Journal: PLoS ONE

Article Title: In Vivo Serial MR Imaging of Magnetically Labeled Endothelial Progenitor Cells Homing to the Endothelium Injured Artery in Mice

doi: 10.1371/journal.pone.0020790

Figure Lengend Snippet: 7 days after in vitro differentiation, spleen-derived EPCs showed expression of CD31 (B), CD34 (C) and vWF (D) with the cytoplasm stained in brown. Photo A is the control. Hematoxylin-stained nuclei were shown in blue. (Magnification ×400).

Article Snippet: After 7 days in culture, spleen-derived EPCs in 4 wells of a 24-well culture plate were extensively washed, and fixed in ice-cold 4% paraformaldehyde for 20 min. Immunocytochemistry was performed using antibodies against CD31, CD34 (Wuhan Boster Biological Technology, LTD. Wuhan, China) and von Willebrand factor (vWF) ( Zhongshan Goldenbridge Biotechnology Co., LTD. Beijing, China) according to standard protocols.

Techniques: In Vitro, Derivative Assay, Expressing, Staining

Journal: Experimental and Therapeutic Medicine

Article Title: Construction of tissue-engineered bone using a bioreactor and platelet-rich plasma

doi: 10.3892/etm.2014.1774

Figure Lengend Snippet: Immunohistochemical analysis of vWF expression. Each rabbit was implanted at five different sites with β-TCP only (group A), BMSCs and β-TCP (group B), bioreactor-cultured BMSCs and β-TCP (group C), PRP-cultured BMSCs and β-TCP (group D), and bioreactor- and PRP-cultured BMSCs and β-TCP (group E), respectively. Three months following implantation, samples were collected from implanted rabbits (n=10). vWF expression was detected using immunohistochemistry. Cells with brown granules were considered positive cells. Expression of vWF was analyzed by immunohistochemistry. Representative immunohistochemical results from (A) group A, (B) group B, (C) group C, (D) group D and (E) group E (magnification, ×200). vWF, von Willebrand Factor; TCP, tricalcium phosphate; BMSCs, bone marrow mesenchymal stem cells; PRP, platelet-rich plasma.

Article Snippet: Hematoxylin and eosin (H&E), anti-cluster of differentiation (CD)31 antibody, anti-von Willebrand factor (vWF) antibody and 3,3′-diaminobenzidine (DAB) chromogenic reagent for immunohistochemistry were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Immunohistochemical staining, Expressing, Cell Culture, Immunohistochemistry, Clinical Proteomics

Journal: Frontiers in Cardiovascular Medicine

Article Title: Pro-inflammatory role of Wnt/β-catenin signaling in endothelial dysfunction

doi: 10.3389/fcvm.2022.1059124

Figure Lengend Snippet: Schematic diagram illustrating effect of inhibition of Wnt/β-catenin signaling on endothelial-platelet interaction in unchallenged and TNF-α-challenged cultured endothelial cells. Endothelial cells were treated with either 0.05% DMSO vehicle control or 25 μM iCRT in the presence or absence of 10 ng/mL recombinant human TNF-α stimulus for 18 h, then co-cultured with thrombin-activated platelets for 10 min. (A) Control: Unchallenged endothelial cells treated with 0.05% DMSO vehicle control. UL-vWF multimers remain stored in the Weibel-Palade bodies. (B) Control + iCRT-14: Unchallenged endothelial cells treated with 25 μM iCRT-14. UL-vWF multimers remain stored in the Weibel-Palade bodies. (C) TNF-α: TNF-α-challenged endothelial cells treated with 0.05% DMSO vehicle control. TNF-α stimulates release of UL-vWF from Weibel–Palade bodies. TNF-α-driven up-regulation in ADAMTS13 results in proteolytic cleavage of membrane-tethered UL-vWF; hence, TNF-α-stimulation does not promote endothelial-platelet interaction. (D) TNF-α + iCRT-14: TNF-α-challenged endothelial cells treated with 25 μM iCRT-14. TNF-α stimulates release of UL-vWF from Weibel–Palade bodies. Treatment with iCRT-14 blocks TNF-a-mediated up-regulation of ADAMTS13 thereby maintaining high levels of membrane-tethered UL-vWF; hence, platelet recruitment is enhanced. Acronyms: ADAMTS13 - a disintegrin-like and metalloprotease with thrombospondin type-1 repeats-13; TNF-α - tumor necrosis factor-a; UL-vWF - ultra-large von Willebrand factor.

Article Snippet: Primary antibodies included: anti-cleaved caspase-3 IgG (R&D Systems; MAB835), anti-NFκB p65 IgG (Cell Signaling Technology; 8242), anti-phospho-paxillin (Tyr118) IgG (Invitrogen; 44-722G), anti-VE-Cadherin IgG (Cell Signaling Technology; 2500), anti-von Willebrand factor IgG (Cell Signaling; 65707S), and anti-ZO-1 IgG (Abcam; ab221547).

Techniques: Inhibition, Cell Culture, Control, Recombinant, Membrane

Journal: ACS Measurement Science Au

Article Title: Electrochemical Profiling of vWFA2 for Systemic Inflammatory State Detection

doi: 10.1021/acsmeasuresciau.4c00060

Figure Lengend Snippet: Schematic representation of the working mechanism of the developed biosensor as a point-of-care diagnostic device for the quantification of vWFA2 levels.

Article Snippet: A specific recombinant human vWFA2 antibody and protein were purchased from R&D Systems.

Techniques: Diagnostic Assay

Journal: ACS Measurement Science Au

Article Title: Electrochemical Profiling of vWFA2 for Systemic Inflammatory State Detection

doi: 10.1021/acsmeasuresciau.4c00060

Figure Lengend Snippet: Bode (magnitude) (a), Bode (phase) (b), and Nyquist (c) plots representing impedance measurements at each concentration of vWFA2 in pooled plasma samples.

Article Snippet: A specific recombinant human vWFA2 antibody and protein were purchased from R&D Systems.

Techniques: Concentration Assay

Journal: ACS Measurement Science Au

Article Title: Electrochemical Profiling of vWFA2 for Systemic Inflammatory State Detection

doi: 10.1021/acsmeasuresciau.4c00060

Figure Lengend Snippet: Biosensor response against cross-reactive molecule (IP-10) in the presence of BSA and vWFA2 at low (a) and high (b) concentrations.

Article Snippet: A specific recombinant human vWFA2 antibody and protein were purchased from R&D Systems.

Techniques:

Journal: ACS Measurement Science Au

Article Title: Electrochemical Profiling of vWFA2 for Systemic Inflammatory State Detection

doi: 10.1021/acsmeasuresciau.4c00060

Figure Lengend Snippet: (a, b) Intra-assay variability (left y -axis) and Interassay variability (right y -axis) of the developed biosensor for various concentrations of vWFA2.

Article Snippet: A specific recombinant human vWFA2 antibody and protein were purchased from R&D Systems.

Techniques: Intra Assay