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Journal: bioRxiv
Article Title: Site-specific epigenetic marks in Trypanosoma brucei transcription termination, antigenic variation, and proliferation
doi: 10.1101/2021.05.13.444086
Figure Lengend Snippet: VSG RNA levels in WT and KO mutants were analyzed by rRNA-depleted stranded RNA-seq. RNA-seq reads were aligned to the VSGnome database and LOG2(RPKM) values were obtained for each VSG in wild type and KO mutants. (A) Four types of VSG loci in the genome. BES VSG (telomeric, polycistronically transcribed by RNA pol I), MES VSG (telomeric, monicistronically transcribed by RNA pol I), and MC VSG (telomeric, no promoter). Chromosome-internal VSG in arrays. (B) Heat map comparison between WT and KO mutants for BES and MES VSG expression levels (darker color = higher expression levels). Shown: silent VSGs (red) and active VSG2 (green). (C) Bar graphs comparing WT and KO mutants for BES and MES VSG expression levels. Mutants are grouped by the loss of VSG silencing phenotype: mutants that showed no change (top) and mutants that increased the expression of silent VSGs (bottom). Error bars indicate standard deviation between three replicates. (D) Box plot comparing WT and KO mutants for MC VSG expression levels. (E) Box plot comparing WT and KO mutants for chromosome internal VSG expression levels. Statistically analyses are summarized in Supplementary Table 9.
Article Snippet: 6 million live cells were stained with
Techniques: RNA Sequencing Assay, Expressing, Standard Deviation