’s multiple comparisons test b Western blot of cell lysate used for GLT-1 glutamate uptake. Cell cultures for Western blotting were not exposed to [3H]-L-glutamate but were treated with sodium only, and the assay was performed in parallel with the glutamate uptake experiments. Membrane was probe with GLT-1 (ABCAM) antibody and PS1 antibody (CST)
Glt1 Mediated Glutamate Transport, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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way-213613  (Wyeth Biopharma)
90
Wyeth Biopharma way-213613
PS1 alters GLT-1 mediated glutamate uptake in HEK cells after 10 min glutamate treatment. a [ 3 H]-L-glutamate uptake was assayed in HEK PS DKO cells stably expressing GLT-1. Cells were transfected with PS1 or control vector (see methods). Two days after transfection, experiment was performed in which cells were exposed to [. 3 H]-L-glutamate in a total glutamate concentration of 50 μM for 5 and 10 min at 37 °C. There were four groups of cells, two in which the GLT-1 specific inhibitors DHK (1 mM) or <t>WAY213613</t> (10 uM) were present, and two others in which vehicle only was present. Four separate experiments were performed, each representing a separate passage and plating of the HEK cells, and the results shown are data pooled from those 4 experiments. Results are all normalized to the control (vehicle only) value from the PS1 negative (pc DNA-GFP) condition at 5 min. Error bars show SEM, **** p < 0.0001. Two-Way ANOVA mixed-effects analysis with Sidak’s multiple comparisons test b Western blot of cell lysate used for GLT-1 glutamate uptake. Cell cultures for Western blotting were not exposed to [3H]-L-glutamate but were treated with sodium only, and the assay was performed in parallel with the glutamate uptake experiments. Membrane was probe with GLT-1 (ABCAM) antibody and PS1 antibody (CST)
Way 213613, supplied by Wyeth Biopharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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way-213613 hydrochloride  (MedChemExpress)
90
MedChemExpress way-213613 hydrochloride
PS1 alters GLT-1 mediated glutamate uptake in HEK cells after 10 min glutamate treatment. a [ 3 H]-L-glutamate uptake was assayed in HEK PS DKO cells stably expressing GLT-1. Cells were transfected with PS1 or control vector (see methods). Two days after transfection, experiment was performed in which cells were exposed to [. 3 H]-L-glutamate in a total glutamate concentration of 50 μM for 5 and 10 min at 37 °C. There were four groups of cells, two in which the GLT-1 specific inhibitors DHK (1 mM) or <t>WAY213613</t> (10 uM) were present, and two others in which vehicle only was present. Four separate experiments were performed, each representing a separate passage and plating of the HEK cells, and the results shown are data pooled from those 4 experiments. Results are all normalized to the control (vehicle only) value from the PS1 negative (pc DNA-GFP) condition at 5 min. Error bars show SEM, **** p < 0.0001. Two-Way ANOVA mixed-effects analysis with Sidak’s multiple comparisons test b Western blot of cell lysate used for GLT-1 glutamate uptake. Cell cultures for Western blotting were not exposed to [3H]-L-glutamate but were treated with sodium only, and the assay was performed in parallel with the glutamate uptake experiments. Membrane was probe with GLT-1 (ABCAM) antibody and PS1 antibody (CST)
Way 213613 Hydrochloride, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


PS1 alters GLT-1 mediated glutamate uptake in HEK cells after 10 min glutamate treatment. a [ 3 H]-L-glutamate uptake was assayed in HEK PS DKO cells stably expressing GLT-1. Cells were transfected with PS1 or control vector (see methods). Two days after transfection, experiment was performed in which cells were exposed to [. 3 H]-L-glutamate in a total glutamate concentration of 50 μM for 5 and 10 min at 37 °C. There were four groups of cells, two in which the GLT-1 specific inhibitors DHK (1 mM) or WAY213613 (10 uM) were present, and two others in which vehicle only was present. Four separate experiments were performed, each representing a separate passage and plating of the HEK cells, and the results shown are data pooled from those 4 experiments. Results are all normalized to the control (vehicle only) value from the PS1 negative (pc DNA-GFP) condition at 5 min. Error bars show SEM, **** p < 0.0001. Two-Way ANOVA mixed-effects analysis with Sidak’s multiple comparisons test b Western blot of cell lysate used for GLT-1 glutamate uptake. Cell cultures for Western blotting were not exposed to [3H]-L-glutamate but were treated with sodium only, and the assay was performed in parallel with the glutamate uptake experiments. Membrane was probe with GLT-1 (ABCAM) antibody and PS1 antibody (CST)

Journal: Acta Neuropathologica Communications

Article Title: PS1/gamma-secretase acts as rogue chaperone of glutamate transporter EAAT2/GLT-1 in Alzheimer’s disease

doi: 10.1186/s40478-024-01876-y

Figure Lengend Snippet: PS1 alters GLT-1 mediated glutamate uptake in HEK cells after 10 min glutamate treatment. a [ 3 H]-L-glutamate uptake was assayed in HEK PS DKO cells stably expressing GLT-1. Cells were transfected with PS1 or control vector (see methods). Two days after transfection, experiment was performed in which cells were exposed to [. 3 H]-L-glutamate in a total glutamate concentration of 50 μM for 5 and 10 min at 37 °C. There were four groups of cells, two in which the GLT-1 specific inhibitors DHK (1 mM) or WAY213613 (10 uM) were present, and two others in which vehicle only was present. Four separate experiments were performed, each representing a separate passage and plating of the HEK cells, and the results shown are data pooled from those 4 experiments. Results are all normalized to the control (vehicle only) value from the PS1 negative (pc DNA-GFP) condition at 5 min. Error bars show SEM, **** p < 0.0001. Two-Way ANOVA mixed-effects analysis with Sidak’s multiple comparisons test b Western blot of cell lysate used for GLT-1 glutamate uptake. Cell cultures for Western blotting were not exposed to [3H]-L-glutamate but were treated with sodium only, and the assay was performed in parallel with the glutamate uptake experiments. Membrane was probe with GLT-1 (ABCAM) antibody and PS1 antibody (CST)

Article Snippet: In the experiments using γ-secretase modulators (GSMs), cells were treated with 20 nM GSM15606 [ ], 3 μM GSM36 [ ] or dimethyl sulfoxide (DMSO) vehicle control for 16 h. GLT-1 selective inhibitors, DHK (dihydrokainate) and WAY213613 were obtained from Tocris.

Techniques: Stable Transfection, Expressing, Transfection, Control, Plasmid Preparation, Concentration Assay, Western Blot, Membrane