Journal: ACS Applied Materials & Interfaces

Article Title: Photo-Cross-linked Gelatin Methacryloyl Hydrogels Enable the Growth of Primary Human Endometrial Stromal Cells and Epithelial Gland Organoids

doi: 10.1021/acsami.4c08763

Figure Lengend Snippet: Viability of stromal cells under various conditions was determined by using the XTT assay. Exposure to 365 nm UV light was ranged over 0–30 min, and exposure to the LAP photoinitiator was tested. Each colored point represents a biological replicate ( n = 3).

Article Snippet: The XTT cell viability kit was obtained from Cell Signaling Technology.

Techniques: XTT Assay

Journal: ACS Applied Materials & Interfaces

Article Title: Photo-Cross-linked Gelatin Methacryloyl Hydrogels Enable the Growth of Primary Human Endometrial Stromal Cells and Epithelial Gland Organoids

doi: 10.1021/acsami.4c08763

Figure Lengend Snippet: EnSC viability and differentiation are supported in DS100 GelMA hydrogels. (A) Schematic overview of experimental design. (B) XTT viability assay on EnSCs cultured for 5 days in 5% and 10% DS100 GelMA hydrogels compared to a collagen control. (C) ELISA quantification of prolactin secretion in DS100 GelMA compared to collagen. Each colored point represents a biological replicate ( n = 3/4).

Article Snippet: The XTT cell viability kit was obtained from Cell Signaling Technology.

Techniques: Viability Assay, Cell Culture, Control, Enzyme-linked Immunosorbent Assay

Journal: Cancers

Article Title: Inhibition of Tumor Microenvironment Cytokine Signaling Sensitizes Ovarian Cancer Cells to Antiestrogen Therapy

doi: 10.3390/cancers14194675

Figure Lengend Snippet: Letrozole and ruxolitinib demonstrate synergy in ovarian cancer cell lines. ERα-high ovarian cancer cell lines OVCAR3 and OVCAR8 were treated with a range of doses of ruxolitinib (Rux) and letrozole (Let) at ratio of 1:2 and cell viability quantified by MTT assay. The combination index (CI) was determined using Compusyn applying the Chou-Talalay method. ( A ) The CI relative to the fraction affected (Fa) is shown for OVCAR3 and OVCAR8. CI at 75% inhibition for each cell line is shown, with values < 1 indicating synergy. ( B ) Colony formation assay for OVCAR8 and graphical representation demonstrating synergy in the colony formation assay.

Article Snippet: Cell viability was determined with the MTT Cell Viability Assay Kit (Biotium, Fremont, CA, USA, Cat. No.30006).

Techniques: MTT Assay, Inhibition, Colony Assay