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Image Search Results
Journal: Frontiers in Immunology
Article Title: Canagliflozin reverses Th1/Th2 imbalance and promotes podocyte autophagy in rats with membranous nephropathy
doi: 10.3389/fimmu.2022.993869
Figure Lengend Snippet: Canagliflozin regulates the imbalance in Th1/Th2 in MN rats. (A) Representative images showing DAPI (blue) and SGLT2 (green) staining in vitro T lymphocytes. (B–D) The number of CD4+ T-cells and CD8+ T-cells in peripheral blood PBMCs of rats detected by flow cytometry analysis. (E–H) The number of Th1 cells and Th2 cells in peripheral blood PBMCs of rats detected by flow cytometry analysis. Data are shown as mean ± SEM values. One-way ANOVA followed by Tukey’s test for multiple comparisons was used. *P < 0.05, **P < 0.01.
Article Snippet: Flow cytometry was used to determine the phenotype of each group of rat T-cells, B-cells, and Th1 and Th2 cells using various combinations of the following fluorochrome-conjugated monoclonal antibodies: perCP/Cyanine5.5-conjugated anti-rat CD3 (201417; Biolegend, USA), FITC-conjugated
Techniques: Staining, In Vitro, Flow Cytometry