Journal: Nature Communications

Article Title: Non-canonical Wnt signaling promotes epithelial fluidization in the repairing airway

doi: 10.1038/s41467-025-59320-1

Figure Lengend Snippet: a Staining and quantification of YAP in tdTomato+ BCs reveals an increase of YAP activity after 1d post polidocanol injury. Ptk7 loss of function decreases YAP activation after injury. b Montage and quantification of BC migratory behavior in laser-ablated trachea after treatment with DMSO or YAP inhibitor. DMSO controls were taken from Fig. . Red line indicates diameter of the wound. mean ± SD; n = sample size as follows, a 3 mice (quantified cells pooled from two sections per mouse), b 5 (Mock) and 4 (Verteporfin) mice. ** P < 0.01 ( a Control Undamaged vs Control 1dp polidocanol = 0.0026, Control 1dp polidocanol vs Ptk7 KO 1dp polidocanol = 0.0049), *** P < 0.001 ( b < 0.0001), based on one-way ANOVA with Tukey’s multiple comparison test ( a ) and two-tailed, unpaired Student’s t -test ( b ). Scale bar = 10 µm. Timestamp indicated as hours:minutes, with 00:00 recorded immediately following ablation.

Article Snippet: Small molecule inhibitors and concentrations used in this study: Cytochalasin B (10 μg/ml, Sigma Aldrich, C6762), dFzd7-21 (100 μg/ml, Genentech, Inc.) , , LGK974 (20 μM, Sigma Aldrich Chemicals, 531091), iCrt14 (30 μM, Millipore, SML0203), Verteporfin (10 μM, Selleck Chemical, S178610), Wnt5a (500 ng/ml, Novus, 645-WN-010), and Wnt3a (500 ng/ml, Novus, 1324-WN-010).

Techniques: Staining, Activity Assay, Activation Assay, Control, Comparison, Two Tailed Test

Journal: Molecular Vision

Article Title: In vitro effects of verteporfin on ocular cells

doi:

Figure Lengend Snippet: Viability of cultured ocular cells exposed to a range of verteporfin for 24 h without exposure to laser light. The percent of live cells was determined with 3-(4,5- dimethyl-2-thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay in human scleral fibroblasts (hFibro), human trabecular meshwork cells (hTMC), and a human retinal pigment epithelial cell line (ARPE-19). Data were normalized to untreated cells (0 µg/ml verteporfin; 100% Live), and plotted as the mean (n=4) with error bars representing the standard deviation. *=p<0.05 versus 0 µg/ml verteporfin treatment.

Article Snippet: Verteporfin (Visudyne, QLT Ophthalmics Inc., Menlo Park, CA) came as a lyophilized powder of 15 mg active ingredient in approximately 765 mg of inactive ingredients.

Techniques: Cell Culture, MTT Assay, Standard Deviation

Journal: Molecular Vision

Article Title: In vitro effects of verteporfin on ocular cells

doi:

Figure Lengend Snippet: Viability of cultured ocular cells exposed to 0.5 µg/ml verteporfin with different intensities of laser light. The percent of live cells was determined with 3-(4,5- dimethyl-2-thiazoyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay in human scleral fibroblasts (hFibro), pig trabecular meshwork cells (pTMC), human trabecular meshwork cells (hTMC), and a human retinal pigment epithelial cell line (ARPE-19). Data were normalized to cells not treated with either verteporfin or laser light (0 µg/ml + 0 µJ/cm 2 ; 100% Live), and plotted as the mean (n=4) with error bars representing the standard deviation. *=p<0.05 versus both 0 µg/ml + 0 µJ/cm 2 and pretreat + 50 µJ/cm 2 .

Article Snippet: Verteporfin (Visudyne, QLT Ophthalmics Inc., Menlo Park, CA) came as a lyophilized powder of 15 mg active ingredient in approximately 765 mg of inactive ingredients.

Techniques: Cell Culture, MTT Assay, Standard Deviation