Journal: bioRxiv

Article Title: Arsenite methyltransferase 3 regulates hepatic energy metabolism which dictates the hepatic response to arsenic exposure

doi: 10.1101/2023.04.05.535637

Figure Lengend Snippet: A . Representative images of WT and as3mt -/- larvae and adults. B-C . Standard length of larvae (μM; B) and adults (cm; C). D . Average IC-ICP-MS counts of AsB, AsIII, DMA, and MMA from WT (grey) or as3mt -/- (pink) zebrafish extracts after 0 (dotted lines) or 1 mM iAs (6-120 hpf) challenge (solid lines) (n = 40, 2 clutches). E . Percent survival of WT (grey) and as3mt -/- (pink) across 0, 0.5, 1.0, 1.5 and 2.0 mM iAs exposure from 6-120 hpf. Intersect with green line denotes the LC 50 . n = ∼60, 3 clutches. F . Representative images of WT and as3mt -/- larvae during 0 mM, 1 mM acute (96-120 hpf), or 1 mM chronic (6-120 hpf) exposure to iAs. G . Images of 120 hpf zebrafish pigmentation at 0 mM or 1 mM chronic (6-120 hpf) iAs challenge (purple). Orange arrows mark pigmentation clusters. H . Quantification of pigmentation per larvae (n = 5-10, 1 clutch). I . Percent phenotype (normal (white), abnormal (grey) or dead (black) after 0-2 mM iAs exposure from 6-120 hpf (n = ∼60, 3 clutches).

Article Snippet: IC-ICP-MS standards were: As(III) (Arsenic (III) Standard, Fisher Scientific; 1327-53-3), As(V) (Arsenic V Speciation Standard, Fisher Scientfic; 7732-18-5, AsB (Arsenobetaine Standard Solution, LGC Standards; NIST-3033), DMA (Dimethylarsinic Acid Standard Solution, LGC Standards; NIST-3031) and MMA (Monomethylarsonic Acid Standard Solution, LGC Standards; NIST-3030).

Techniques:

Journal: Electrophoresis

Article Title: Electromigration of Charged Analytes Through Immiscible Fluids in Multiphasic Electrophoresis

doi: 10.1002/elps.202400192

Figure Lengend Snippet: Relationship between protein electrotransfer and oil‐layer viscosity demonstrates a decrease in oil viscosity reduces injection variance. (A) Numerical simulation of protein electrotransfer across oils of varying viscosities, showing that a decrease in oil viscosity facilitates improved protein migration through the thin oil layer. (B) x‐ and y ‐axis variance versus oil‐layer viscosity, with the variance along the y ‐axis being negligible in comparison to the x ‐axis. (C) Péclet ( Pe ) regime analysis for the droplet‐to‐oil and oil‐to‐gel interfaces across different oil viscosities. A decrease in oil viscosity leads to an increase in both Pe and ( L ′/ w ) at the droplet‐to‐oil interface and a decrease at the oil‐to‐gel interface, thus minimizing the variance in protein migration between the droplet‐to‐oil and oil‐to‐gel phases.

Article Snippet: Mineral oil samples with three different viscosities (0.0039, 0.022, and 0.065 Pa s) (ASI Standards) were used as continuous phase.

Techniques: Electrotransfer, Viscosity, Injection, Migration, Comparison

Journal: Electrophoresis

Article Title: Electromigration of Charged Analytes Through Immiscible Fluids in Multiphasic Electrophoresis

doi: 10.1002/elps.202400192

Figure Lengend Snippet: Empirical results of electrophoretic migration of bovine serum albumin (BSA) from aqueous droplets into polyacrylamide gel through a thin oil layer of varying viscosity. (A) Micrographs of protein migration for three different oil viscosities, a decrease in protein migration distance is observed with an increase in mineral oil viscosity. The increase in mineral oil viscosity leads to an increased electrophoretic mobility mismatch among the aqueous, oil, and gel phases, which, in turn, reduces the migration distance (scale bar: 250 µm). (B) Migration distance versus oil‐layer viscosity, a statistically significant difference in migration distance is observed between the 0.0039 Pa s oil and more viscous oil samples. (C) Péclet regime analysis for the droplet‐to‐oil and oil‐to‐gel interfaces across different experimental conditions. A decrease in oil viscosity decreases electrophoretic mobility mismatch among aqueous, oil, and gel phases thus increasing migration distance.

Article Snippet: Mineral oil samples with three different viscosities (0.0039, 0.022, and 0.065 Pa s) (ASI Standards) were used as continuous phase.

Techniques: Migration, Viscosity