Journal: International Journal of Molecular Sciences

Article Title: Contribution of Particle-Induced Lysosomal Membrane Hyperpolarization to Lysosomal Membrane Permeabilization

doi: 10.3390/ijms22052277

Figure Lengend Snippet: Comparison of the distribution of DiBAC4(3) in control vs particle-treated AM. Confocal images (A1-E1) and representative examples of fluorescent intensity (A2-E2) of DiBAC4(3) in the lysosomes of live AM. AM were incubated with individual particles for 1 h at 37 °C. ( A1 , A2 ) control, ( B1 , B2 ) treated with 100 µg/mL ZnO, ( C1 , C2 ) treated with 100 µg/mL SiO 2 , ( D1 , D2 ) treated with 100 µg/mL TiO 2 and ( E1 , E2 ) treated with 100 µg/mL CeO 2 . ( F ) Distribution of DiBAC4(3) in the lysosome of control vs treated cells analyzed by one-way ANOVA. ( G ) Distribution of DiBAC4(3) in the cytosol of control vs treated cells analyzed by one-way ANOVA. The fluorescence intensity of at least 100 cells per group was measured and analyzed using ZEN Black imaging software (ZEISS) and ImageJ. Data are presented as means ± SEM of triplicate measurements. ** Indicates significant effect ( p ≤ 0.01) by Holm–Sidak’s multiple comparison test.

Article Snippet: To calculate the ratio correctly, the fluorescent intensity of the dye in at least 100 cells per each group was measured and analyzed using ZEN imaging software (Zen 2.3 SP1 FP3 (black) Version 14.0.21.20, from Zeiss) and ImageJ with individual cell approach.

Techniques: Comparison, Control, Incubation, Fluorescence, Imaging, Software

Journal: International Journal of Molecular Sciences

Article Title: Contribution of Particle-Induced Lysosomal Membrane Hyperpolarization to Lysosomal Membrane Permeabilization

doi: 10.3390/ijms22052277

Figure Lengend Snippet: Hyperpolarization of AM lysosomal membranes after exposure to NP or SiO 2 . AM were incubated with particles for 1 h at 37 °C and lysosomal membrane potential was calculated as discussed in the Methods. A Zeiss LSM 880 confocal microscope and ZEN imaging software (ZEISS) as well as ImageJ were used for our studies. Data are presented as means ± SEM of triplicate measurements. *, ***, and **** indicate significant effects ( p ≤ 0.05, p ≤ 0.001, and p ≤ 0.0001, respectively). Data analyzed by two-way ANOVA and Holm–Sidak’s post hoc test.

Article Snippet: To calculate the ratio correctly, the fluorescent intensity of the dye in at least 100 cells per each group was measured and analyzed using ZEN imaging software (Zen 2.3 SP1 FP3 (black) Version 14.0.21.20, from Zeiss) and ImageJ with individual cell approach.

Techniques: Incubation, Membrane, Microscopy, Imaging, Software