Journal: bioRxiv

Article Title: Cardiac defects in spinal muscular atrophy and the role of SMN in cardiomyocyte homeostasis

doi: 10.64898/2026.03.20.713246

Figure Lengend Snippet: KO schematic in cardiomyocytes B-D: Oxygen consumption rate (OCR) is significantly increased in siSMN-treated cardiomyocytes compared with siScramble controls and extracellular acidification rate (ECAR) is significantly decreased in siSMN-treated cardiomyocytes. n = 16 technical replicates. * p <0.05. **p < 0.01. Mitochondrial and glycolytic ATP production rates show no significant difference between siSMN and siScramble conditions (ns). E: Volcano plot of differential gene expression following SMN knockdown. F: Heatmap and hierarchical clustering of differentially expressed genes demonstrate distinct transcriptional profiles between siSMN and siScramble cardiomyocytes. G: Pathway enrichment analysis of differentially expressed genes identifies significant perturbation of multiple signaling pathways, including enrichment of PTEN signaling.

Article Snippet: Human cardiomyocytes (Axol Bioscience Limited, Cambridgeshire, England; Cat. No. ax2520; Lot No. 2520310317) were cultured and differentiated in coated plates using supplemented media from the Human iPSC-Derived Ventricular Cardiomyocyte Kit (Axol Bioscience Limited, Cambridgeshire, England).

Techniques: Gene Expression, Knockdown, Protein-Protein interactions

Journal: bioRxiv

Article Title: Cardiac defects in spinal muscular atrophy and the role of SMN in cardiomyocyte homeostasis

doi: 10.64898/2026.03.20.713246

Figure Lengend Snippet: Ingenuity pathway analysis of differentially expressed genes after SMN2 knockdown in human cardiomyocytes, showing the top 20 significantly enriched canonical pathways ranked by –log(p value). Bar color denotes predicted directionality based on z-score: black indicates negative z-score (predicted pathway inhibition), red indicates positive z-score (predicted pathway activation), and gray indicates no consistent activation pattern.

Article Snippet: Human cardiomyocytes (Axol Bioscience Limited, Cambridgeshire, England; Cat. No. ax2520; Lot No. 2520310317) were cultured and differentiated in coated plates using supplemented media from the Human iPSC-Derived Ventricular Cardiomyocyte Kit (Axol Bioscience Limited, Cambridgeshire, England).

Techniques: Knockdown, Inhibition, Activation Assay

Journal: Aging (Albany NY)

Article Title: Systems biology approach to exploring the effect of cyclic stretching on cardiac cell physiology

doi: 10.18632/aging.103465

Figure Lengend Snippet: Differentially expressed genes in human cardiomyocytes. Numbers of differentially expressed genes after cyclic stretching: ( A ) Genes showing an at least 2-fold difference in expression; ( B ) genes showing an at least 1.5-fold difference in expression. Venn diagrams show the overlapping genes with at least 2-fold and 1.5-fold differences in expression.

Article Snippet: Human ventricular cardiomyocyte cells (AC16) were maintained in DMEM/F12 (GeneDireX Incorporation, Taiwan) supplemented with 10% fetal bovine serum (Fisher Scientific, Pittsburgh, PA, USA), 100 IU/mL penicillin (Sigma-Aldrich) and 100 μg/mL streptomycin (Sigma-Aldrich).

Techniques: Expressing

Journal: Aging (Albany NY)

Article Title: Systems biology approach to exploring the effect of cyclic stretching on cardiac cell physiology

doi: 10.18632/aging.103465

Figure Lengend Snippet: Functional analysis of differentially expressed genes in human cardiomyocytes after 1, 4, 12, 24, and 48 hours of cyclic stretching.

Article Snippet: Human ventricular cardiomyocyte cells (AC16) were maintained in DMEM/F12 (GeneDireX Incorporation, Taiwan) supplemented with 10% fetal bovine serum (Fisher Scientific, Pittsburgh, PA, USA), 100 IU/mL penicillin (Sigma-Aldrich) and 100 μg/mL streptomycin (Sigma-Aldrich).

