Article Title: A Highly Immunogenic Measles Virus-based Th1-biased COVID-19 Vaccine
Figure Lengend Snippet: Expression of SARS-CoV-2 S protein in Vero and 293T cells. Photographic depiction of fusion activity in Vero or 293T cells 48 h after transfection with 1 µg of SARS-CoV-2 S expression plasmid of control DNA. One representative out of three independent experiments is shown. Scale bar represents 100 mm.
Article Snippet: Cells Vero (African green monkey kidney) (ATCC# CCL-81), Vero clone E6 (ATCC# CRL-1586), 293T (ATCC CRL-3216) and EL-4 (ATCC TIB-39) cell lines were purchased from ATCC (Manassas, VA, USA) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, Biowest, Nuaillé, France) supplemented with 10% fetal bovine serum (FBS; Biochrom, Berlin, Germany) and 2 mM L-glutamine (L-Gln; Biochrom).
Techniques: Expressing, Activity Assay, Transfection, Plasmid Preparation