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Fig. 3. PCV2 vaccination and challenge induces antigen-specific IFN-c secreting cells (PBMC). Pigs were randomly assigned to one of four treatment groups designated T (for treatment) 01–04, n = 24/group. T01, control, received the placebo control, saline; T02 received 2 doses of the cPCV2a/cPCV2b/MH vaccine; T03 received 1 dose of the cPCV2a/ cPCV2b/MH vaccine; and T04 received 2 doses of the rPCV2a/MH vaccine as described in the Study Design table. Pigs were vaccinated (V1 and V2) and challenged (C) on the indicated study days, per treatment. Recall antigen was one of two <t>replicase</t> peptide pools (16-mers, overlapping by 12 amino acids) (A, B) or a set of peptides containing unique T cell epitopes found in PCV2d, but not PCV2a (C). Results expressed as spot forming cells (SFC) per 106 PBMC. Significant differences between groups at each time point are indicated by letter. Groups with different letter designations at each time point are statistically different from each other. Data points without letters, bounded by boxes or with overlapping symbols are not significantly different from each other.
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Fig. 3. PCV2 vaccination and challenge induces antigen-specific IFN-c secreting cells (PBMC). Pigs were randomly assigned to one of four treatment groups designated T (for treatment) 01–04, n = 24/group. T01, control, received the placebo control, saline; T02 received 2 doses of the cPCV2a/cPCV2b/MH vaccine; T03 received 1 dose of the cPCV2a/ cPCV2b/MH vaccine; and T04 received 2 doses of the rPCV2a/MH vaccine as described in the Study Design table. Pigs were vaccinated (V1 and V2) and challenged (C) on the indicated study days, per treatment. Recall antigen was one of two <t>replicase</t> peptide pools (16-mers, overlapping by 12 amino acids) (A, B) or a set of peptides containing unique T cell epitopes found in PCV2d, but not PCV2a (C). Results expressed as spot forming cells (SFC) per 106 PBMC. Significant differences between groups at each time point are indicated by letter. Groups with different letter designations at each time point are statistically different from each other. Data points without letters, bounded by boxes or with overlapping symbols are not significantly different from each other.
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Fig. 3. PCV2 vaccination and challenge induces antigen-specific IFN-c secreting cells (PBMC). Pigs were randomly assigned to one of four treatment groups designated T (for treatment) 01–04, n = 24/group. T01, control, received the placebo control, saline; T02 received 2 doses of the cPCV2a/cPCV2b/MH vaccine; T03 received 1 dose of the cPCV2a/ cPCV2b/MH vaccine; and T04 received 2 doses of the rPCV2a/MH vaccine as described in the Study Design table. Pigs were vaccinated (V1 and V2) and challenged (C) on the indicated study days, per treatment. Recall antigen was one of two <t>replicase</t> peptide pools (16-mers, overlapping by 12 amino acids) (A, B) or a set of peptides containing unique T cell epitopes found in PCV2d, but not PCV2a (C). Results expressed as spot forming cells (SFC) per 106 PBMC. Significant differences between groups at each time point are indicated by letter. Groups with different letter designations at each time point are statistically different from each other. Data points without letters, bounded by boxes or with overlapping symbols are not significantly different from each other.
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Fig. 3. PCV2 vaccination and challenge induces antigen-specific IFN-c secreting cells (PBMC). Pigs were randomly assigned to one of four treatment groups designated T (for treatment) 01–04, n = 24/group. T01, control, received the placebo control, saline; T02 received 2 doses of the cPCV2a/cPCV2b/MH vaccine; T03 received 1 dose of the cPCV2a/ cPCV2b/MH vaccine; and T04 received 2 doses of the rPCV2a/MH vaccine as described in the Study Design table. Pigs were vaccinated (V1 and V2) and challenged (C) on the indicated study days, per treatment. Recall antigen was one of two <t>replicase</t> peptide pools (16-mers, overlapping by 12 amino acids) (A, B) or a set of peptides containing unique T cell epitopes found in PCV2d, but not PCV2a (C). Results expressed as spot forming cells (SFC) per 106 PBMC. Significant differences between groups at each time point are indicated by letter. Groups with different letter designations at each time point are statistically different from each other. Data points without letters, bounded by boxes or with overlapping symbols are not significantly different from each other.
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Fig. 3. PCV2 vaccination and challenge induces antigen-specific IFN-c secreting cells (PBMC). Pigs were randomly assigned to one of four treatment groups designated T (for treatment) 01–04, n = 24/group. T01, control, received the placebo control, saline; T02 received 2 doses of the cPCV2a/cPCV2b/MH vaccine; T03 received 1 dose of the cPCV2a/ cPCV2b/MH vaccine; and T04 received 2 doses of the rPCV2a/MH vaccine as described in the Study Design table. Pigs were vaccinated (V1 and V2) and challenged (C) on the indicated study days, per treatment. Recall antigen was one of two replicase peptide pools (16-mers, overlapping by 12 amino acids) (A, B) or a set of peptides containing unique T cell epitopes found in PCV2d, but not PCV2a (C). Results expressed as spot forming cells (SFC) per 106 PBMC. Significant differences between groups at each time point are indicated by letter. Groups with different letter designations at each time point are statistically different from each other. Data points without letters, bounded by boxes or with overlapping symbols are not significantly different from each other.

Journal: Vaccine

Article Title: Cellular and humoral immunity following vaccination with two different PCV2 vaccines (containing PCV2a or PCV2a/PCV2b) and challenge with virulent PCV2d.

doi: 10.1016/j.vaccine.2021.08.013

Figure Lengend Snippet: Fig. 3. PCV2 vaccination and challenge induces antigen-specific IFN-c secreting cells (PBMC). Pigs were randomly assigned to one of four treatment groups designated T (for treatment) 01–04, n = 24/group. T01, control, received the placebo control, saline; T02 received 2 doses of the cPCV2a/cPCV2b/MH vaccine; T03 received 1 dose of the cPCV2a/ cPCV2b/MH vaccine; and T04 received 2 doses of the rPCV2a/MH vaccine as described in the Study Design table. Pigs were vaccinated (V1 and V2) and challenged (C) on the indicated study days, per treatment. Recall antigen was one of two replicase peptide pools (16-mers, overlapping by 12 amino acids) (A, B) or a set of peptides containing unique T cell epitopes found in PCV2d, but not PCV2a (C). Results expressed as spot forming cells (SFC) per 106 PBMC. Significant differences between groups at each time point are indicated by letter. Groups with different letter designations at each time point are statistically different from each other. Data points without letters, bounded by boxes or with overlapping symbols are not significantly different from each other.

Article Snippet: Table 2 shows sequence identity and divergence of PCV1 or PCV2 by type or PCV2-genotype specific capsid and replicase genes (Clustal W, MegAlign Ver 15.1.0, DNASTAR, Madison, WI, USA).

Techniques: Control, Saline