Techniques: Functional Assay

Journal: Aging (Albany NY)

Article Title: Systems biology approach to exploring the effect of cyclic stretching on cardiac cell physiology

doi: 10.18632/aging.103465

Figure Lengend Snippet: Differential expression profile of 29 genes after 1, 4, 12, 24, and 48 hours of cyclical stretching in human cardiomyocytes. The heat map diagram shows expression changes for 29 genes that were altered in cardiac myocytes in response to at least three time points of mechanical stretching.

Article Snippet: Human ventricular cardiomyocyte cells (AC16) were maintained in DMEM/F12 (GeneDireX Incorporation, Taiwan) supplemented with 10% fetal bovine serum (Fisher Scientific, Pittsburgh, PA, USA), 100 IU/mL penicillin (Sigma-Aldrich) and 100 μg/mL streptomycin (Sigma-Aldrich).

Techniques: Quantitative Proteomics, Expressing

Journal: British Journal of Pharmacology

Article Title: Selective late sodium current inhibitor GS‐458967 suppresses Torsades de Pointes by mostly affecting perpetuation but not initiation of the arrhythmia

doi: 10.1111/bph.14217

Figure Lengend Snippet: Late sodium current is lower in ventricular cardiomyocytes of CAVB dog (A) but its inhibition by GS967 (B and C) shortens APD and STV of repolarization (D and E). (A) The TTX‐sensitive current density was lower in ventricular cardiomyocytes isolated from CAVB dogs ( n = 4) than that of SR dogs ( n = 7). (D) Representative example of action potentials recorded in ventricular cardiomyocytes isolated from CAVB dog at baseline and after exposure to 300 nM GS967 (15 action potentials overlaid for both time points). (E) Relative changes in APD and STV of repolarization induced by GS967 in CAVB cardiomyocytes are respectively represented as white and red bars. The number of cardiomyocytes (isolated from 11 CAVB hearts) recorded ( n ) for each concentration is indicated above corresponding bars. Data are represented as mean ± SD. Unpaired t ‐test: * P < 0.05 versus LV SR.

Article Snippet: Cellular experiments were performed on ventricular cardiomyocytes isolated from 15 CAVB dogs (including six animals from the in vivo protocol) and from 11 additional age‐matched Beagle dogs in SR (provided by WIL Research, 's‐Hertogenbosch, The Netherlands) using the whole‐cell patch‐clamp technique.

Techniques: Inhibition, Isolation, Concentration Assay

Journal: British Journal of Pharmacology

Article Title: Selective late sodium current inhibitor GS‐458967 suppresses Torsades de Pointes by mostly affecting perpetuation but not initiation of the arrhythmia

doi: 10.1111/bph.14217

Figure Lengend Snippet: GS967 concentration‐dependently reduces dofetilide‐induced EADs in ventricular cardiomyocytes. In cells exhibiting reproducible dofetilide (1 μM)‐induced EADs, GS967 was superfused at 100, 300 and 1000 nM. (A) Representative example of a successful suppression of dofetilide‐induced EADs by 1000 nM GS967 in a ventricular cardiomyocyte isolated from SR dog. (B) Incidence of EADs after GS967 in the continued presence of dofetilide in ventricular cardiomyocytes isolated from 11 SR and 15 CAVB hearts. Data are presented as number of cells with remaining EADs per number of cells with dofetilide‐induced EADs.

Article Snippet: Cellular experiments were performed on ventricular cardiomyocytes isolated from 15 CAVB dogs (including six animals from the in vivo protocol) and from 11 additional age‐matched Beagle dogs in SR (provided by WIL Research, 's‐Hertogenbosch, The Netherlands) using the whole‐cell patch‐clamp technique.

Techniques: Concentration Assay, Isolation

Journal: iScience

Article Title: Hypoxia inhibits the cardiac I K1 current through SUMO targeting Kir2.1 activation by PIP 2

doi: 10.1016/j.isci.2022.104969

Figure Lengend Snippet:

Article Snippet: Rat ventricular cardiomyocytes from neonatal Wistar rats were purchased from Lonza Biosciences.

Techniques: Recombinant, shRNA, In Situ, Software, Microscopy